Construct: ORF TRCN0000474398
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007385.1_s317c1
- Derived from:
- ccsbBroadEn_10320
- DNA Barcode:
- TGCAGATCTCCTTCAACGTGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH1L2 (160428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474398
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 160428 | ALDH1L2 | aldehyde dehydrogenase 1 fa... | XM_011537986.2 | 62.8% | 62.5% | 1_348del;1804_1834del;1844_2331del |
| 2 | human | 160428 | ALDH1L2 | aldehyde dehydrogenase 1 fa... | XM_017018889.1 | 62.8% | 62.5% | 1_348del;1804_1834del;1844_2331del |
| 3 | human | 160428 | ALDH1L2 | aldehyde dehydrogenase 1 fa... | NM_001034173.4 | 52.8% | 52.6% | 1_786del;2242_2272del;2282_2769del |
| 4 | human | 160428 | ALDH1L2 | aldehyde dehydrogenase 1 fa... | XM_011537988.3 | 47.9% | 47.8% | 1_786del;1865delA;1866_1867ins385 |
| 5 | human | 160428 | ALDH1L2 | aldehyde dehydrogenase 1 fa... | XM_017018890.2 | 33.8% | 33.2% | (many diffs) |
| 6 | human | 160428 | ALDH1L2 | aldehyde dehydrogenase 1 fa... | XM_011537989.3 | 33.7% | 33.2% | (many diffs) |
| 7 | human | 160428 | ALDH1L2 | aldehyde dehydrogenase 1 fa... | NR_027752.2 | 19.8% | 1_804del;2269_7380del | |
| 8 | mouse | 216188 | Aldh1l2 | aldehyde dehydrogenase 1 fa... | XM_011243403.2 | 53.1% | 56.1% | (many diffs) |
| 9 | mouse | 216188 | Aldh1l2 | aldehyde dehydrogenase 1 fa... | NM_153543.2 | 45.1% | 47.7% | (many diffs) |
| 10 | mouse | 216188 | Aldh1l2 | aldehyde dehydrogenase 1 fa... | XR_001779502.1 | 44.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1533
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcactttc tatggctcga cattactgaa tagctctgtg cctcctggag 121 aaccactgga aattaaaggt gccaagaagc ctggtctcgt taccaaaaat ggacttgttc 181 tttttggtaa cgatggaaaa gcactgacgg tgagaaatct gcagtttgaa gatggaaaaa 241 tgatccctgc ctctcagtac ttttcaacgg gtgagacgtc agtggtagaa ctgacagctg 301 aagaggtgaa agtggcagag accatcaagg tcatctgggc tggaatttta agcaatgtcc 361 ccattattga agactcaaca gacttcttta aatctggagc aagctcaatg gatgttgcca 421 ggctggttga agagatcaga cagaaatgtg gtgggcttca gttgcagaat gaagatgtct 481 atatggccac caagtttgaa ggctttatcc aaaaggtcgt gaggaaactg agaggagaag 541 atcaagaggt ggagctggtt gtagattata tttcaaagga ggtcaatgaa atcatggtaa 601 aaatgccata ccagtgtttc ataaatggac agttcacaga tgcagacgat ggaaagactt 661 acgacactat caacccaaca gatggatcta caatatgcaa agtatcctac gcttctttgg 721 cggatgttga taaagcagta gcagcagcaa aagatgcttt tgaaaacggt gaatggggaa 781 gaatgaatgc aagagaaaga ggaagattga tgtatagact tgcagaccta ctggaagaga 841 accaagaaga gctggcaact attgaagccc ttgattcagg ggctgtctat accttggccc 901 tgaagacaca cattggaatg tctgtgcaaa cattcagata ttttgctggc tggtgcgaca 961 aaattcaggg ttctactatt ccaatcaacc aggcccgtcc aaatcgcaat ctgaccttca 1021 ccAAGAAAGA GCCACTCGGT GTCTGTGCCA TTATTATTCC CTGGAACTAC CCGCTGATGA 1081 TGCTGGCATG GAAGAGTGCT GCGTGTTTGG CAGCAGGCAA TACCTTAGTG CTCAAGCCAG 1141 CACAGGTCAC GCCCTTGACT GCTTTGAAGT TTGCAGAACT GTCTGTGAAA GCAGGCTTTC 1201 CAAAGGGGGT CATCAACATC ATTCCAGGCT CAGGTGGCAT AGCAGGACAA CGTCTGTCTG 1261 AACATCCTGA CATCCGCAAA CTTGGTTTCA CTGGATCCAC TCCTATTGGC AAACAGATCA 1321 TGAAGAGCTG TGCTGTTAGC AACTTGAAGA AAGTTTCCCT TGAGCTTGGT GGCAAGTCTC 1381 CACTTATAAT ATTTAATGAC TGTGAACTTG ACAAGGCTGT GCGAATGGGC ATGGGAGCAG 1441 TATTTTTCAA CAAAGGAGAG AACTGTATTG CTGCTGGGCG GTTGTTCGTG GAAGAATCCA 1501 TCCACGACGA ATTTGTGACA AGAGTGATCC ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1561 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1621 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 1681 CAGATCTCCT TCAACGTGGC GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1741 ttgtgaaaga tt