Construct: ORF TRCN0000474918
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005666.1_s317c1
- Derived from:
- ccsbBroadEn_15942
- DNA Barcode:
- GTACTAACTACGACCGGACATTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PELI1 (57162)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474918
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532997.2 | 52.3% | 52.3% | 1_342del |
| 2 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_005264437.3 | 39% | 39% | 1_585del |
| 3 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532996.2 | 39% | 39% | 1_585del |
| 4 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_017004521.2 | 35.5% | 35.5% | 1_681del |
| 5 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | NM_020651.4 | 29.9% | 29.9% | 1_879del |
| 6 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532994.3 | 29.9% | 29.9% | 1_879del |
| 7 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_017004520.1 | 29.9% | 29.9% | 1_879del |
| 8 | mouse | 67245 | Peli1 | pellino 1 | XM_011243746.1 | 33.4% | 35.5% | (many diffs) |
| 9 | mouse | 67245 | Peli1 | pellino 1 | NM_023324.2 | 28.1% | 29.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 465
- ORF length:
- 375
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAGAGGAA AGACGTTGTA GATGAAAAAC 121 AACCATGGGT ATATCTAAAC TGCGGCCATG TACATGGCTA TCATAACTGG GGAAACAAAG 181 AAGAACGTGA TGGAAAAGAT CGTGAATGTC CTATGTGTAG GTCTGTTGGT CCCTATGTTC 241 CTCTGTGGCT TGGATGTGAA GCTGGATTTT ATGTGGACGC CGGCCCTCCA ACCCATGCGT 301 TTAGCCCGTG TGGGCATGTG TGTTCAGAAA AGACAACTGC CTATTGGTCC CAGATCCCAC 361 TTCCTCATGG TACTCATACT TTTCATGCAG CCTGTCCCTT TTGTGCACAT CAGTTGGCTG 421 GTGAACAAGG CTACATCAGA CTTATTTTTC AAGGACCTCT AGACTACCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA GTACTAACTA CGACCGGACA TTTCACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt