Construct: ORF TRCN0000475147
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015400.1_s317c1
- Derived from:
- ccsbBroadEn_10677
- DNA Barcode:
- TCATGCGTAATGATATACTCGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANGPT1 (284)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475147
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284 | ANGPT1 | angiopoietin 1 | NM_001314051.1 | 49.3% | 49.3% | 1_162del;205_207delGGT;607_894del |
| 2 | human | 284 | ANGPT1 | angiopoietin 1 | NM_001199859.3 | 29.5% | 29.5% | 1_762del;1204_1491del |
| 3 | human | 284 | ANGPT1 | angiopoietin 1 | NM_001146.5 | 29.5% | 29.5% | 1_762del;805_807delGGT;1207_1494del |
| 4 | human | 284 | ANGPT1 | angiopoietin 1 | XR_928319.2 | 10.4% | (many diffs) | |
| 5 | mouse | 11600 | Angpt1 | angiopoietin 1 | XM_006520323.2 | 34% | 36% | (many diffs) |
| 6 | mouse | 11600 | Angpt1 | angiopoietin 1 | NM_001286062.1 | 27.3% | 28.9% | (many diffs) |
| 7 | mouse | 11600 | Angpt1 | angiopoietin 1 | NM_009640.4 | 27.3% | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggCATGGA CACAGTCCAC AACCTTGTCA ATCTTTGCAC TAAAGAAGTT TTACTAAAGG 121 GAGGAAAAAG AGAGGAAGAG AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT 181 TTAATAAAAG TGGAATCTAC ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT 241 TTTGCAATAT GGATGTCAAT GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA 301 GTCTAGATTT CCAAAGAGGC TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG 361 AATATTGGCT GGGGAATGAG TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA 421 GAATTGAGTT AATGGACTGG GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA 481 TAGGAAATGA AAAGCAAAAC TATAGGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCATGCG 661 TAATGATATA CTCGCGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg 721 aaagatt