Construct: ORF TRCN0000475064
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016642.1_s317c1
- Derived from:
- ccsbBroadEn_06145
- DNA Barcode:
- CCTGTTCTTTCTGAACACCACATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF1AX (1964)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475064
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1964 | EIF1AX | eukaryotic translation init... | NM_001412.4 | 99.7% | 99.3% | 10A>G |
| 2 | human | 9086 | EIF1AY | eukaryotic translation init... | NM_004681.4 | 91.8% | 98.6% | (many diffs) |
| 3 | human | 9086 | EIF1AY | eukaryotic translation init... | NM_001278612.1 | 81.4% | 86.8% | (many diffs) |
| 4 | mouse | 66235 | Eif1ax | eukaryotic translation init... | NM_025437.5 | 88.6% | 99.3% | (many diffs) |
| 5 | mouse | 13664 | Eif1a | eukaryotic translation init... | NM_010120.5 | 87.5% | 97.9% | (many diffs) |
| 6 | mouse | 100861898 | Gm21936 | predicted gene, 21936 | XM_006516436.2 | 86.1% | 90.9% | (many diffs) |
| 7 | mouse | 666806 | Gm8300 | predicted gene 8300 | NM_001177565.1 | 85.8% | 93% | (many diffs) |
| 8 | mouse | 100039052 | Gm2022 | predicted pseudogene 2022 | NM_001177574.1 | 85.6% | 92.3% | (many diffs) |
| 9 | mouse | 100861908 | Gm21319 | predicted gene, 21319 | NM_001270722.1 | 85.6% | 92.3% | (many diffs) |
| 10 | mouse | 100039042 | Gm2016 | predicted gene 2016 | NM_001122662.1 | 85.4% | 91.6% | (many diffs) |
| 11 | mouse | 435337 | Gm5662 | predicted gene 5662 | NM_001013824.3 | 85.1% | 91.6% | (many diffs) |
| 12 | mouse | 100042776 | Gm4027 | predicted gene 4027 | NM_001177564.1 | 84.9% | 89.5% | (many diffs) |
| 13 | mouse | 544881 | BB287469 | expressed sequence BB287469 | NM_001177573.1 | 84.9% | 89.5% | (many diffs) |
| 14 | mouse | 100042776 | Gm4027 | predicted gene 4027 | XM_011243969.1 | 84.9% | 89.5% | (many diffs) |
| 15 | mouse | 100503047 | LOC100503047 | eukaryotic translation init... | XM_003085585.1 | 83.5% | 86.2% | (many diffs) |
| 16 | mouse | 100039100 | Gm2046 | predicted gene 2046 | XM_017315327.1 | 74.3% | 78.4% | (many diffs) |
| 17 | mouse | 13664 | Eif1a | eukaryotic translation init... | XM_006525626.3 | 49.6% | 55.5% | (many diffs) |
| 18 | mouse | 266459 | Gm5039 | predicted gene 5039; eukary... | NR_003647.2 | 22.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 522
- ORF length:
- 432
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCCAAGGA TAAAGGTAAA GGAGGTAAAA 121 ACAGACGCAG GGGTAAGAAT GAGAATGAAT CTGAAAAAAG AGAACTGGTA TTCAAAGAGG 181 ATGGTCAGGA GTATGCTCAG GTAATCAAAA TGTTGGGAAA TGGACGGCTA GAAGCAATGT 241 GTTTCGATGG TGTAAAGAGG TTATGTCACA TCAGAGGAAA ATTGAGAAAA AAGGTTTGGA 301 TAAATACCTC GGACATTATT TTGGTTGGTC TCCGAGACTA CCAGGATAAC AAAGCTGATG 361 TAATTTTAAA ATACAATGCA GACGAAGCTA GAAGTCTGAA GGCATACGGC GAGCTTCCAG 421 AGCATGCTAA AATCAATGAA ACTGATACAT TTGGTCCTGG AGATGATGAT GAAATTCAGT 481 TTGATGACAT TGGAGATGAT GATGAAGATA TTGATGACAT CTACCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGACCT GTTCTTTCTG AACACCACAT CACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t