Construct: ORF TRCN0000474898

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018887.3_s317c1
Derived from:
ccsbBroadEn_14616
DNA Barcode:
GCATCTGGCTCATCTTTTACCAAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DLG3 (1741)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474898
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1741 DLG3 discs large MAGUK scaffold ... NM_020730.3 97.6% 43.9% (many diffs)
2 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029322.2 94.9% 45.1% (many diffs)
3 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029323.2 93.7% 45.5% (many diffs)
4 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029324.2 91% 46.8% (many diffs)
5 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029325.2 72.4% 18.3% (many diffs)
6 human 1741 DLG3 discs large MAGUK scaffold ... NM_001166278.1 69.4% 15.4% (many diffs)
7 human 1741 DLG3 discs large MAGUK scaffold ... XM_005262248.4 69.4% 15.4% (many diffs)
8 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029326.2 69.4% 15.4% (many diffs)
9 human 1741 DLG3 discs large MAGUK scaffold ... XM_017029327.1 62.8% 16.4% (many diffs)
10 human 1741 DLG3 discs large MAGUK scaffold ... XM_006724625.2 57.3% 22.2% (many diffs)
11 human 1741 DLG3 discs large MAGUK scaffold ... XM_011530883.1 55.7% 22.6% (many diffs)
12 human 1741 DLG3 discs large MAGUK scaffold ... XM_006724626.2 54.9% 22.7% (many diffs)
13 human 1741 DLG3 discs large MAGUK scaffold ... NM_021120.4 53.3% 23.1% (many diffs)
14 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_001177779.1 91.5% 43.3% (many diffs)
15 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_001177780.1 65.7% 15.4% (many diffs)
16 mouse 53310 Dlg3 discs, large homolog 3 (Dro... XM_006528127.3 65.7% 15.4% (many diffs)
17 mouse 53310 Dlg3 discs, large homolog 3 (Dro... XM_006528124.3 54.6% 22.2% (many diffs)
18 mouse 53310 Dlg3 discs, large homolog 3 (Dro... XM_006528123.3 53.5% 21.7% (many diffs)
19 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_016747.4 51.3% 22.2% (many diffs)
20 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_001290402.1 50.8% 23.1% (many diffs)
21 mouse 53310 Dlg3 discs, large homolog 3 (Dro... NM_001177778.2 49.7% 22.6% (many diffs)
22 mouse 53310 Dlg3 discs, large homolog 3 (Dro... XR_001782754.1 24.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
762
ORF length:
693
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagagggcc cgcaagttct cgggctccgg cttggccatg ggcttgggct
121ccgcctccgc ttcggcctgg aggagggctt cgcagaggtg ggcctggccg ctccgctccc
181tgcggcccgg aggggatgcc agagagcctc gcaagatcat cctgcacaaa ggctccacag
241gcctgggctt caacatcgta ggaggagagg atggagaagg catttttgtc tccttcatcc
301tggcaggagg cccagctgac ctgagtgggg agctgcgcag gggagaccgg atcttatcgg
361tgaatggagt gaatctgagg aatgcaactc atgagcaggc tgcagctgct ctgaaacggg
421ccggccagtc agtcaccatt gtggcccaat acagacctga agaatacagt cgctttgaat
481cgaagataca tgacttacga gaacaaatga tgaacagcag catgagctct gggtctgggt
541ccctccgaac aagtgagaag aggtccttgt atgtcacggc cctgtttgat tatgatcgga
601ctctggacag ctgcctgcca agccaggggc tcagcttctc ttatggtgac attctgcatg
661tcattaatgc ctctgatgat gagtggtggc aggcaatgct ggtgacccca cacagagaaa
721gtgagcatat cggtgtgatc cccagtaaga gcagggtgga atagaaataa ccagcttcga
781tacaaactgt gaactttcat gccaggacgg ggatggagtg agtccaatgc agttctgatc
841agaatcccag acgtagnaag agtttcggcc tctctcgaaa gtttccattt tacaagagca
901aagaaaacat ggcccaggag agcagcatac aggaacaggg gagtgacatc caacacccag
961tgacagcgaa agcagttcca aaggacaaga ggatgctatt ttgtcatatg agccagtgac
1021acggcaagaa attcactatg caaggcctgt gatcatcctg ggcccaatga aggaccgagt
1081caatgatgac ctgatctccg aatttccaca taaatttgga tcctgtgtgc cacatactac
1141ccggccTCGA CGTGATAATG AGGTGGATGG ACAAGACTAC CACTTTGTGG TGTCCCGAGA
1201ACAAATGGAG AAAGATATTC AGGACAACAA GTTCATCGAG GCGGGCCAAT TTAATGATAA
1261CCTCTATGGG ACCAGCATCC AGTCAGTGCG GGCAGTTGCA GAGAGGGGCA AGCACTGCAT
1321CTTAGATGTT TCCGGCAATG CTATCAAGAG ACTGCAGCAA GCACAACTTT ACCCCATTGC
1381CATTTTCATC AAGCCCAAGT CCATTGAAGC CCTTATGGAA ATGAACCGAA GGCAGACATA
1441TGAACAAGCA AATAAGATCT ATGACAAAGC CATGAAACTG GAGCAGGAAT TTGGAGAGTA
1501CTTTACAGCC ATTGTACAGG GTGACTCACT GGAAGAGATT TATAACAAAA TCAAACAAAT
1561CATTGAGGAC CAGTCTGGGC ACTACATTTG GGTCCCATCC CCTGAAAAAC TCTTGCCAAC
1621TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA
1681TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT
1741ATCTTGTGGA AAGGACGAGC ATCTGGCTCA TCTTTTACCA ATACGCGTTA AGTCgacaat
1801caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.