Construct: ORF TRCN0000474713
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018896.3_s317c1
- Derived from:
- ccsbBroadEn_15038
- DNA Barcode:
- AATAACAGTGTAGTAGCCATAGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RPS6KC1 (26750)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474713
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_012424.6 | 99% | 20.5% | (many diffs) |
| 2 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001136138.3 | 97.9% | 19.4% | (many diffs) |
| 3 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349646.1 | 96.1% | 21.1% | (many diffs) |
| 4 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349647.1 | 88.5% | 18.2% | (many diffs) |
| 5 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001287221.2 | 82.1% | 3.5% | (many diffs) |
| 6 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349648.1 | 82.1% | 3.5% | (many diffs) |
| 7 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349649.1 | 82.1% | 3.5% | (many diffs) |
| 8 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349650.1 | 82.1% | 3.5% | (many diffs) |
| 9 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349651.1 | 82.1% | 3.5% | (many diffs) |
| 10 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349652.1 | 82.1% | 3.5% | (many diffs) |
| 11 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349653.1 | 82.1% | 3.5% | (many diffs) |
| 12 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001287218.2 | 79.2% | 3.6% | (many diffs) |
| 13 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001287219.2 | 79.2% | 3.6% | (many diffs) |
| 14 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349654.1 | 79.2% | 3.6% | (many diffs) |
| 15 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | XM_017001024.1 | 78.8% | 3.6% | (many diffs) |
| 16 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349657.1 | 72% | 6.3% | (many diffs) |
| 17 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349658.1 | 72% | 6.3% | (many diffs) |
| 18 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349659.1 | 66.8% | 6.8% | (many diffs) |
| 19 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349660.1 | 66.8% | 6.8% | (many diffs) |
| 20 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349661.1 | 66.8% | 6.8% | (many diffs) |
| 21 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349662.1 | 66.8% | 6.8% | (many diffs) |
| 22 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | XM_017001030.1 | 66.8% | 6.8% | (many diffs) |
| 23 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001287220.2 | 56.2% | 8% | (many diffs) |
| 24 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349663.1 | 56.2% | 8% | (many diffs) |
| 25 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349664.1 | 56.2% | 8% | (many diffs) |
| 26 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349665.1 | 56.2% | 8% | (many diffs) |
| 27 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349666.1 | 56.2% | 8% | (many diffs) |
| 28 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349667.1 | 56.2% | 8% | (many diffs) |
| 29 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349668.1 | 56.2% | 8% | (many diffs) |
| 30 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349669.1 | 56.2% | 8% | (many diffs) |
| 31 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349670.1 | 56.2% | 8% | (many diffs) |
| 32 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349671.1 | 56.2% | 8% | (many diffs) |
| 33 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NR_146207.1 | 54.8% | (many diffs) | |
| 34 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NR_146210.1 | 53.7% | (many diffs) | |
| 35 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NM_001349672.1 | 53.4% | 8.3% | (many diffs) |
| 36 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NR_146209.1 | 51.7% | (many diffs) | |
| 37 | human | 26750 | RPS6KC1 | ribosomal protein S6 kinase C1 | NR_146208.1 | 46.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 756
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacctcttac cgggagcgga gtgccgacct ggcccgtttc tacactgtca 121 ccgagcccca gcgacacccg aggggctaca cagtatataa ggtcaccgcc cgggttgttt 181 cacgaagaaa tccagaggat gtccaggaga taattgtatg gaagagatac agtgatttta 241 agaaactaca caaagaacta tggcaaattc acaaaaactt attccgacat tcagagttgt 301 ttcctccatt tgctaaagga atagtgtttg ggcgatttga tgaaactgtt atcgaagaga 361 gaagacaatg tgctgaagac ctgctacagt tctctgccaa tattcctgct ctttacaata 421 gtaaacagct tgaagacttt ttcaagggtg gaataattaa tgatagttct gaattaattg 481 gtcctgctga agctcactca gattccctca ttgatacctt tcctgagtgt agtacggaag 541 gcttctccag tgacagtgat ctggtatctc ttactgttga tgtggattct cttgctgagt 601 tagatgatgg aatggcttcc aatcaaaatt ctcccattag aacttttggt ctcaatcttt 661 cttcggattc ttcagcacta ggggctgttg cttctgacag tgaacagagc aaaacagaag 721 agaacgggaa agtcgtagcc tctttcctgg cagtttaaag ccgaagcttg cagagagatt 781 atttggagaa agcagagaat taataagctg gctttaaaaa gagagagacg actatgagct 841 gcttctgatt tttataggaa ggagttgatt tactcctaga aggtgttcaa ggagagtcaa 901 gccctacccg tcgagaagct gtgaagagaa gaacagccga gtacctcatg cgggcagaaa 961 gtatctctag tctttatggg aaacctcagc ttgatgatgt atctcagcct ccaggatcac 1021 taagttcaag gcccctttgg aacctaagga gccctgccga ggagctgaag gccttcagag 1081 tccttggggt gattgacaag gttttacttg taatggacac aaggacagaa cagactttca 1141 ttttaaaagg tctaaggaaa agcagtgaat acagcaggaa cagaaagacc atcatccccc 1201 gctgtgtgcc caacatggtg tgtctgcata agtacatcat ctctgaggag tcagtatttc 1261 ttgtgctgca gcatgcggaa ggtggcaaac tgtggtcata tatcagtaaa tttctaaaca 1321 gaagtcctga agaaagcttt gacatcaagg aagtgaaaaa acctacactt gcaaaagttc 1381 acctgcagca gccaacttct agtcctcagg acagcagtag ctttgaatcc agaggaagtg 1441 atggtggaag catgcttaaa gctctgcctt tgaagagtag tcttactcca agttctcaag 1501 atgacagcaa ccaggaagat gatggccaag atagctctcc aaagtggcca gattctggtt 1561 caagttcaga agaagaatgt actactagtt atttaacatt atgcaatgaa tatgggcaag 1621 aaaagattga accagggtct ttgaatgagg agcccttcat gaagactgaa gggaatggtg 1681 ttgatacaaa agctattaaa agcttcccag cacaccttgc tgctgacagt gacagcccca 1741 gcacacagct gagagctcac gagctgaagt tcttccccaa cgatgaccca gaagcagtta 1801 gttctccaag aacatcagat tccctcagta gatcaaaaaa tagccccatg gaattcttta 1861 ggatagacag taaggatagc gcaagtgaac tcctgggact tgactttgga gaaaaattgt 1921 atagtctaaa atcagaacct ttgaaaccat tctttactct tccagatgga gacagtgctt 1981 ctaggagttt taatactagt gaaagcaagg tagagtttaa agctcaggac accattagca 2041 ggggctcaga tgactcagtg ccagttattt catttaaaga tgctgctttt gatgatgtca 2101 gtggtactga tgaaggaaga cctgatcttc ttgtaaattt acctggtgaa ttggagtcaa 2161 caagagaagc tgcagcaatg ggacctacta agtttacaca aactaatata gggataatag 2221 aaaataaact cttggaagcc cctgatgttt tatgcctcag gcttagtact gaacaatgcc 2281 aagcacatga ggagaaaggc atagaggaac tgagtgatcc ctctgggccc aaatcctata 2341 gtataacaga gaaacactat gcacaggagg atcccaggat gttatttgta gcagctgttg 2401 atcatagtag ttcaggagat atgtctttgt tacccagctc aagatcttag ttttcaagga 2461 ctnggagtgg ttgagtcagc agtaactgca aacaacacag aagaaaagct attccgtatt 2521 gtagtccact ctcaggtgct aatgaatata tgcaaagcac agacacttta aaaacagaag 2581 aagtatgctg ttnnacagat cagactgatg atttggctaa agaggaacca acttctttat 2641 tccagagaga ctctgagact aagggtgaaa gtggtttagt gctagaagga gacaaggaaa 2701 tacatcagat ttttgaggac cttgataaaa aattagcact agcctccagg ttttacatcc 2761 cagagggctg cattcaaaga tgggcagctg aaatggtggt agcccttGAT GCTTTACATA 2821 GAGAGGGAAT TGTGTGCCGC GATTTGAACC CAAACAACAT CTTATTGAAT GATAGAGGAC 2881 ACATTCAGCT AACGTATTTT AGCAGGTGGA GTGAGGTTGA AGATTCCTGT GACAGCGATG 2941 CCATAGAGAG AATGTACTGT GCCCCAGAGG TTGGAGCAAT CACTGAAGAA ACTGAAGCCT 3001 GTGATTGGTG GAGTTTGGGT GCTGTCCTCT TTGAACTTCT CACTGGCAAG ACTCTGGTTG 3061 AATGCCATCC AGCAGGAATA AATACTCACA CTACTTTGAA CATGCCAGAA TGTGTCTCTG 3121 AAGAGGCTCG CTCACTCATT CAACAGCTCT TGCAGTTCAA TCCTCTGGAA CGACTTGGTG 3181 CTGGAGTTGC TGGTGTTGAA GATATCAAAT CTCATCCATT TTTTACCCCT GTGGATTGGG 3241 CAGAACTGAT GAGATTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3301 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3361 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AATAACAGTG TAGTAGCCAT 3421 AGATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt