Construct: ORF TRCN0000474759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015224.1_s317c1
- Derived from:
- ccsbBroadEn_00303
- DNA Barcode:
- TCATCAGAACGTTGCGGATCAGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHN1 (1123)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474759
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1123 | CHN1 | chimerin 1 | NM_001371513.1 | 100% | 100% | |
| 2 | human | 1123 | CHN1 | chimerin 1 | NM_001822.7 | 100% | 100% | |
| 3 | human | 1123 | CHN1 | chimerin 1 | NM_001025201.4 | 94.3% | 94.3% | 548_549ins78 |
| 4 | human | 1123 | CHN1 | chimerin 1 | NM_001371514.1 | 89% | 90.4% | 58_59ins56;91_197del |
| 5 | human | 1123 | CHN1 | chimerin 1 | NM_001206602.2 | 68.6% | 59.8% | (many diffs) |
| 6 | human | 1123 | CHN1 | chimerin 1 | NR_038133.2 | 46.1% | (many diffs) | |
| 7 | mouse | 108699 | Chn1 | chimerin 1 | NM_001113246.2 | 92.2% | 96.2% | (many diffs) |
| 8 | mouse | 108699 | Chn1 | chimerin 1 | NM_001166603.1 | 80.7% | 83.8% | (many diffs) |
| 9 | mouse | 108699 | Chn1 | chimerin 1 | XM_006498557.2 | 70.2% | 73.5% | (many diffs) |
| 10 | mouse | 108699 | Chn1 | chimerin 1 | XM_006498558.2 | 68.5% | 71.9% | (many diffs) |
| 11 | mouse | 108699 | Chn1 | chimerin 1 | NM_175752.3 | 64.1% | 56.3% | (many diffs) |
| 12 | mouse | 108699 | Chn1 | chimerin 1 | XM_006498560.3 | 53% | 53.4% | (many diffs) |
| 13 | mouse | 108699 | Chn1 | chimerin 1 | NM_001166604.1 | 52.7% | 44.8% | (many diffs) |
| 14 | mouse | 108699 | Chn1 | chimerin 1 | XM_006498561.2 | 46.1% | 49.8% | (many diffs) |
| 15 | mouse | 108699 | Chn1 | chimerin 1 | NM_029716.3 | 41.1% | 44.6% | (many diffs) |
| 16 | mouse | 108699 | Chn1 | chimerin 1 | XM_017314894.1 | 31.8% | 30.4% | (many diffs) |
| 17 | mouse | 108699 | Chn1 | chimerin 1 | XM_017314895.1 | 31.8% | 30.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1443
- ORF length:
- 1377
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctgaccctg tttgatacag atgaatatag acctcctgtt tggaaatctt 121 acttatatca gctacaacag gaagcccctc atcctcgaag aattacctgt acttgcgagg 181 tggaaaacag accaaagtat tatggaagag agtttcatgg catgatctcc agagaagcag 241 ccgaccagct cttgattgtg gctgagggga gctacctcat ccgggagagc cagcggcagc 301 cagggaccta cactttggct ttaagatttg gaagtcaaac cagaaacttc aggctctact 361 acgatggcaa gcactttgtt ggggagaaac gctttgagtc catccacgat ctggtgactg 421 atggcttgat tactctctat attgaaacca aggcagcaga atacattgcc aagatgacga 481 taaacccaat ttatgagcac gtaggataca caaccttaaa cagagagcca gcatacaaaa 541 aacatatgcc agtcctgaaa gagacacatg atgagagaga ttctacaggc caggatgggg 601 tgtcagagaa aaggttgaca tcacttgtta gaagagcaac tctgaaagaa aacgagcaaa 661 ttccaaaata tgaaaagatt cacaatttca aggtgcatac attcagaggg ccacactggt 721 gtgaatactg tgccaacttt atgtggggtc tcattgctca gggagtgaaa tgtgcagatt 781 gtggtttgaa tgttcataag cagtgttcca agatggtccc aaatgactgt aagccagact 841 tgaagcatgt caaaaaggtg tacagctgtg accttacgac gctcgtgaaa gcacatacca 901 ctaagcggcc aatggtggta gacatgtgca tcagggagat tgagtctaga ggtcttaatt 961 ctgaaggact ataccgagta tcaggattta gtgacctaat tgaagatgtc aagatggctt 1021 tcgacagaga tggtgagaag gcagatattt ctgtgaacat gtatgaagat atcaacatta 1081 tcactggtgc acttaaactg tacttcaggg atttgccaat tccactcatt acatatgatg 1141 cctacccTAA GTTTATAGAA TCTGCCAAAA TTATGGATCC GGATGAGCAA TTGGAAACCC 1201 TTCATGAAGC ACTGAAACTA CTGCCACCTG CTCACTGCGA AACCCTCCGG TACCTCATGG 1261 CACATCTAAA GAGAGTGACC CTCCACGAAA AGGAGAATCT TATGAATGCA GAGAACCTTG 1321 GAATCGTCTT TGGACCCACC CTTATGAGAT CTCCAGAACT AGACGCCATG GCTGCATTGA 1381 ATGATATACG GTATCAGAGA CTGGTGGTGG AGCTGCTTAT CAAAAACGAA GACATTTTAT 1441 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1501 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1561 GGCTTTATAT ATCTTGTGGA AAGGACGATC ATCAGAACGT TGCGGATCAG GGACGCGTTA 1621 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt