Construct: ORF TRCN0000474534

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003715.1_s317c1
Derived from:
ccsbBroadEn_11297
DNA Barcode:
TAATTCATAGTAGGTCAAAAGAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NOL4 (8715)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474534
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8715 NOL4 nucleolar protein 4 NM_001353234.1 84.4% 84.4% 1_120del;1015_1128del
2 human 8715 NOL4 nucleolar protein 4 NM_001282527.1 82.8% 82.8% 0_1ins123;772_885del
3 human 8715 NOL4 nucleolar protein 4 NM_001353237.1 82.8% 82.8% 0_1ins123;772_885del
4 human 8715 NOL4 nucleolar protein 4 XM_017026055.2 82.8% 82.8% 0_1ins123;772_885del
5 human 8715 NOL4 nucleolar protein 4 XM_011526238.2 80.5% 80.5% 895_1200del
6 human 8715 NOL4 nucleolar protein 4 XM_024451279.1 80.5% 80.5% 895_1200del
7 human 8715 NOL4 nucleolar protein 4 NM_001198548.1 78.7% 78.7% 1_342del
8 human 8715 NOL4 nucleolar protein 4 XM_017026052.2 75.7% 75.6% (many diffs)
9 human 8715 NOL4 nucleolar protein 4 NM_001198547.1 74.8% 74.8% 1_120del;1015_1320del
10 human 8715 NOL4 nucleolar protein 4 NM_001198546.1 73.5% 73.5% 1_342del;1237_1350del
11 human 8715 NOL4 nucleolar protein 4 NM_001353235.1 72.7% 72.7% 0_1ins123;772_1077del
12 human 8715 NOL4 nucleolar protein 4 XM_011526239.3 72.7% 72.7% 0_1ins123;772_1077del
13 human 8715 NOL4 nucleolar protein 4 XM_017026053.2 72.7% 72.7% 0_1ins123;772_1077del
14 human 8715 NOL4 nucleolar protein 4 XM_017026054.2 72.7% 72.7% 0_1ins123;772_1077del
15 human 8715 NOL4 nucleolar protein 4 XM_017026058.2 72.7% 72.7% 0_1ins123;772_1077del
16 human 8715 NOL4 nucleolar protein 4 NM_003787.4 66.1% 66.1% 1_342del;1237_1542del
17 human 8715 NOL4 nucleolar protein 4 XM_006722563.3 66.1% 66.1% 1_342del;1237_1542del
18 human 8715 NOL4 nucleolar protein 4 XM_011526237.2 66.1% 66.1% 1_342del;1237_1542del
19 human 8715 NOL4 nucleolar protein 4 XM_017026060.2 61% 60.3% (many diffs)
20 human 8715 NOL4 nucleolar protein 4 NM_001353232.1 59.5% 58.8% (many diffs)
21 human 8715 NOL4 nucleolar protein 4 XR_935261.3 56.6% (many diffs)
22 human 8715 NOL4 nucleolar protein 4 NM_001353236.1 54.5% 54.5% 0_1ins513;382_495del
23 human 8715 NOL4 nucleolar protein 4 NM_001353233.1 53.6% 53% (many diffs)
24 human 8715 NOL4 nucleolar protein 4 NM_001198549.1 47.9% 47.9% 0_1ins513;382_687del
25 human 8715 NOL4 nucleolar protein 4 XM_017026059.2 47.9% 47.9% 0_1ins513;382_687del
26 human 8715 NOL4 nucleolar protein 4 XR_002958188.1 32.3% (many diffs)
27 mouse 319211 Nol4 nucleolar protein 4 XM_017317922.1 72.8% 76.3% (many diffs)
28 mouse 319211 Nol4 nucleolar protein 4 XM_017317923.1 72.8% 76.3% (many diffs)
29 mouse 319211 Nol4 nucleolar protein 4 XM_006525999.2 71.2% 74.8% (many diffs)
30 mouse 319211 Nol4 nucleolar protein 4 NM_001161483.1 66% 69.3% (many diffs)
31 mouse 319211 Nol4 nucleolar protein 4 NM_001347510.1 65% 68.2% (many diffs)
32 mouse 319211 Nol4 nucleolar protein 4 XM_017317921.1 63.9% 66.9% (many diffs)
33 mouse 319211 Nol4 nucleolar protein 4 NM_001347509.1 58.5% 61.4% (many diffs)
34 mouse 319211 Nol4 nucleolar protein 4 NM_199024.2 51.1% 52.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1332
ORF length:
1266
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca tgtggaaacg gggccaaatg gagaacaaat tcggaaacac gctggacaaa
121agagaactta caaagcaatt tcagagagct atgccttcct accaagagaa gcggtgacac
181gatttctaat gagctgctca gagtgccaga aaagaatgca tttaaaccca gatggaacag
241atcataaaga taatggaaaa cctcccactt tggtgaccag catgattgac tacaacatgc
301caattaccat ggcctacatg aaacacatga agctgcagct gctaaactca cagcaagatg
361aggatgaaag ttcaatagaa agtgatgaat ttgacatgag tgattcaaca cggatgtcag
421ctgtgaactc tgatcttagc tccaatcttg aagaaagaat gcaaagtccc cagaatcttc
481atggccagca agatgatgat tctgctgcag agagctttaa tggcaatgag actctggggc
541acagttcaat tgcttcaggg ggaacacaca gcagggagat gggagactcc aacagtgatg
601gcaaaactgg gctggagcaa gatgaacagc cactgaacct gagtgacagt cccctctctg
661cgcagctaac ttcggaatac agaatagatg atcacaacag taatgggaaa aacaagtata
721agaatcttct aatttctgac ctcaagatgg aacgagaggc gagagaaaat ggaagcaagt
781ctcctgcaca tagttactcc agctatgacT CTGGCAAAAA TGAGAGTGTA GACCGAGGAG
841CTGAGGACCT CTCACTAAAC AGGGGAGATG AGGACGAAGA TGACCACGAG GACCATGACG
901ATTCGGAGAA AGTTAATGAG ACAGACGGCG TTGAAGCCGA GCGGCTGAAA GCTTTTAATG
961ATGAGTCTGC TCCAGCTGAC AAACAGTGTA AACCAGAGGC GACCCAGGCC ACTTACTCAA
1021CATCAGCTGT TCCAGGCTCA CAGGACGTGC TGTACATCAA TGGAAATGGG ACCTATAGTT
1081ACCATAGTTA CAGAGGGCTA GGAGGGGGTC TGCTAAATCT GAATGATGCT TCCAGCAGTG
1141GACCCACTGA TCTCAGCATG AAGAGACAAT TGGCGACTAG CTCAGGATCC TCCAGCAGCT
1201CAAACTCCAG ACCCCAGCTG AGTCCAACTG AAATCAATGC CGTGAGACAG CTTGTTGCAG
1261GATATCGAGA ATCAGCTGCA TTTTTATTGC GATCTGCAGA TGAACTGGAA AATCTCATTT
1321TACAACAGAA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1381ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1441CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAA TTCATAGTAG GTCAAAAGAA
1501CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)