Construct: ORF TRCN0000474619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001869.1_s317c1
- Derived from:
- ccsbBroadEn_11438
- DNA Barcode:
- TGAACAGTTAGGAGAACGGCCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC23A1 (9963)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474619
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9963 | SLC23A1 | solute carrier family 23 me... | XM_011543769.1 | 61.8% | 51.5% | (many diffs) |
| 2 | human | 9963 | SLC23A1 | solute carrier family 23 me... | XM_011543767.1 | 48.5% | 48.5% | 1_108del;378_1091del |
| 3 | human | 9963 | SLC23A1 | solute carrier family 23 me... | XM_011543768.1 | 45.8% | 38.3% | (many diffs) |
| 4 | human | 9963 | SLC23A1 | solute carrier family 23 me... | XM_011543766.1 | 45.8% | 45.8% | 1_108del;378_1187del |
| 5 | human | 9963 | SLC23A1 | solute carrier family 23 me... | NM_005847.5 | 43.3% | 43.3% | 270_1286del |
| 6 | human | 9963 | SLC23A1 | solute carrier family 23 me... | NM_152685.3 | 43% | 43% | 270_1298del |
| 7 | human | 9963 | SLC23A1 | solute carrier family 23 me... | XM_005272149.4 | 40.8% | 40.8% | 1_108del;378_1394del |
| 8 | human | 9963 | SLC23A1 | solute carrier family 23 me... | XM_005272148.3 | 40.5% | 40.5% | 1_108del;378_1406del |
| 9 | human | 9963 | SLC23A1 | solute carrier family 23 me... | XM_011543765.2 | 40.5% | 40.5% | 1_108del;378_1406del |
| 10 | human | 9963 | SLC23A1 | solute carrier family 23 me... | XM_006714741.2 | 35.5% | 35.5% | 1_108del;378_1406del;1570_1571ins96 |
| 11 | mouse | 20522 | Slc23a1 | solute carrier family 23 (n... | NM_011397.4 | 36.9% | 36.6% | (many diffs) |
| 12 | mouse | 20522 | Slc23a1 | solute carrier family 23 (n... | XM_017317851.1 | 36.9% | 36.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggcccaggag gacctcgagg gccggacaca gcatgaaacc accagggacc 121 cctcgacccc gctacccaca gagcctaagt ttgacatgtt gtacaagatc gaggacgtgc 181 caccttggta cctgtgcatc ctgctgggct tccagcacta cctgacatgc ttcagtggta 241 ccatcgccgt gcccttcctg ctggctgagg cgctgtgtgt gggccacgac cagcacatgg 301 ttagtcagct catcGGCACC ATCTTCACGT GCGTGGGCAT GTTCTGCACT CTCTTTGGCA 361 TGATTACAGC TGTGGGGCTG TCCAACCTGC AATTTGTGGA CATGAACTCC TCTCGCAACC 421 TCTTCGTGCT GGGATTTTCC ATGTTCTTCG GGCTCACGCT GCCCAATTAC CTGGAGTCCA 481 ACCCTGGCGC CATCAATACA GGCATTCTTG AAGTGGATCA GATTCTGATT GTGCTGCTGA 541 CCACGGAGAT GTTTGTGGGC GGGTGCCTTG CTTTCATACT TGACAACACA GTGCCAGGGA 601 GCCCAGAGGA GCGTGGTCTG ATACAGTGGA AAGCTGGGGC TCATGCCAAC AGTGACATGT 661 CTTCCAGCCT CAAGAGCTAC GATTTCCCCA TTGGGATGGG CATAGTAAAA AGAATTACCT 721 TTCTGAAATA CATTCCTATC TGCCCAGTCT TCAAAGGATT TTCTTCAAGT TCAAAAGATC 781 AGATTGCAAT TCCAGAAGAC ACTCCAGAAA ATACAGAAAC TGCATCTGTG TGCACCAAGG 841 TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGATG AACAGTTAGG AGAACGGCCT GGACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt