Construct: ORF TRCN0000474897
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018952.2_s317c1
- Derived from:
- ccsbBroadEn_15142
- DNA Barcode:
- TCCGATTATTTCCCTTACGATCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- WNK4 (65266)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474897
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65266 | WNK4 | WNK lysine deficient protei... | NM_032387.5 | 77.8% | 8.8% | (many diffs) |
| 2 | human | 65266 | WNK4 | WNK lysine deficient protei... | XM_017024962.1 | 74.6% | 8.4% | (many diffs) |
| 3 | human | 65266 | WNK4 | WNK lysine deficient protei... | NM_001321299.2 | 67.9% | 4.7% | (many diffs) |
| 4 | human | 65266 | WNK4 | WNK lysine deficient protei... | XM_017024966.1 | 64.8% | 4.5% | (many diffs) |
| 5 | human | 5681 | PSKH1 | protein serine kinase H1 | NM_006742.3 | 33.6% | 5.6% | 0_1ins319;1272_1273ins2185 |
| 6 | mouse | 244631 | Pskh1 | protein serine kinase H1 | NM_173432.2 | 29.8% | 3.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttggcatcc ccggccacgg agaccaccgt cctcatgtcc cagactgagg 121 ccgacctggc cctgcggccc ccgcctcctc ttggcaccgc ggggcagccc cgcctcgggc 181 cccctcctcg ccgagcgcgc cgcttctccg ggaaggctga gccccggccg cgctcttctc 241 gtctcagccg ccgtagctca gtcgacttgg ggctgctgag ctcttggtcc ctgccagcct 301 cacccgctcc ggaccccccc gatcctccgg actccgctgg tcctggcccc gcgaggagcc 361 caccgcctag ctccaaagaa ccccccgatg ggctgtggga caagcaaggt ccttcccgag 421 ccacccaagg atgtccagct ggatctggtc aagaaggtgg agcccttcag tggcactaag 481 agtgacgtgt acaagcactt catcacagag gtggacagtg ttggccctgt caaagccggg 541 ttcccagcag caagtcagta tgcacacccc tgccccggtc ccccgactgc tggccacacg 601 gagcctccct cagaaccacc acgcagggcc agggtagcta agtacagggc caagtttgac 661 ccacgtgtta cagctaagta tgacatcaag gccctaattg gccgaggcag cttcagccga 721 gtggtacgtg tagagcaccg ggcaacccgg cagccgtatg ccatcaagat gattgagacc 781 aagtaccggg aggggcggga ggtgtgtgag tcggagctgc gtgtgctgcg tcgggtgcgt 841 catgccaaca tcatccagct ggtggaggtg ttcgagacac aggagcgggt gtacatggtg 901 atggagctgg ccactggtgg agagctcttt gaccgcatca ttgccaaggg ctccttcacc 961 gagcgtgacg ccacgcgggt gctgcagatg gtgctggatg gcgtccggta tctgcatgca 1021 ctgggcatca cacaccgaga cctcaaacct gagaatctgc tctactacca tccgggcact 1081 gactccaaga tcatcatcac cgacttcggc ctggccagtg ctcgcaagaa gggtgatgac 1141 tgcttgatga agaccacctg tggcacgcct gagtacattg ccccagaagt cctggtccgc 1201 aagccataca ccaactcagt ggacatgtgg gcgctgggcg tcattgccta catcctactc 1261 agtggcacca tgccgtttga ggatgacaac cgtacccggc tgtaccggca gatcctcagg 1321 ggcaagtaca gttactctgg ggagccctgg cctagtgtgt ccaacctggc caaggacttc 1381 attgaccgcc tgctgacagt ggaccctgga gcccgtatga ctgcactgca ggccctgagg 1441 cacccgtggg tggtgagcat ggctgcctct tcatccatga agaacctgca ccgctccata 1501 tcccagaacc tccttaaacg tgcctcctcg cgctgccaga gcaccaaatc tgcccagtcc 1561 acgcgttcca gccgctccac acgctccaat aagtcacgcc gtgtgcggga acgggagctg 1621 cgggagctca acctgcgcta