Construct: ORF TRCN0000474759

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015224.1_s317c1
Derived from:
ccsbBroadEn_00303
DNA Barcode:
TCATCAGAACGTTGCGGATCAGGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHN1 (1123)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474759
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1123 CHN1 chimerin 1 NM_001371513.1 100% 100%
2 human 1123 CHN1 chimerin 1 NM_001822.7 100% 100%
3 human 1123 CHN1 chimerin 1 NM_001025201.4 94.3% 94.3% 548_549ins78
4 human 1123 CHN1 chimerin 1 NM_001371514.1 89% 90.4% 58_59ins56;91_197del
5 human 1123 CHN1 chimerin 1 NM_001206602.2 68.6% 59.8% (many diffs)
6 human 1123 CHN1 chimerin 1 NR_038133.2 46.1% (many diffs)
7 mouse 108699 Chn1 chimerin 1 NM_001113246.2 92.2% 96.2% (many diffs)
8 mouse 108699 Chn1 chimerin 1 NM_001166603.1 80.7% 83.8% (many diffs)
9 mouse 108699 Chn1 chimerin 1 XM_006498557.2 70.2% 73.5% (many diffs)
10 mouse 108699 Chn1 chimerin 1 XM_006498558.2 68.5% 71.9% (many diffs)
11 mouse 108699 Chn1 chimerin 1 NM_175752.3 64.1% 56.3% (many diffs)
12 mouse 108699 Chn1 chimerin 1 XM_006498560.3 53% 53.4% (many diffs)
13 mouse 108699 Chn1 chimerin 1 NM_001166604.1 52.7% 44.8% (many diffs)
14 mouse 108699 Chn1 chimerin 1 XM_006498561.2 46.1% 49.8% (many diffs)
15 mouse 108699 Chn1 chimerin 1 NM_029716.3 41.1% 44.6% (many diffs)
16 mouse 108699 Chn1 chimerin 1 XM_017314894.1 31.8% 30.4% (many diffs)
17 mouse 108699 Chn1 chimerin 1 XM_017314895.1 31.8% 30.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1443
ORF length:
1377
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cctgaccctg tttgatacag atgaatatag acctcctgtt tggaaatctt
121acttatatca gctacaacag gaagcccctc atcctcgaag aattacctgt acttgcgagg
181tggaaaacag accaaagtat tatggaagag agtttcatgg catgatctcc agagaagcag
241ccgaccagct cttgattgtg gctgagggga gctacctcat ccgggagagc cagcggcagc
301cagggaccta cactttggct ttaagatttg gaagtcaaac cagaaacttc aggctctact
361acgatggcaa gcactttgtt ggggagaaac gctttgagtc catccacgat ctggtgactg
421atggcttgat tactctctat attgaaacca aggcagcaga atacattgcc aagatgacga
481taaacccaat ttatgagcac gtaggataca caaccttaaa cagagagcca gcatacaaaa
541aacatatgcc agtcctgaaa gagacacatg atgagagaga ttctacaggc caggatgggg
601tgtcagagaa aaggttgaca tcacttgtta gaagagcaac tctgaaagaa aacgagcaaa
661ttccaaaata tgaaaagatt cacaatttca aggtgcatac attcagaggg ccacactggt
721gtgaatactg tgccaacttt atgtggggtc tcattgctca gggagtgaaa tgtgcagatt
781gtggtttgaa tgttcataag cagtgttcca agatggtccc aaatgactgt aagccagact
841tgaagcatgt caaaaaggtg tacagctgtg accttacgac gctcgtgaaa gcacatacca
901ctaagcggcc aatggtggta gacatgtgca tcagggagat tgagtctaga ggtcttaatt
961ctgaaggact ataccgagta tcaggattta gtgacctaat tgaagatgtc aagatggctt
1021tcgacagaga tggtgagaag gcagatattt ctgtgaacat gtatgaagat atcaacatta
1081tcactggtgc acttaaactg tacttcaggg atttgccaat tccactcatt acatatgatg
1141cctacccTAA GTTTATAGAA TCTGCCAAAA TTATGGATCC GGATGAGCAA TTGGAAACCC
1201TTCATGAAGC ACTGAAACTA CTGCCACCTG CTCACTGCGA AACCCTCCGG TACCTCATGG
1261CACATCTAAA GAGAGTGACC CTCCACGAAA AGGAGAATCT TATGAATGCA GAGAACCTTG
1321GAATCGTCTT TGGACCCACC CTTATGAGAT CTCCAGAACT AGACGCCATG GCTGCATTGA
1381ATGATATACG GTATCAGAGA CTGGTGGTGG AGCTGCTTAT CAAAAACGAA GACATTTTAT
1441TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1501TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1561GGCTTTATAT ATCTTGTGGA AAGGACGATC ATCAGAACGT TGCGGATCAG GGACGCGTTA
1621AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)