Construct: ORF TRCN0000474705
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013163.1_s317c1
- Derived from:
- ccsbBroadEn_05294
- DNA Barcode:
- AACACTTAGAATGTCGTCGGGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCTD13 (253980)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474705
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253980 | KCTD13 | potassium channel tetrameri... | NM_178863.5 | 99.8% | 100% | 666C>T |
| 2 | human | 253980 | KCTD13 | potassium channel tetrameri... | XM_011545783.3 | 94.7% | 94.8% | 411_464del;720C>T |
| 3 | human | 253980 | KCTD13 | potassium channel tetrameri... | XR_001751888.2 | 78% | (many diffs) | |
| 4 | human | 253980 | KCTD13 | potassium channel tetrameri... | XM_011545784.3 | 60.1% | 59.5% | (many diffs) |
| 5 | human | 253980 | KCTD13 | potassium channel tetrameri... | XR_001751887.2 | 54.7% | (many diffs) | |
| 6 | human | 253980 | KCTD13 | potassium channel tetrameri... | XM_017023104.2 | 54% | 54.1% | 0_1ins453;213C>T |
| 7 | human | 253980 | KCTD13 | potassium channel tetrameri... | XR_950767.3 | 49.4% | (many diffs) | |
| 8 | human | 253980 | KCTD13 | potassium channel tetrameri... | XM_017023105.2 | 49.2% | 42.4% | (many diffs) |
| 9 | human | 253980 | KCTD13 | potassium channel tetrameri... | XM_017023106.2 | 47.7% | 43.6% | (many diffs) |
| 10 | human | 253980 | KCTD13 | potassium channel tetrameri... | NR_110933.2 | 43.7% | (many diffs) | |
| 11 | mouse | 233877 | Kctd13 | potassium channel tetrameri... | NM_172747.2 | 88.7% | 94.2% | (many diffs) |
| 12 | mouse | 233877 | Kctd13 | potassium channel tetrameri... | XM_006507735.3 | 71.3% | 70.9% | (many diffs) |
| 13 | mouse | 233877 | Kctd13 | potassium channel tetrameri... | XM_006507736.3 | 71.3% | 70.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1053
- ORF length:
- 987
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggcggaggcc tcgggcccgg ctgccgccgc ggccccgtcc ctggaagccc 121 ccaagccctc gggtctcgag cctggccccg ccgcctacgg tctcaagccg ctgaccccga 181 acagcaaata cgtgaagctg aacgtgggcg gctcgttgca ctacaccacg ctgcgcaccc 241 tcacgggaca ggacaccatg ctcaaagcca tgttcagcgg ccgcgtggag gtgctgaccg 301 atgccggagg ttgggtgctg attgaccgga gcggccgtca ctttggtaca atcctcaatt 361 acctgcggga tgggtctgtg ccactgccgg agagtacgag agaactgggg gagctgctgg 421 gcgaagcacg ctactacctg gtgcagggcc tgattgagga ctgccagctg gcgctgcagc 481 aaaaaaggga gacgctgtcc ccgctgtgcc tcatccccat ggtgacatct ccccgggagg 541 agcagcagct cctggccagc acctccaagc ccgtggtgaa gctcctgcac aaccgcagta 601 acaacaagta ctcctacacc agcacttcag atgacaacct acttaagaac atcgagctgt 661 tcgacaagct ggcccTGCGC TTCCACGGGC GGCTACTCTT CCTCAAGGAT GTCCTGGGGG 721 ACGAGATCTG TTGCTGGTCT TTCTACGGGC AGGGCCGCAA AATCGCCGAG GTGTGCTGCA 781 CCTCCATTGT CTATGCTACG GAGAAGAAGC AGACCAAGGT GGAATTTCCA GAGGCCCGGA 841 TCTTCGAGGA GACCCTGAAC ATCCTCATCT ACGAGACTCC CCGGGGCCCA GACCCAGCCC 901 TCCTGGAGGC CACAGGGGGA GCAGCTGGAG CTGGTGGGGC TGGCCGCGGG GAGGATGAAG 961 AGAACCGAGA GCACCGTGTC CGCAGGATCC ATGTCCGGCG CCATATCACC CACGACGAGC 1021 GTCCTCATGG CCAACAAATT GTCTTCAAGG ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1081 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1141 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 1201 CACTTAGAAT GTCGTCGGGT GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1261 ttgtgaaaga tt