Construct: ORF TRCN0000474620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003393.1_s317c1
- Derived from:
- ccsbBroadEn_12790
- DNA Barcode:
- GCGGACCCCCACCCACACATATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SETD3 (84193)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474620
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84193 | SETD3 | SET domain containing 3, ac... | NM_199123.2 | 72.2% | 72.2% | 1_246del |
| 2 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537235.1 | 72.2% | 72.2% | 1_246del |
| 3 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_005268127.3 | 36.5% | 34.3% | (many diffs) |
| 4 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537234.1 | 36.5% | 34.3% | (many diffs) |
| 5 | human | 84193 | SETD3 | SET domain containing 3, ac... | NM_032233.3 | 35.3% | 34.5% | (many diffs) |
| 6 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537231.2 | 35.3% | 34.5% | (many diffs) |
| 7 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537232.3 | 35.3% | 34.5% | (many diffs) |
| 8 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_017021699.1 | 35.3% | 34.5% | (many diffs) |
| 9 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_017021700.1 | 35.3% | 34.5% | (many diffs) |
| 10 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_017021702.1 | 34.7% | 27.2% | (many diffs) |
| 11 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537233.2 | 33.7% | 28.5% | (many diffs) |
| 12 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_017021701.1 | 33.7% | 28.5% | (many diffs) |
| 13 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516084.3 | 54% | 50.5% | (many diffs) |
| 14 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516080.3 | 34.6% | 36% | (many diffs) |
| 15 | mouse | 52690 | Setd3 | SET domain containing 3 | NM_028262.3 | 32.7% | 34% | (many diffs) |
| 16 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516079.3 | 32.3% | 33.6% | (many diffs) |
| 17 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516078.3 | 29.7% | 28.9% | (many diffs) |
| 18 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516082.3 | 29.3% | 30.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa atgggcctct gaaaatgggg cttctgtcga gggttttgaa atggttaact 121 tcaaagaaga gggctttggt ttgagagcaa caagagatat caaggcagaa gaattgtttt 181 tatgggttcc acgaaaattg ctaatgactg ttgaatctgc taaaaattca gtgttggggc 241 ccttatattc tcaagaccga atccttcaag ccatgggaaa catcgcactg gcctttcatt 301 tgctgtgtga gcgagccagc cctaactcct tctggcagcc ctatattcaa accctcccca 361 gtgaatatga cactcctctc tactttgaag aagatgaagt tcggtatctt cagtccacac 421 aagctataca tgatgtcttc agccagtata aaaacacagc tcGACAGTAC GCCTACTTCT 481 ATAAAGTCAT CCAGACCCAT CCTCATGCCA ACAAACTACC CTTGAAGGAT TCTTTCACTT 541 ACGAGGACTA CAGGTGGGCA GTCTCTTCTG TTATGACGAG GCAAAACCAA ATTCCCACAG 601 AGGATGGTTC CCGCGTGACC CTGGCTCTGA TTCCTTTATG GGATATGTGT AACCACACCA 661 ACGGCCTGAC ACCAGAAGAT TCCTTCGCTC TTGCTGTCGC CTCTGCCTAC CCAACTTTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGAGCGGACC CCCACCCACA CATATACACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt