Construct: ORF TRCN0000474875
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002997.1_s317c1
- Derived from:
- ccsbBroadEn_03347
- DNA Barcode:
- GGAGATCAGCCTGCAATCCTGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2D4 (51619)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474875
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | NM_015983.4 | 100% | 100% | |
| 2 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_011515420.3 | 85.7% | 85% | (many diffs) |
| 3 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_006715734.3 | 85.7% | 85.3% | 441_513delinsG |
| 4 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_017012294.1 | 80.8% | 79.6% | (many diffs) |
| 5 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_017012293.1 | 80.3% | 79.2% | (many diffs) |
| 6 | human | 7322 | UBE2D2 | ubiquitin conjugating enzym... | NM_003339.3 | 78% | 93.1% | (many diffs) |
| 7 | human | 7321 | UBE2D1 | ubiquitin conjugating enzym... | NM_003338.5 | 77.5% | 91.8% | (many diffs) |
| 8 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181891.3 | 77% | 91.8% | (many diffs) |
| 9 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | XM_024454202.1 | 77% | 91.8% | (many diffs) |
| 10 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_017012292.1 | 74.9% | 73.8% | (many diffs) |
| 11 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_024446796.1 | 74.8% | 74.8% | 0_1ins111 |
| 12 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_024446795.1 | 74.7% | 73.9% | (many diffs) |
| 13 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_011515422.3 | 74.1% | 72.7% | 0_1ins82;6_7ins32 |
| 14 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_017012295.1 | 63.5% | 61.9% | 0_1ins82;6_7ins32;327_399delinsG |
| 15 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | XM_017008584.2 | 62.8% | 74.1% | (many diffs) |
| 16 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_011515423.2 | 47.2% | 46.7% | (many diffs) |
| 17 | mouse | 76508 | Ube2d-ps | ubiquitin-conjugating enzym... | NR_015577.1 | 21.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctaaagcgg atccagaagg aattaaccga cttgcagagg gatcctcctg 121 cccagtgttc tgcaggacct gtcggtgatg acttgttcca ctggcaggcc accatcatgg 181 gcccGAATGA CAGTCCTTAC CAAGGAGGTG TTTTCTTCCT GACCATCCAC TTTCCTACAG 241 ATTACCCGTT CAAGCCCCCA AAGGTTGCTT TCACAACCAA AATTTATCAC CCTAATATCA 301 ACAGCAATGG CAGCATCTGC CTTGATATCC TGCGGTCTCA GTGGTCTCCA GCGTTGACTG 361 TGTCAAAAGT TCTCTTGTCC ATCTGCTCGC TGCTCTGCGA CCCCAACCCC GATGACCCCC 421 TGGTGCCAGA GATAGCACAC ACCTACAAGG CCGACAGAGA GAAGTACAAC AGACTAGCAA 481 GAGAGTGGAC ACAAAAATAT GCTATGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGAGATCA 661 GCCTGCAATC CTGTTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt