Construct: ORF TRCN0000474679
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009022.1_s317c1
- Derived from:
- ccsbBroadEn_03390
- DNA Barcode:
- TTTAATTCCTGACGCTCAGATTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLIC5 (53405)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474679
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53405 | CLIC5 | chloride intracellular chan... | NM_016929.5 | 100% | 100% | |
| 2 | human | 53405 | CLIC5 | chloride intracellular chan... | NM_001370649.1 | 84.4% | 84.4% | 0_1ins117 |
| 3 | human | 53405 | CLIC5 | chloride intracellular chan... | NM_001256023.1 | 79.6% | 75.9% | (many diffs) |
| 4 | human | 53405 | CLIC5 | chloride intracellular chan... | NM_001114086.2 | 58.7% | 58.2% | (many diffs) |
| 5 | human | 53405 | CLIC5 | chloride intracellular chan... | NM_001370650.1 | 58.7% | 58.2% | (many diffs) |
| 6 | human | 53405 | CLIC5 | chloride intracellular chan... | XM_011514692.3 | 47.2% | 45.8% | (many diffs) |
| 7 | human | 53405 | CLIC5 | chloride intracellular chan... | XM_011514694.3 | 36.7% | 30.7% | (many diffs) |
| 8 | human | 53405 | CLIC5 | chloride intracellular chan... | XR_926258.3 | 15.2% | (many diffs) | |
| 9 | human | 53405 | CLIC5 | chloride intracellular chan... | NR_045672.1 | 9.4% | (many diffs) | |
| 10 | mouse | 224796 | Clic5 | chloride intracellular chan... | NM_172621.2 | 90% | 96.4% | (many diffs) |
| 11 | mouse | 224796 | Clic5 | chloride intracellular chan... | XM_006524135.3 | 45.5% | 47.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 819
- ORF length:
- 753
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agactcggcg acagctaacg gggacgacag ggaccccgag atcgagctct 121 ttgtgaaggc tggaatcgat ggagaaagca tcggcaactg tcctttctct cagcgcctct 181 tcatgatcct ctggctgaaa ggagtcgtgt tcaatgtcac cactgtggat ctgaaaagaa 241 agccagctga cctgcacaac ctagcccccg gcacgcaccc gcccttcctg accttcaacg 301 gggacgtgaa gacagacgtc aataagatcg aggagttcct ggaggagacc ttgacccctg 361 aaaagtaccc caaactggct gcaaaacacc gggaatccaa cacagcgggc atcgacatct 421 tttccaagtt ttctgcctac atcaaaaata ccaagcagca gaacaatgct gctcttgaaa 481 gaggcctaac caaggctcta aagaaattgg atgactacct gaacacccct ctaccagagg 541 agattgacgc caacacttgt ggggaagaca aggggtcccg gcgcaagttc ctggatgggg 601 atgagctgac cctggctgac tgcaatctgt tgcccaagct ccatgtggtc aagattgtgg 661 ccaagaaata ccgcaacTAT GATATCCCGG CTGAGATGAC AGGCCTGTGG CGGTACCTCA 721 AGAACGCCTA TGCCCGTGAT GAGTTCACCA ACACCTGTGC AGCTGACAGT GAGATCGAGT 781 TGGCCTACGC TGATGTCGCC AAACGCCTCA GCCGATCCTG CCCAACTTTC TTGTACAAAG 841 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 901 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 961 ACGATTTAAT TCCTGACGCT CAGATTGCAC GCGTTAAGTC gacaatcaac ctctggatta 1021 caaaatttgt gaaagatt