Transcript: Human XM_011538967.3

PREDICTED: Homo sapiens activin A receptor type 1B (ACVR1B), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACVR1B (91)
Length:
1631
CDS:
49..987

Additional Resources:

NCBI RefSeq record:
XM_011538967.3
NBCI Gene record:
ACVR1B (91)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144965 ACTTGACTCAGGTCACCTCA pXPR_003 AGG 337 36% 3 0.4392 ACVR1B ACVR1B 76680
2 BRDN0001146179 AAGAGATTATTGGCAAGGGT pXPR_003 CGG 645 69% 4 0.2949 ACVR1B ACVR1B 76681
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011538967.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000226395 ACTATCATCAGCGTGTCTATC pLKO_005 497 CDS 100% 10.800 15.120 N ACVR1B n/a
2 TRCN0000195680 CTACTGCAACAGGATCGACTT pLKO.1 345 CDS 100% 4.050 5.670 N ACVR1B n/a
3 TRCN0000001812 AGCAGAGATATACCAGACGGT pLKO.1 789 CDS 100% 0.660 0.528 N ACVR1B n/a
4 TRCN0000355762 TTCAGGGAAGCAGAGATATAC pLKO_005 781 CDS 100% 13.200 9.240 N ACVR1B n/a
5 TRCN0000001810 CCACTGCTGCTACACTGACTA pLKO.1 327 CDS 100% 4.950 3.465 N ACVR1B n/a
6 TRCN0000010160 CCACTGCTGCTACACTGACTA pLKO.1 327 CDS 100% 4.950 3.465 N ACVR1B n/a
7 TRCN0000001813 CCTTGTCATTAACTATCATCA pLKO.1 486 CDS 100% 4.950 3.465 N ACVR1B n/a
8 TRCN0000010151 GGGAAGCAGAGATATACCAGA pLKO.1 785 CDS 100% 2.640 1.848 N ACVR1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011538967.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00018 pDONR223 100% 59.1% 55.5% None (many diffs) n/a
2 ccsbBroad304_00018 pLX_304 34.5% 59.1% 55.5% V5 (many diffs) n/a
3 TRCN0000465737 GGCATCCGACAGGTTCTCTTAACC pLX_317 26% 59.1% 55.5% V5 (many diffs) n/a
4 ccsbBroadEn_14528 pDONR223 0% 59.1% 55.5% None (many diffs) n/a
5 ccsbBroad304_14528 pLX_304 26% 59.1% 55.5% V5 (many diffs) n/a
6 TRCN0000474947 CCGTTCGCCTTGATTCTTTCGACA pLX_317 24.5% 59.1% 55.5% V5 (many diffs) n/a
7 TRCN0000489615 TTGCACTTGCCTACGTGGGCGCCT pLX_317 22.2% 59.1% 55.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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