ccagcagcaa tacaatggct tgctgccatc cagcgaaagc 1681 gtgagaagct gcgtaaagca agggaattgg aggcactccc accagagcca ggacctccac 1741 cagcaactgt gcccatggcc cccggtcccc ccagtgtctt cccccctgag cctgaggagc 1801 cagaggcaga ccagcaccag cccttccttt tccgccacgc cagctactca tctaccactt 1861 cggattgcga gactgatggc tacctcagct cctccggctt cctggatgcc tcagaccctg 1921 cccttcagcc ccctgggggg gtgccatcca gcctggctga gtcccatctc tgcctgccct 1981 cggcttttgc cctatccatt ccacgttctg gccctggaag tgacttttcc cccggggaca 2041 gctatgcctc agatgcagct tcaggcctta gcgatgtggg agaagggatg ggacaaatga 2101 ggagaccccc agggaggaat ctccggcgca gaccccgatc ccggctgcgg gtcactagtg 2161 tctcagacca gaatgacaga gtggttgagt gccagctaca gacccataac agcaagatgg 2221 tgaccttccg atttgatctg gatggggaca gcccggaaga gattgcagct gccatggtat 2281 ataacgagtt cattctgcct tcggagcgag atggatttct cagacggatt cgggagatta 2341 tccagcgagt ggagaccctg ttgaagagag acactggccc catggaggct gctgaagaca 2401 ccctaagccc ccaggaggag ccagcaccat tacctgccct gcccgtcccc ctcccagacc 2461 catccaatga agagctccag agcagcacct ccctggagca caggagctgg acagccttct 2521 ccacctcctc atcttctcct ggaactcctt tgtctcctgg aaacccattt tcccctggaa 2581 cccccatttc cccaggtccc atcttcccca tcacttctcc cccatgtcat cccagcccct 2641 ccccattctc ccccatttct tcccaggtct cctcaaatcc ctctccacac cccaccagct 2701 ctccacttcc attctcctcc agcacacccg agtttccggt cccactctct cagtgtccct 2761 ggagttctct ccccacgact tctccaccta cgttctctcc cacttgttct caggtcactc 2821 ttagttcccc tttctttcct ccgtgcccct ccacttcttc cttcccctcc accacagcag 2881 cccctctcct ttctctggct agtgccttct cactggctgt gatgactgtg gcccagtccc 2941 tgctgtcccc ctcacctggg ctcctttccc agtctcctcc agcccctcct agtcccctcc 3001 ctagcctgcc ccttccccct cccgttgctc ctggtggcca ggaaagccct tcaccccaca 3061 cagctgaggt ggagagtgag gcctccacca cctctgctcg cccctcccag gggaagcagg 3121 ctggcgccca tctctgaaga gggaaagccg cagcttgtgg gcgtttccaa gtgacttcat 3181 ccaaggaact ggctgagcct cttccttgca gccaacatcc cccactctct ctggttctcc 3241 aaaacattca acccctcagc tcacttcaga gagctcagat acagaggaca gtgctggagg 3301 cgggccagag accagggaag ctctggctga gagcgaccgt gcagctgagg gtctgggggc 3361 tggagttgag gaggaaggag atgatgggaa ggaaccccaa gttgggggca gcccccaacc 3421 cctGAGCCAT CCCAGCCCAG TGTGGATGAA CTACTCCTAC AGCAGCCTGT GTTTGAGCAG 3481 CGAGGAGTCA GAAAGCAGTG GGGAAGATGA GGAGTTCTGG GCTGAGCTGC AGAGTCTTCG 3541 GCAGAAGCAC TTGTCAGAGG TGGAAACACT ACAGACACTA CAGAAAAAAG AAATTGAAGA 3601 TTTGTACAGC CGGCTGGGGA AGCAGCCCCC ACCGGGTATT GTGGCCCCAG CTGCTATGCT 3661 GTCCAGCCGC CAGCGCCGCC TCTCCAAGGG CAGCTTCCCC ACCTCCCGCC GCAACAGCCT 3721 ACAGCGCTCT GAGCCCCCAG GCCCTGGCAT CATGCGAAGG AACTCTCTGA GTGGCAGCAG 3781 CACCGGCTCC CAGGAGCAGC GGGCAAGCAA GGGGGTGACA TTCGCCGGGG ATGTTGGCAG 3841 GATGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 3901 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 3961 TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCCGATTATT TCCCTTACGA TCAAACGCGT 4021 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt