Construct: ORF TRCN0000474947
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015922.2_s317c1
- Derived from:
- ccsbBroadEn_14528
- DNA Barcode:
- CCGTTCGCCTTGATTCTTTCGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACVR1B (91)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474947
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91 | ACVR1B | activin A receptor type 1B | NM_004302.5 | 100% | 100% | |
| 2 | human | 91 | ACVR1B | activin A receptor type 1B | NM_020328.4 | 92.4% | 92.3% | 812_934del |
| 3 | human | 91 | ACVR1B | activin A receptor type 1B | NM_020327.3 | 89.7% | 89.7% | 0_1ins156 |
| 4 | human | 91 | ACVR1B | activin A receptor type 1B | XM_017020201.2 | 75% | 75.2% | 1137_1139delGAGinsA;1141C>A;1143_1144ins374 |
| 5 | human | 91 | ACVR1B | activin A receptor type 1B | XM_011538966.3 | 69.4% | 69.4% | (many diffs) |
| 6 | human | 91 | ACVR1B | activin A receptor type 1B | XM_011538967.3 | 59.1% | 55.5% | (many diffs) |
| 7 | mouse | 11479 | Acvr1b | activin A receptor, type 1B | NM_007395.3 | 90.8% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1581
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagtcggcc ggagcctcct ccttcttccc ccttgttgtc ctcctgctcg 121 ccggcagcgg cgggtccggg ccccgggggg tccaggctct gctgtgtgcg tgcaccagct 181 gcctccaggc caactacacg tgtgagacag atggggcctg catggtttcc attttcaatc 241 tggatgggat ggagcaccat gtgcgcacct gcatccccaa agtggagctg gtccctgccg 301 ggaagccctt ctactgcctg agctcggagg acctgcgcaa cacccactgc tgctacactg 361 actactgcaa caggatcgac ttgagggtgc ccagtggtca cctcaaggag cctgagcacc 421 cgtccatgtg gggcccggtg gagctggtag gcatcatcgc cggcccggtg ttcctcctgt 481 tcctcatcat catcattgtt ttccttgtca ttaactatca tcagcgtgtc tatcacaacc 541 gccagagact ggacatggaa gatccctcat gtgagatgtg tctctccaaa gacaagacgc 601 tccaggatct tgtctacgat ctctccacct cagggtctgg ctcagggtta cccctctttg 661 tccagcgcac agtggcccga accatcgttt tacaagagat tattggcaag ggtcggtttg 721 gggaagtatg gcggggccgc tggaggggtg gtgatgtggc tgtgaaaata ttctcttctc 781 gtgaagaacg gtcttggttc agggaagcag agatatacca gacggtcatg ctgcgccatg 841 aaaacatcct tggatttatt gctgctgaca ataaagataa tggcacctgg acacagctgt 901 ggcttgtttc tgactatcat gagcacgggt ccctgtttga ttatctgaac cggtacacag 961 tgacaattga ggggatgatt aagctggcct tgtctgctgc tagtgggctg gcacacctgc 1021 acatggagat cgtgggcacc caagggaagc ctggaattgc tcatcgagac ttaaagtcaa 1081 agaacattct ggtgaagaaa aatggcatgt gtgccatagc agacctgggc ctggctgtcc 1141 gtcatgatgc agtcactgac accattgaca ttgccccgaa tcagagggtg gggaccaaac 1201 gatacatggc ccctGAAGTA CTTGATGAAA CCATTAATAT GAAACACTTT GACTCCTTTA 1261 AATGTGCTGA TATTTATGCC CTCGGGCTTG TATATTGGGA GATTGCTCGA AGATGCAATT 1321 CTGGAGGAGT CCATGAAGAA TATCAGCTGC CATATTACGA CTTAGTGCCC TCTGACCCTT 1381 CCATTGAGGA AATGCGAAAG GTTGTATGTG ATCAGAAGCT GCGTCCCAAC ATCCCCAACT 1441 GGTGGCAGAG TTATGAGGCA CTGCGGGTGA TGGGGAAGAT GATGCGAGAG TGTTGGTATG 1501 CCAACGGCGC AGCCCGCCTG ACGGCCCTGC GCATCAAGAA GACCCTCTCC CAGCTCAGCG 1561 TGCAGGAAGA CGTGAAGATC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGT TCGCCTTGAT 1741 TCTTTCGACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt