Construct: ORF TRCN0000474898
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018887.3_s317c1
- Derived from:
- ccsbBroadEn_14616
- DNA Barcode:
- GCATCTGGCTCATCTTTTACCAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DLG3 (1741)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474898
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | NM_020730.3 | 97.6% | 43.9% | (many diffs) |
| 2 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029322.2 | 94.9% | 45.1% | (many diffs) |
| 3 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029323.2 | 93.7% | 45.5% | (many diffs) |
| 4 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029324.2 | 91% | 46.8% | (many diffs) |
| 5 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029325.2 | 72.4% | 18.3% | (many diffs) |
| 6 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | NM_001166278.1 | 69.4% | 15.4% | (many diffs) |
| 7 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_005262248.4 | 69.4% | 15.4% | (many diffs) |
| 8 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029326.2 | 69.4% | 15.4% | (many diffs) |
| 9 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_017029327.1 | 62.8% | 16.4% | (many diffs) |
| 10 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_006724625.2 | 57.3% | 22.2% | (many diffs) |
| 11 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_011530883.1 | 55.7% | 22.6% | (many diffs) |
| 12 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | XM_006724626.2 | 54.9% | 22.7% | (many diffs) |
| 13 | human | 1741 | DLG3 | discs large MAGUK scaffold ... | NM_021120.4 | 53.3% | 23.1% | (many diffs) |
| 14 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_001177779.1 | 91.5% | 43.3% | (many diffs) |
| 15 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_001177780.1 | 65.7% | 15.4% | (many diffs) |
| 16 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | XM_006528127.3 | 65.7% | 15.4% | (many diffs) |
| 17 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | XM_006528124.3 | 54.6% | 22.2% | (many diffs) |
| 18 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | XM_006528123.3 | 53.5% | 21.7% | (many diffs) |
| 19 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_016747.4 | 51.3% | 22.2% | (many diffs) |
| 20 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_001290402.1 | 50.8% | 23.1% | (many diffs) |
| 21 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | NM_001177778.2 | 49.7% | 22.6% | (many diffs) |
| 22 | mouse | 53310 | Dlg3 | discs, large homolog 3 (Dro... | XR_001782754.1 | 24.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 762
- ORF length:
- 693
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagagggcc cgcaagttct cgggctccgg cttggccatg ggcttgggct 121 ccgcctccgc ttcggcctgg aggagggctt cgcagaggtg ggcctggccg ctccgctccc 181 tgcggcccgg aggggatgcc agagagcctc gcaagatcat cctgcacaaa ggctccacag 241 gcctgggctt caacatcgta ggaggagagg atggagaagg catttttgtc tccttcatcc 301 tggcaggagg cccagctgac ctgagtgggg agctgcgcag gggagaccgg atcttatcgg 361 tgaatggagt gaatctgagg aatgcaactc atgagcaggc tgcagctgct ctgaaacggg 421 ccggccagtc agtcaccatt gtggcccaat acagacctga agaatacagt cgctttgaat 481 cgaagataca tgacttacga gaacaaatga tgaacagcag catgagctct gggtctgggt 541 ccctccgaac aagtgagaag aggtccttgt atgtcacggc cctgtttgat tatgatcgga 601 ctctggacag ctgcctgcca agccaggggc tcagcttctc ttatggtgac attctgcatg 661 tcattaatgc ctctgatgat gagtggtggc aggcaatgct ggtgacccca cacagagaaa 721 gtgagcatat cggtgtgatc cccagtaaga gcagggtgga atagaaataa ccagcttcga 781 tacaaactgt gaactttcat gccaggacgg ggatggagtg agtccaatgc agttctgatc 841 agaatcccag acgtagnaag agtttcggcc tctctcgaaa gtttccattt tacaagagca 901 aagaaaacat ggcccaggag agcagcatac aggaacaggg gagtgacatc caacacccag 961 tgacagcgaa agcagttcca aaggacaaga ggatgctatt ttgtcatatg agccagtgac 1021 acggcaagaa attcactatg caaggcctgt gatcatcctg ggcccaatga aggaccgagt 1081 caatgatgac ctgatctccg aatttccaca taaatttgga tcctgtgtgc cacatactac 1141 ccggccTCGA CGTGATAATG AGGTGGATGG ACAAGACTAC CACTTTGTGG TGTCCCGAGA 1201 ACAAATGGAG AAAGATATTC AGGACAACAA GTTCATCGAG GCGGGCCAAT TTAATGATAA 1261 CCTCTATGGG ACCAGCATCC AGTCAGTGCG GGCAGTTGCA GAGAGGGGCA AGCACTGCAT 1321 CTTAGATGTT TCCGGCAATG CTATCAAGAG ACTGCAGCAA GCACAACTTT ACCCCATTGC 1381 CATTTTCATC AAGCCCAAGT CCATTGAAGC CCTTATGGAA ATGAACCGAA GGCAGACATA 1441 TGAACAAGCA AATAAGATCT ATGACAAAGC CATGAAACTG GAGCAGGAAT TTGGAGAGTA 1501 CTTTACAGCC ATTGTACAGG GTGACTCACT GGAAGAGATT TATAACAAAA TCAAACAAAT 1561 CATTGAGGAC CAGTCTGGGC ACTACATTTG GGTCCCATCC CCTGAAAAAC TCTTGCCAAC 1621 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 1681 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 1741 ATCTTGTGGA AAGGACGAGC ATCTGGCTCA TCTTTTACCA ATACGCGTTA AGTCgacaat 1801 caacctctgg attacaaaat ttgtgaaaga tt