Construct: ORF TRCN0000475148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004446.1_s317c1
- Derived from:
- ccsbBroadEn_12850
- DNA Barcode:
- TGCTGTCGGCAGCCCTCCTCTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MGC10997 (84741)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475148
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 104472716 | WARS2-IT1 | WARS2 intronic transcript 1 | NR_126447.1 | 19.7% | (many diffs) | |
| 2 | human | 400121 | LINC00547 | long intergenic non-protein... | NR_040244.1 | 3.3% | (many diffs) | |
| 3 | human | 101928808 | LOC101928808 | uncharacterized LOC101928808 | XR_244519.1 | 2.8% | (many diffs) | |
| 4 | human | 101929048 | LOC101929048 | uncharacterized LOC101929048 | XR_001741387.1 | 2.3% | (many diffs) | |
| 5 | human | 283487 | LINC00346 | long intergenic non-protein... | NR_027701.1 | 1.8% | (many diffs) | |
| 6 | human | 162655 | ZNF519 | zinc finger protein 519 | NR_033354.2 | 1.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 446
- ORF length:
- 126
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGGGGGAAAG TTTGGAACTT CCTAGAGACT TGTTGAATGG 361 CTTTGACCAA AATGCTGATA ATGATGTGGT CAATGAAATC CAGGCTGAGG TGGTCTCAGA 421 TGGAGATGAG GAACTTGTTG GGAACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 481 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 541 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGCTGTCGG 601 CAGCCCTCCT CTTACACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 661 agatt