Construct: ORF TRCN0000474650
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010237.1_s317c1
- Derived from:
- ccsbBroadEn_01243
- DNA Barcode:
- ACAATCGTGAGTCTTCATTCAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR2J (5439)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474650
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5439 | POLR2J | RNA polymerase II subunit J | NM_006234.5 | 100% | 100% | |
| 2 | human | 5439 | POLR2J | RNA polymerase II subunit J | XM_017012353.1 | 99.1% | 99.1% | 46_47insAGA |
| 3 | human | 548644 | POLR2J3 | RNA polymerase II subunit J3 | NM_001097615.2 | 85.5% | 86% | (many diffs) |
| 4 | human | 246721 | POLR2J2 | RNA polymerase II subunit J2 | NM_032959.6 | 85% | 85.2% | (many diffs) |
| 5 | human | 5439 | POLR2J | RNA polymerase II subunit J | NM_001371100.1 | 74.3% | 74.3% | 53_54ins90 |
| 6 | human | 5439 | POLR2J | RNA polymerase II subunit J | XM_005250452.5 | 70.4% | 68.5% | (many diffs) |
| 7 | human | 5439 | POLR2J | RNA polymerase II subunit J | NR_163857.1 | 36.3% | 1_62del;116_144del;443_966del | |
| 8 | human | 84820 | POLR2J4 | RNA polymerase II subunit J... | NR_003655.3 | 5% | (many diffs) | |
| 9 | mouse | 20022 | Polr2j | polymerase (RNA) II (DNA di... | NM_011293.2 | 89.7% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cgccccTCCA GCCTTCGAGT CGTTCTTGCT CTTCGAGGGC GAGAAGAAGA 121 TCACCATTAA CAAGGACACC AAGGTACCCA ATGCCTGTTT ATTCACCATC AACAAAGAAG 181 ACCACACACT GGGAAACATC ATTAAATCAC AACTCCTAAA AGACCCGCAA GTGCTATTTG 241 CTGGCTACAA AGTCCCCCAC CCCTTGGAGC ACAAGATCAT CATCCGAGTG CAGACCACGC 301 CGGACTACAG CCCCCAGGAA GCCTTTACCA ACGCCATCAC CGACCTCATC AGTGAGCTGT 361 CCCTGCTGGA GGAGCGCTTT CGGGTGGCCA TAAAAGACAA GCAGGAAGGA ATTGAGTACC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GAACAATCGT GAGTCTTCAT TCAACTACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt