Transcript: Human XM_017020201.2

PREDICTED: Homo sapiens activin A receptor type 1B (ACVR1B), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACVR1B (91)
Length:
1315
CDS:
49..1194

Additional Resources:

NCBI RefSeq record:
XM_017020201.2
NBCI Gene record:
ACVR1B (91)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017020201.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000226395 ACTATCATCAGCGTGTCTATC pLKO_005 497 CDS 100% 10.800 15.120 N ACVR1B n/a
2 TRCN0000195680 CTACTGCAACAGGATCGACTT pLKO.1 345 CDS 100% 4.050 5.670 N ACVR1B n/a
3 TRCN0000195389 CCGGTACACAGTGACAATTGA pLKO.1 933 CDS 100% 5.625 4.500 N ACVR1B n/a
4 TRCN0000001812 AGCAGAGATATACCAGACGGT pLKO.1 789 CDS 100% 0.660 0.528 N ACVR1B n/a
5 TRCN0000355763 ACGGGTCCCTGTTTGATTATC pLKO_005 908 CDS 100% 13.200 9.240 N ACVR1B n/a
6 TRCN0000196765 GAATTGCTCATCGAGACTTAA pLKO.1 1037 CDS 100% 13.200 9.240 N ACVR1B n/a
7 TRCN0000355762 TTCAGGGAAGCAGAGATATAC pLKO_005 781 CDS 100% 13.200 9.240 N ACVR1B n/a
8 TRCN0000010152 TGTGGCTTGTTTCTGACTATC pLKO.1 881 CDS 100% 10.800 7.560 N ACVR1B n/a
9 TRCN0000001810 CCACTGCTGCTACACTGACTA pLKO.1 327 CDS 100% 4.950 3.465 N ACVR1B n/a
10 TRCN0000010160 CCACTGCTGCTACACTGACTA pLKO.1 327 CDS 100% 4.950 3.465 N ACVR1B n/a
11 TRCN0000001813 CCTTGTCATTAACTATCATCA pLKO.1 486 CDS 100% 4.950 3.465 N ACVR1B n/a
12 TRCN0000196244 GCTGCCATATTACGACTTAGT pLKO.1 1204 3UTR 100% 4.950 3.465 N ACVR1B n/a
13 TRCN0000001811 GCTGTGGCTTGTTTCTGACTA pLKO.1 879 CDS 100% 4.950 3.465 N ACVR1B n/a
14 TRCN0000010151 GGGAAGCAGAGATATACCAGA pLKO.1 785 CDS 100% 2.640 1.848 N ACVR1B n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017020201.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00018 pDONR223 100% 75% 75.2% None 1137_1139delGAGinsA;1141C>A;1143_1144ins374 n/a
2 ccsbBroad304_00018 pLX_304 34.5% 75% 75.2% V5 1137_1139delGAGinsA;1141C>A;1143_1144ins374 n/a
3 TRCN0000465737 GGCATCCGACAGGTTCTCTTAACC pLX_317 26% 75% 75.2% V5 1137_1139delGAGinsA;1141C>A;1143_1144ins374 n/a
4 ccsbBroadEn_14528 pDONR223 0% 75% 75.2% None 1137_1139delGAGinsA;1141C>A;1143_1144ins374 n/a
5 ccsbBroad304_14528 pLX_304 26% 75% 75.2% V5 1137_1139delGAGinsA;1141C>A;1143_1144ins374 n/a
6 TRCN0000474947 CCGTTCGCCTTGATTCTTTCGACA pLX_317 24.5% 75% 75.2% V5 1137_1139delGAGinsA;1141C>A;1143_1144ins374 n/a
7 TRCN0000489615 TTGCACTTGCCTACGTGGGCGCCT pLX_317 22.2% 75% 75.2% V5 (not translated due to prior stop codon) 1137_1139delGAGinsA;1141C>A;1143_1144ins374 n/a
Download CSV