Construct: ORF TRCN0000475184
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007630.1_s317c1
- Derived from:
- ccsbBroadEn_12290
- DNA Barcode:
- GATTGAAATTTTCATGTTTAGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYO5C (55930)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475184
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55930 | MYO5C | myosin VC | XM_017022408.2 | 25.3% | 24.9% | 1005T>C;1273_1312del;1331_5091del |
| 2 | human | 55930 | MYO5C | myosin VC | XM_011521781.3 | 25% | 24.7% | 1005T>C;1273_1312del;1331_5142del |
| 3 | human | 55930 | MYO5C | myosin VC | NM_018728.4 | 24.6% | 24.3% | 1005T>C;1273_1312del;1331_5226del |
| 4 | mouse | 208943 | Myo5c | myosin VC | XR_001778836.1 | 30.7% | (many diffs) | |
| 5 | mouse | 208943 | Myo5c | myosin VC | XM_006510940.3 | 30% | 32.4% | (many diffs) |
| 6 | mouse | 208943 | Myo5c | myosin VC | XM_006510939.2 | 28.8% | 31% | (many diffs) |
| 7 | mouse | 208943 | Myo5c | myosin VC | NM_001081322.1 | 21.4% | 23.1% | (many diffs) |
| 8 | mouse | 208943 | Myo5c | myosin VC | XM_006510935.3 | 21.3% | 23% | (many diffs) |
| 9 | mouse | 208943 | Myo5c | myosin VC | XM_006510936.3 | 21.3% | 23% | (many diffs) |
| 10 | mouse | 208943 | Myo5c | myosin VC | XM_006510937.3 | 21.3% | 23% | (many diffs) |
| 11 | mouse | 208943 | Myo5c | myosin VC | XM_011242693.2 | 21.3% | 23% | (many diffs) |
| 12 | mouse | 208943 | Myo5c | myosin VC | XR_001778835.1 | 20.6% | (many diffs) | |
| 13 | mouse | 208943 | Myo5c | myosin VC | XR_379407.3 | 16.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1356
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtggccgag ctgtacacgc agtacaacag ggtctggatt cccgatcctg 121 aagaagtttg gaagtctgct gaaatagcca aggactacag agttggtgac aaggtcctgc 181 gactcctgct ggaggatgga acggagctgg attattctgt caatccagaa tctctgcctc 241 cacttcggaa tcctgacatc ctcgtgggcg agaatgacct cacggctctc agctatcttc 301 acgagcccgc ggtgctccac aacctcagaa tccgctttgc agaatccaaa ctcatttaca 361 cctacagtgg aatcattttg gtggccatga atccttacaa gcagttgcca atatacggag 421 atgccatcat ccacgcctac agcgggcaga acatgggcga tatggaccca cacatatttg 481 ccgtggcaga agaggcatac aagcagatgg ccagaaacaa cagaaaccag tccataattg 541 taagtgggga gtcaggtgct ggaaagacag tgtcggctcg ctatgccatg aggtactttg 601 ccaccgtcag caaatcgggc agcaacgctc acgtggaaga caaggtcctg gcatccaatc 661 ccatcaccga ggccgttgga aatgccaaga ccacccgcaa tgacaatagt agtcggtttg 721 ggaaatacac agaaatcagt tttgatgaac aaaatcaaat tataggagcc aacatgagca 781 cttacctcct ggagaaatcc agagttgtct ttcaatcgga aaatgaacga aattaccaca 841 ttttctatca gctttgtgca tctgcacagc agtcggaatt taaacatctt aaattgggga 901 gtgccgaaga atttaattat acaagaatgg gaggcaatac tgTCATTGAG GGTGTGAATG 961 ATCGAGCTGA AATGGTAGAG ACTCAAAAGA CCTTCACGCT TCTGGGTTTC AAGGAGGATT 1021 TTCAGATGGA CGTTTTTAAA ATCCTGGCAG CCATCCTACA TCTGGGCAAC GTGCAGATCA 1081 CCGCGGTGGG CAACGAGAGG TCCTCAGTTA GTGAGGATGA CAGTCACCTG AAGGTGTTCT 1141 GTGAGCTCCT GGGCCTGGAG AGTGGCAGAG TTGCTCAGTG GCTGTGCAAT CGCAAAATCG 1201 TCACAAGCTC TGAGACGGTG GTAAAACCCA TGACCAGGCC TCAGGCTGTC AACGCCAGGG 1261 ATGCACTGGC CAAAAAGATC TATGCTCACC TGTTCGACTT CATTGTGGAG AGAATTAACC 1321 AAGCGTTGCA GTTTTCAGTT TTGAAACCTT TGATGTGCCC AACTTTCTTG TACAAAGTGG 1381 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1441 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATTTGTG GAAAGGACGA 1501 GATTGAAATT TTCATGTTTA GAGTACGCGT TAAGTCgaca atcaacctct ggattacaaa 1561 atttgtgaaa gatt