Construct: ORF TRCN0000474507
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004776.1_s317c1
- Derived from:
- ccsbBroadEn_13602
- DNA Barcode:
- TACTAACTTACGCTAGCGGCTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LHFPL1 (340596)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474507
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340596 | LHFPL1 | LHFPL tetraspan subfamily m... | XM_011530946.2 | 100% | 100% | |
| 2 | human | 340596 | LHFPL1 | LHFPL tetraspan subfamily m... | NM_178175.4 | 85% | 85% | 381_479del |
| 3 | human | 340596 | LHFPL1 | LHFPL tetraspan subfamily m... | XM_011530944.2 | 85% | 85% | 381_479del |
| 4 | human | 340596 | LHFPL1 | LHFPL tetraspan subfamily m... | XM_011530943.2 | 76.9% | 76.9% | 1_69del;450_548del |
| 5 | human | 340596 | LHFPL1 | LHFPL tetraspan subfamily m... | XM_017029485.1 | 76.9% | 76.9% | 1_69del;450_548del |
| 6 | human | 340596 | LHFPL1 | LHFPL tetraspan subfamily m... | XM_024452370.1 | 71.2% | 63.1% | (many diffs) |
| 7 | mouse | 237091 | Lhfpl1 | lipoma HMGIC fusion partner... | XM_006528803.2 | 90.3% | 94.6% | (many diffs) |
| 8 | mouse | 237091 | Lhfpl1 | lipoma HMGIC fusion partner... | NM_178358.3 | 76.8% | 80.4% | (many diffs) |
| 9 | mouse | 237091 | Lhfpl1 | lipoma HMGIC fusion partner... | XM_006528800.2 | 76.8% | 80.4% | (many diffs) |
| 10 | mouse | 237091 | Lhfpl1 | lipoma HMGIC fusion partner... | XM_006528801.3 | 76.8% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 630
- ORF length:
- 561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggagcagc ctgaCCATGG TGGGAACCCT CTGGGCCTTC CTGTCCCTTG 121 TTACTGCTGT GACCAGTTCT ACCAGTTACT TCCTACCTTA CTGGCTCTTT GGATCCCAGA 181 TGGGGAAGCC AGTGTCATTC AGCACATTCC GGAGGTGCAA CTACCCTGTG CGGGGAGAGG 241 GACACAGTCT GATCATGGTG GAAGAATGTG GGCGCTATGC CAGCTTCAAT GCCATCCCAA 301 GCCTGGCCTG GCAGATGTGC ACAGTGGTGA CAGGTGCCGG CTGTGCTCTG CTGCTCCTGG 361 TGGCACTAGC TGCTGTCCTG GGTTGCTGCA TGGAGGAGCT CATCTCCAGA ATGATGGGAC 421 GTTGCATGGG AGCAGCGCAG TTTGTTGGAG GTACCTGTCG GCTTGGCTGG GCCTATTACT 481 GTGCTGGAGG TGGAGCAGCT GCAGCCATGT TGATCTGCAC CTGGCTCTCT TGCTTTGCTG 541 GAAGAAACCC CAAGCCTGTC ATATTGGTGG AGAGCATCAT GAGGAATACC AATTCTTATG 601 CTATGGAGCT TGACCATTGC CTCAAACCTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATACTA 781 ACTTACGCTA GCGGCTCCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt