Transcript: Mouse XM_006528800.2

PREDICTED: Mus musculus lipoma HMGIC fusion partner-like 1 (Lhfpl1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lhfpl1 (237091)
Length:
1753
CDS:
304..966

Additional Resources:

NCBI RefSeq record:
XM_006528800.2
NBCI Gene record:
Lhfpl1 (237091)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006528800.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000248046 TAGACTCGGCTGGGCCTATTA pLKO_005 792 CDS 100% 13.200 18.480 N Lhfpl1 n/a
2 TRCN0000248049 CGGGAATGTCTCCAATCAATT pLKO_005 756 CDS 100% 13.200 10.560 N Lhfpl1 n/a
3 TRCN0000200696 GTGCATTATATCCTTTAGGAT pLKO.1 707 CDS 100% 3.000 2.400 N Lhfpl1 n/a
4 TRCN0000248047 AGCGCAGGCTGTGCATTATAT pLKO_005 697 CDS 100% 15.000 10.500 N Lhfpl1 n/a
5 TRCN0000217806 CCAGTATCTTTCAGCACATTC pLKO.1 424 CDS 100% 10.800 7.560 N Lhfpl1 n/a
6 TRCN0000248045 GTGGCCAGTTCTACCAGTTAC pLKO_005 364 CDS 100% 10.800 7.560 N Lhfpl1 n/a
7 TRCN0000200825 GCACATCACAAGAATTACTAT pLKO.1 1411 3UTR 100% 5.625 3.938 N Lhfpl1 n/a
8 TRCN0000191199 CCAATCAATTTCAGTTAGGAA pLKO.1 767 CDS 100% 3.000 2.100 N Lhfpl1 n/a
9 TRCN0000248048 ATGTACTTCCCACTTACTTTA pLKO_005 1274 3UTR 100% 13.200 7.920 N Lhfpl1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006528800.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05477 pDONR223 100% 90.9% 94.5% None (many diffs) n/a
2 ccsbBroad304_05477 pLX_304 0% 90.9% 94.5% V5 (many diffs) n/a
3 TRCN0000480663 GATCCGAGCACACTCAAGCAAAAA pLX_317 55% 90.9% 94.5% V5 (many diffs) n/a
4 ccsbBroadEn_13602 pDONR223 100% 76.8% 80.4% None (many diffs) n/a
5 ccsbBroad304_13602 pLX_304 0% 76.8% 80.4% V5 (many diffs) n/a
6 TRCN0000474507 TACTAACTTACGCTAGCGGCTCCT pLX_317 95.2% 76.8% 80.4% V5 (many diffs) n/a
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