Construct: ORF TRCN0000474576
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007214.1_s317c1
- Derived from:
- ccsbBroadEn_13842
- DNA Barcode:
- CCGCATCAATTTCGCCTCTGTCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DACH1 (1602)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474576
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1602 | DACH1 | dachshund family transcript... | NM_080759.6 | 73.1% | 2.4% | 1_567del;580_581insG |
| 2 | human | 1602 | DACH1 | dachshund family transcript... | XM_011534942.3 | 70.5% | 6% | 0_1ins457 |
| 3 | human | 1602 | DACH1 | dachshund family transcript... | NM_001366712.1 | 68.1% | 2.2% | 1_567del;580_581insG;1126_1281del |
| 4 | human | 1602 | DACH1 | dachshund family transcript... | XM_017020396.1 | 64.1% | 4.6% | 0_1ins457;103_258del |
| 5 | human | 1602 | DACH1 | dachshund family transcript... | NM_080760.5 | 52.2% | 3% | 1_567del;580_581insG;1125_1126ins444 |
| 6 | human | 1602 | DACH1 | dachshund family transcript... | XM_011534940.2 | 46.4% | 2.8% | (many diffs) |
| 7 | human | 1602 | DACH1 | dachshund family transcript... | NM_004392.6 | 44.5% | 3.3% | 1_567del;580_581insG;962_963ins606 |
| 8 | human | 1602 | DACH1 | dachshund family transcript... | XM_011534941.2 | 26.7% | 4.4% | (many diffs) |
| 9 | mouse | 13134 | Dach1 | dachshund 1 (Drosophila) | NM_001038610.2 | 68.4% | 3% | (many diffs) |
| 10 | mouse | 13134 | Dach1 | dachshund 1 (Drosophila) | NM_007826.3 | 63.7% | 2.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 330
- ORF length:
- 264
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggatctgagg gggggccaaa gtggcttcct tcacggtgga gggctgcgag 121 ctgatctgcc tgccccaggc tttcgacctg ttcctgaagc acttggtggg gggcttgcat 181 acggtctaca ccaagctgaa gcggctggag atcacgccgg tggtgtgcaa tgtggaacaa 241 gttcgcatcc tgaggggact gggcgccatc cagccaggag tgaaccgctg caaactcatc 301 tccaggaagg acttcgagac cctctacaat gactgcacca acgcaagttc tagacctgga 361 aggcctccta agaggactca aagtgtcacc tccccagaga actctcacat catgccgcat 421 tctgtccctg gtctcatgtc tcctgggata attccaccaa caggtctgac agcagccgct 481 gcagcagctg ctgctgctac caatgcagct attgctgaag caatgaaggt gaaaaaaatc 541 aaattagaag ccatgagcaa ctatcatgcc agtaataacc aacatggagc agactctgaa 601 aacggggaca tgaattcaag tgtcggactg gaacttcctt ttatgatgat gccccaccct 661 ctaattcctg tcagcctacc tccagcatct gtcaccatgg caatgagcca gatgaaccac 721 ctcagcacca ttgcaaatat ggcagcagca gcacaagttc agagtccccc atccagagtt 781 gagacatcag ttattaagga gcgtgttcct gatagcccct cacctgcccc ctctctggag 841 gaggggagaa ggcctggcag tcacccatca tcacatcgca gcagcagcgt gtccagctcc 901 cctgctcgga ctgagagctc ttctgacaga atcccggtcc atcagaatgg gttgtccatg 961 aaccagatgc tgatgggctt atcaccaaat gtacttcctg ggcccaaaga gggagatttg 1021 gccggtcatg acatgggaca tgagtcaaaa aggatgcata ttgaaaaaga tgagaccccg 1081 ctttctacac caaccgcaag agacagcctt gacaaactct ctctaactgg gcatggacaa 1141 ccactgcctc caggttttcc atctcctttt ctgtttcctg atggactgtc ttccatcgag 1201 actcttctga ctaacataca ggggctgttg aaagttgcca tagataatgc cagagctcaa 1261 gagaaacagg tccaactgga aaaaactgag ctgaagatgg attttttaag ggaaagagaa 1321 ctaagggaaa cacttgagaa gcagttGGCT ATGGAACAAA AGAATAGAGC CATAGTTCAA 1381 AAGAGGCTAA AGAAGGAGAA GAAGGCAAAG AGAAAATTGC AGGAAGCACT TGAGTTTGAG 1441 ACGAAACGGC GTGAACAAGC AGAACAGACG CTAAAACAGG CAGCTTCAAC AGATAGTCTC 1501 AGGGTCTTAA ATGACTCTCT GACCCCAGAG ATAGAGGCTG ACCGCAGTGG CGGCAGAACA 1561 GATGCTGAAA GGACAATACA AGATGGAAGA CTGTATTTGA AAACTACTGT CATGTACTGC 1621 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 1681 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1741 TATATATCTT GTGGAAAGGA CGACCGCATC AATTTCGCCT CTGTCTCACG CGTTAAGTCg 1801 acaatcaacc tctggattac aaaatttgtg aaagatt