Construct: ORF TRCN0000475052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018986.1_s317c1
- Derived from:
- ccsbBroadEn_14788
- DNA Barcode:
- TTCAATCGGTGGGAGACCACTGAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKCA (5578)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475052
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5578 | PRKCA | protein kinase C alpha | NM_002737.3 | 99.5% | 33% | (many diffs) |
| 2 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024836.2 | 92.8% | 34.4% | (many diffs) |
| 3 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024837.1 | 90.8% | 23.1% | (many diffs) |
| 4 | human | 5578 | PRKCA | protein kinase C alpha | XM_024450829.1 | 85.9% | 19% | (many diffs) |
| 5 | human | 5578 | PRKCA | protein kinase C alpha | XM_024450830.1 | 85.9% | 19% | (many diffs) |
| 6 | human | 5578 | PRKCA | protein kinase C alpha | XM_017024841.1 | 46.8% | 68.5% | (many diffs) |
| 7 | mouse | 18750 | Prkca | protein kinase C, alpha | NM_011101.3 | 90.6% | 32.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 735
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgacgtt ttcccgggca acgactccac ggcgtctcag gacgtggcca 121 accgcttcgc ccgcaaaggg gcgctgaggc agaagaacgt gcacgaggtg aaggaccaca 181 aattcatcgc gcgcttcttc aagcagccca ccttctgcag ccactgcacc gacttcatct 241 gggggtttgg gaaacaaggc ttccagtgcc aagtttgctg ttttgtggtc cacaagaggt 301 gccatgaatt tgttactttt tcttgtccgg gtgcggataa gggacccgac actgatgacc 361 ccaggagcaa gcacaagttc aaaatccaca cttacggaag ccccaccttc tgcgatcact 421 gtgggtcact gctctatgga cttatccatc aagggatgaa atgtgacacc tgcgatatga 481 acgttcacaa gcaatgcgtc atcaatgtcc ccagcctctg cggaatggat cacactgaga 541 agagggggcg gatttaccta aaggctgagg ttgctgatga aaagctccat gtcacagtac 601 gagatgcaaa aaatctaatc cctatggatc caaacgggct ttcagatcct tatgtgaagc 661 tgaaacttat tcctgatccc aagaatgaaa gcaagcaaaa aaccaaaacc atccgctcca 721 cactaaatcc gcagtgaaat gaatccttta ctttcaattt gaaacctttc gacaaagacc 781 gacaactgtc tgtagaaatc tgggactggg aacgaacaac aaggaatgac ttcatgggat 841 ccctttcctt tggagtttcg gagctgatga agatgccggc cagtggatgg tacaagttgc 901 ttaaccaaga agaaggtgag tactacaacg tacccattcc ggaaggggac gaggaaggaa 961 acatggaact caggcagaaa ttcgagaaag ccaaacttgg ccctgctggc aacaaagtca 1021 tcagtccctc tgaagacagg aaacaacctt ccaacaacct tgaccgagtg aaactcacgg 1081 acttcaattt cctcatggtg ttgggaaagg ggagttttgg aaaggtgatg cttgccgaca 1141 ggaagggcac agaagaactg tatgcaatca aaatcctgaa gaaggatgtg gtgattcagg 1201 atgatgacgt ggagtgcacc atggtagaaa agcgagtctt ggccctgctt gacaaacccc 1261 cgttcttgac gcagctgcac tcctgcttcc agacagtgga tcggctgtac ttcgtcatgg 1321 aatatgtcaa cggtggggac ctcatgtacc acattcagca agtaggaaaa tttaaggaac 1381 cacaagcagt attctatgcg gcagagattt ccatcggatt gtctttcttc ataaaagagg 1441 aatcatttat agggatctga agttagataa cgtcatgttg gattcagaag gacatatcaa 1501 aattgctgac tttgggatgt gcaaggaaca catgatggat ggagtcacga ccaggacctt 1561 ctgtgggact ccagattata tcgccccaga gataatcgct tatcagccgt atggaaaatc 1621 tgtggactgg tgggcctatg gcgtcctgtt gtatgaaatg cttgccgggc agcctccatt 1681 tgatggtgaa gatgaagacg agctattTCA GTCTATCATG GAGCACAACG TTTCCTATCC 1741 AAAATCCTTG TCCAAGGAGG CTGTTTCTAT CTGCAAAGGA CTGATGACCA AACACCCAGC 1801 CAAGCGGCTG GGCTGTGGGC CTGAGGGGGA GAGGGACGTG AGAGAGCATG CCTTCTTCCG 1861 GAGGATCGAC TGGGAAAAAC TGGAGAACAG GGAGATCCAG CCACCATTCA AGCCCAAAGT 1921 GTGTGGCAAA GGAGCAGAGA ACTTTGACAA GTTCTTCACA CGAGGACAGC CCGTCTTAAC 1981 ACCACCTGAT CAGCTGGTTA TTGCTAACAT AGACCAGTCT GATTTTGAAG GGTTCTCGTA 2041 TGTCAACCCC CAGTTTGTGC ACCCCATCTT ACAGAGTGCA GTATTGCCAA CTTTCTTGTA 2101 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 2161 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 2221 AAAGGACGAT TCAATCGGTG GGAGACCACT GAGACGCGTT AAGTCgacaa tcaacctctg 2281 gattacaaaa tttgtgaaag att