Construct: ORF TRCN0000475007
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018893.3_s317c1
- Derived from:
- ccsbBroadEn_14758
- DNA Barcode:
- AATCTTGGTAACTGTATGAATAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PIK3C3 (5289)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475007
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | NM_002647.4 | 99.5% | 21.3% | (many diffs) |
| 2 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | NM_001308020.2 | 92.4% | 14.2% | (many diffs) |
| 3 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XM_011526031.3 | 78.6% | 5.5% | (many diffs) |
| 4 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XR_002958176.1 | 41.4% | (many diffs) | |
| 5 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XR_935226.1 | 40% | (many diffs) | |
| 6 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XR_001753204.2 | 39% | (many diffs) | |
| 7 | mouse | 225326 | Pik3c3 | phosphatidylinositol 3-kina... | NM_181414.5 | 87.4% | 21% | (many diffs) |
| 8 | mouse | 225326 | Pik3c3 | phosphatidylinositol 3-kina... | XR_385985.2 | 58.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 651
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggaagca gagaagtttc actacatcta tagttgtgac ctggatatca 121 acgtccagct taagatagga agcttggaag ggaagagaga acaaaagagt tataaagctg 181 tcctggaaga cccaatgttg aagttctcag gactatatca agagacatgc tctgatcttt 241 atgttacttg tcaagttttt gcagaaggga agcctttggc cttgccagtg agaacatcct 301 acaaagcatt tagtacaaga tggaactgga atgaatggct gaaactacca gtaaaatacc 361 ctgacctgcc caggaatgcc caagtggccc tcaccatatg ggatgtgtat ggtcccggaa 421 aagcagtgcc tgtaggagga acaacggttt cgctctttgg aaaatacggc atgtttcgcc 481 aagggatgca tgacttgaaa gtctggccta atgtagaagc agatggatca gaacccacaa 541 aaactcctgg cagaacaagt agcactctct cagaagatca gatgagccgt cttgccaagc 601 tcaccaaagc tcatcgacaa ggacacatgg gtgaaagtag attggctgga tagattgaca 661 tttagagaaa tagaaatgat aaatgagagt gaaaaacgaa gttctaattt catgtacctg 721 atggttgaat ttcgatgtgt caagtgtgat gataaggaat atggtattgt ttattatgaa 781 aaggacggtg atgaatcatc tccattttaa caagttttga attagtggaa agttcctgac 841 ccccagatgt cttatggaga atttagttga gagcaaacac cacaagcttg cccggagttt 901 aagaagtgga ccttctgacc acgatctgaa acccaatgct gccacgagag atcagttaaa 961 tattattgtg agttatccac caaccaagca acttacatat gaagaacaag atcttgtttg 1021 gaagtttaga tattatctta cgaatcaaga aaaagccttg acaaaattct tgaaatgtgt 1081 taattgggat ctacctcaag aggccaaaca ggccttggaa cttctgggaa aatggaagcc 1141 gatggatgta gaggactcct tggagctgtt atcctctcat tacaccaacc caactgtgag 1201 gcgttatgct gttgcccggt tgcgacaggc cgatgatgag gatttgttga tgtacctatt 1261 acaattggtc caggctctca aatatgaaaa ttttgatgat ataaagaatg gattggaacc 1321 taccaagaag gatagtcaga gttcagtgtc agaaaatgtg tcaaattctg gaataaattc 1381 tgcagaaata gatagctccc aaattataac cagccccctt ccttcagtct cttcacctcc 1441 tcctgcatca aaaacaaaag aagttccaga tggcgaaaat ctggaacaag atctctgtac 1501 cttcttgata tcgagagcct gcaaaaactc aacactggct aattatttat actggtatgt 1561 gatagtggaa tgtgaagatc aagatactca gcagagagat ccaaagaccc atgagatgta 1621 cttgaacgta atgagaagat tcagccaagc attgttgaag ggtgataagt ctgtcagagt 1681 tatgcgttct ttgctggctg cacaacagac atttgtagat cggttggtgc atctaatgaa 1741 ggcagtacaa cgcgaaagtg gaaatcgtaa gaaaaagaat gagagactac aggcattgct 1801 tggagataat gaaaagatga atttgtcaga tgtggaactt atcccgttgc ctttagaacc 1861 ccaagtgaaa attagaggaa taattccgga aacagctaca ctgtttaaaa gtgcccttat 1921 gcctgcccag tgttttttaa gacgggagga tggaggaaaa tatcccagtt atatttaagc 1981 atggagatga tttacgtcaa gatcaactta ttcttcaaat catttcactc atggacaagc 2041 tgttacggaa agaaaatctg gactgaaaat tgacacctta taaggtgtta gccaccagta 2101 caaaacatgg cttcatgcag tttatccagt cagttcctgt ggctgaagtt cttgatacag 2161 agggaagcat tcagaacttt tttagaaaat atgcaccaag tgagaatggg ccaaatggga 2221 ttagtgctga ggTCATGGAC ACTTACGTTA AAAGCTGTGC TGGATATTGC GTGATCACCT 2281 ATATACTTGG AGTTGGAGAC AGGCACCTGG ATAACCTTTT GCTAACAAAA ACAGGCAAAC 2341 TCTTCCACAT AGACTTTGGA TATATTTTGG GTCGGGATCC AAAGCCTCTT CCTCCACCAA 2401 TGAAGCTGAA TAAAGAAATG GTAGAAGGAA TGGGGGGCAC ACAGAGTGAG CAGTACCAAG 2461 AGTTCCGTAA ACAGTGTTAC ACGGCTTTCC TCCACCTGCG AAGGTATTCT AATCTGATTT 2521 TGAACTTGTT TTCCTTGATG GTTGATGCAA ACATTCCAGA TATTGCACTT GAACCAGATA 2581 AAACTGTGAA AAAGGTTCAG GATAAATTCC GCTTAGACCT GTCGGATGAA GAGGCTGTGC 2641 ATTACATGCA GAGTCTGATT GATGAGAGTG TCCATGCTCT TTTTGCTGCA GTGGTGGAAC 2701 AGATTCACAA GTTTGCCCAG TACTGGAGAA AATTGCCAAC TTTCTTGTAC AAAGTGGTTG 2761 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2821 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 2881 TCTTGGTAAC TGTATGAATA AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2941 ttgtgaaaga tt