Construct: ORF TRCN0000475007

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018893.3_s317c1
Derived from:
ccsbBroadEn_14758
DNA Barcode:
AATCTTGGTAACTGTATGAATAAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PIK3C3 (5289)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475007
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5289 PIK3C3 phosphatidylinositol 3-kina... NM_002647.4 99.5% 21.3% (many diffs)
2 human 5289 PIK3C3 phosphatidylinositol 3-kina... NM_001308020.2 92.4% 14.2% (many diffs)
3 human 5289 PIK3C3 phosphatidylinositol 3-kina... XM_011526031.3 78.6% 5.5% (many diffs)
4 human 5289 PIK3C3 phosphatidylinositol 3-kina... XR_002958176.1 41.4% (many diffs)
5 human 5289 PIK3C3 phosphatidylinositol 3-kina... XR_935226.1 40% (many diffs)
6 human 5289 PIK3C3 phosphatidylinositol 3-kina... XR_001753204.2 39% (many diffs)
7 mouse 225326 Pik3c3 phosphatidylinositol 3-kina... NM_181414.5 87.4% 21% (many diffs)
8 mouse 225326 Pik3c3 phosphatidylinositol 3-kina... XR_385985.2 58.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
651
ORF length:
582
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggggaagca gagaagtttc actacatcta tagttgtgac ctggatatca
121acgtccagct taagatagga agcttggaag ggaagagaga acaaaagagt tataaagctg
181tcctggaaga cccaatgttg aagttctcag gactatatca agagacatgc tctgatcttt
241atgttacttg tcaagttttt gcagaaggga agcctttggc cttgccagtg agaacatcct
301acaaagcatt tagtacaaga tggaactgga atgaatggct gaaactacca gtaaaatacc
361ctgacctgcc caggaatgcc caagtggccc tcaccatatg ggatgtgtat ggtcccggaa
421aagcagtgcc tgtaggagga acaacggttt cgctctttgg aaaatacggc atgtttcgcc
481aagggatgca tgacttgaaa gtctggccta atgtagaagc agatggatca gaacccacaa
541aaactcctgg cagaacaagt agcactctct cagaagatca gatgagccgt cttgccaagc
601tcaccaaagc tcatcgacaa ggacacatgg gtgaaagtag attggctgga tagattgaca
661tttagagaaa tagaaatgat aaatgagagt gaaaaacgaa gttctaattt catgtacctg
721atggttgaat ttcgatgtgt caagtgtgat gataaggaat atggtattgt ttattatgaa
781aaggacggtg atgaatcatc tccattttaa caagttttga attagtggaa agttcctgac
841ccccagatgt cttatggaga atttagttga gagcaaacac cacaagcttg cccggagttt
901aagaagtgga ccttctgacc acgatctgaa acccaatgct gccacgagag atcagttaaa
961tattattgtg agttatccac caaccaagca acttacatat gaagaacaag atcttgtttg
1021gaagtttaga tattatctta cgaatcaaga aaaagccttg acaaaattct tgaaatgtgt
1081taattgggat ctacctcaag aggccaaaca ggccttggaa cttctgggaa aatggaagcc
1141gatggatgta gaggactcct tggagctgtt atcctctcat tacaccaacc caactgtgag
1201gcgttatgct gttgcccggt tgcgacaggc cgatgatgag gatttgttga tgtacctatt
1261acaattggtc caggctctca aatatgaaaa ttttgatgat ataaagaatg gattggaacc
1321taccaagaag gatagtcaga gttcagtgtc agaaaatgtg tcaaattctg gaataaattc
1381tgcagaaata gatagctccc aaattataac cagccccctt ccttcagtct cttcacctcc
1441tcctgcatca aaaacaaaag aagttccaga tggcgaaaat ctggaacaag atctctgtac
1501cttcttgata tcgagagcct gcaaaaactc aacactggct aattatttat actggtatgt
1561gatagtggaa tgtgaagatc aagatactca gcagagagat ccaaagaccc atgagatgta
1621cttgaacgta atgagaagat tcagccaagc attgttgaag ggtgataagt ctgtcagagt
1681tatgcgttct ttgctggctg cacaacagac atttgtagat cggttggtgc atctaatgaa
1741ggcagtacaa cgcgaaagtg gaaatcgtaa gaaaaagaat gagagactac aggcattgct
1801tggagataat gaaaagatga atttgtcaga tgtggaactt atcccgttgc ctttagaacc
1861ccaagtgaaa attagaggaa taattccgga aacagctaca ctgtttaaaa gtgcccttat
1921gcctgcccag tgttttttaa gacgggagga tggaggaaaa tatcccagtt atatttaagc
1981atggagatga tttacgtcaa gatcaactta ttcttcaaat catttcactc atggacaagc
2041tgttacggaa agaaaatctg gactgaaaat tgacacctta taaggtgtta gccaccagta
2101caaaacatgg cttcatgcag tttatccagt cagttcctgt ggctgaagtt cttgatacag
2161agggaagcat tcagaacttt tttagaaaat atgcaccaag tgagaatggg ccaaatggga
2221ttagtgctga ggTCATGGAC ACTTACGTTA AAAGCTGTGC TGGATATTGC GTGATCACCT
2281ATATACTTGG AGTTGGAGAC AGGCACCTGG ATAACCTTTT GCTAACAAAA ACAGGCAAAC
2341TCTTCCACAT AGACTTTGGA TATATTTTGG GTCGGGATCC AAAGCCTCTT CCTCCACCAA
2401TGAAGCTGAA TAAAGAAATG GTAGAAGGAA TGGGGGGCAC ACAGAGTGAG CAGTACCAAG
2461AGTTCCGTAA ACAGTGTTAC ACGGCTTTCC TCCACCTGCG AAGGTATTCT AATCTGATTT
2521TGAACTTGTT TTCCTTGATG GTTGATGCAA ACATTCCAGA TATTGCACTT GAACCAGATA
2581AAACTGTGAA AAAGGTTCAG GATAAATTCC GCTTAGACCT GTCGGATGAA GAGGCTGTGC
2641ATTACATGCA GAGTCTGATT GATGAGAGTG TCCATGCTCT TTTTGCTGCA GTGGTGGAAC
2701AGATTCACAA GTTTGCCCAG TACTGGAGAA AATTGCCAAC TTTCTTGTAC AAAGTGGTTG
2761ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2821GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA
2881TCTTGGTAAC TGTATGAATA AAACGCGTTA AGTCgacaat caacctctgg attacaaaat
2941ttgtgaaaga tt

Download FASTA (ORF) (Full)