Construct: ORF TRCN0000474402
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015170.1_s317c1
- Derived from:
- ccsbBroadEn_10360
- DNA Barcode:
- ATCCGACGCAGTTTGAGACACATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPY19L2P2 (349152)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474402
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448957.1 | 27.5% | 20% | (many diffs) |
| 2 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448955.1 | 26% | 18.9% | (many diffs) |
| 3 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448954.1 | 25.2% | 18.4% | (many diffs) |
| 4 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448953.1 | 21.6% | 15.8% | (many diffs) |
| 5 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448952.1 | 20.9% | 15.3% | (many diffs) |
| 6 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448951.1 | 20.8% | 15.2% | (many diffs) |
| 7 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448950.1 | 20.2% | 14.8% | (many diffs) |
| 8 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448949.1 | 19.8% | 14.5% | (many diffs) |
| 9 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448948.1 | 18.8% | 13.7% | (many diffs) |
| 10 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_017019192.2 | 15.8% | 11.6% | (many diffs) |
| 11 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448945.1 | 15.6% | 11.5% | (many diffs) |
| 12 | human | 442523 | DPY19L2P4 | DPY19L2 pseudogene 4 | NR_003551.1 | 15.3% | (many diffs) | |
| 13 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448944.1 | 15.2% | 11.1% | (many diffs) |
| 14 | human | 283417 | DPY19L2 | dpy-19 like 2 | NM_173812.5 | 14.8% | 10.8% | (many diffs) |
| 15 | human | 554236 | DPY19L2P1 | DPY19L2 pseudogene 1 | NR_002833.2 | 12.6% | (many diffs) | |
| 16 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957316.1 | 9% | (many diffs) | |
| 17 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957315.1 | 8.7% | (many diffs) | |
| 18 | human | 349152 | DPY19L2P2 | DPY19L2 pseudogene 2 | NR_027768.1 | 8.3% | 1_314del;681_4361del | |
| 19 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957314.1 | 8.1% | (many diffs) | |
| 20 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957318.1 | 3.1% | (many diffs) | |
| 21 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957319.1 | 3% | (many diffs) | |
| 22 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_001748666.2 | 2.9% | (many diffs) | |
| 23 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957317.1 | 2.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 89
- ORF end:
- 455
- ORF length:
- 366
- Sequence:
-
1 CAGACAGTGG TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG 61 ATCAACAAGT TTGTACAAAA AAGTTGGCAT GAAGAAACAA GGAGTAAGCC CAAAGCCGCT 121 GCAATCTTCC CGCCCCAGCC CGTCTAAGCG GCCCTGCGGG GCCTCCCCCG CCCGGGAGCG 181 GGAGGTGGAA AAGTCGGCCC TAGGCGGCGG GAAACTGCCG GGGGGCGCCA GGAGGTCCTC 241 CCCGGGGAGG ATCCCAAATC TGAAAAAGCG AAAAGGCTTG GAGCTAAAGG TGGTGGCCAA 301 GGCCCTTCTC GGCCCCTTCC AGTTCGTCTG TAATTCCCTG GCGCAGCTCC GGGAAGAGGT 361 GCACGAACTG CAGGCGCGGT GGTTCCCCAG CAGAACCACT CTGCATCGCC GTCTTTGTGG 421 CAATTCTACA TTGGTTACAT TTAGTAACAC TTTTTGCCCA ACTTTCTTGT ACAAAGTGGT 481 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 541 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 601 ATCCGACGCA GTTTGAGACA CATCACGCGT TAAGTCgaca atcaacctct ggattacaaa 661 atttgtgaaa gatt