Construct: ORF TRCN0000474512
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008143.1_s317c1
- Derived from:
- ccsbBroadEn_12545
- DNA Barcode:
- GGGAGGGCCCAAAGGTCCATTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM48 (79097)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474512
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79097 | TRIM48 | tripartite motif containing 48 | NM_024114.5 | 92.7% | 92.8% | 1_48del;468T>G |
| 2 | human | 642612 | TRIM49C | tripartite motif containing... | XM_017018126.1 | 51.7% | 48% | (many diffs) |
| 3 | human | 57093 | TRIM49 | tripartite motif containing 49 | XM_017018027.2 | 51.6% | 48% | (many diffs) |
| 4 | human | 283116 | TRIM49B | tripartite motif containing... | XM_017017561.1 | 51.4% | 47.7% | (many diffs) |
| 5 | human | 729384 | TRIM49D2 | tripartite motif containing... | NM_001105522.1 | 45% | 43.5% | (many diffs) |
| 6 | human | 399939 | TRIM49D1 | tripartite motif containing... | NM_001206627.1 | 45% | 43.5% | (many diffs) |
| 7 | human | 399939 | TRIM49D1 | tripartite motif containing... | XM_011542816.3 | 45% | 43.5% | (many diffs) |
| 8 | human | 642612 | TRIM49C | tripartite motif containing... | NM_001195234.1 | 42.9% | 39.8% | (many diffs) |
| 9 | human | 57093 | TRIM49 | tripartite motif containing 49 | NM_020358.2 | 42.8% | 39.8% | (many diffs) |
| 10 | human | 642612 | TRIM49C | tripartite motif containing... | XM_024448656.1 | 42.7% | 38.1% | (many diffs) |
| 11 | human | 283116 | TRIM49B | tripartite motif containing... | NM_001206626.1 | 42.6% | 39.6% | (many diffs) |
| 12 | human | 57093 | TRIM49 | tripartite motif containing 49 | XM_024448617.1 | 42.6% | 38.1% | (many diffs) |
| 13 | human | 84767 | TRIM51 | tripartite motif-containing 51 | NM_032681.4 | 40.5% | 34.7% | (many diffs) |
| 14 | human | 642569 | TRIM53AP | tripartite motif containing... | NR_028346.1 | 34.1% | (many diffs) | |
| 15 | human | 399940 | TRIM51EP | tripartite motif-containing... | NR_146921.1 | 32.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttctggaatc tcgcaagtct tccagaggga actcacctgc cccatctgca 121 tgaactactt catagacccg gtcaccatag actgtgggca cagcttttgc aggccctgtt 181 tctaccTCAA CTGGCAAGAC ATCCCAATTC TTACTCAGTG CTTTGAATGC ATAAAGACAA 241 TACAGCAGAG AAACCTCAAA ACTAACATTC GATTGAAGAA GATGGCTTCC CTTGCCAGAA 301 AAGCCAGTCT CTGGCTATTC CTGAGCTCTG AGGAGCAAAT GTGTGGCATT CACAGGGAGA 361 CAAAGAAGAT GTTCTGTGAA GTGGACAGGA GCCTGCTCTG TTTGCTGTGC TCCAGCTCTC 421 AGGAGCACCG GTATCACAGA CACTGTCCCG CTGAGTGGGC TGCTGAGGAA CACTGGGAGA 481 AGCTGTTAAA GAAAATGCAG TCTTTATGGG AAAAAGCTTG TGAAAATCAG AGAAACCTGA 541 ATGTGGAAAC CACCAGAATC AGCCACTGGA AGGCTTTTGG AGACATATTA TACAGGAGTG 601 AGTCCGTGCT GCTGCACATG CCCCAGCCTC TGAATCTAGC GCTCAGGGCA GGGCCCATCA 661 CTGGACTGAG GGACAGGCTC AACCAATTCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 721 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 781 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGGAG 841 GGCCCAAAGG TCCATTTTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 901 tgaaagatt