Construct: ORF TRCN0000475199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003496.3_s317c1
- Derived from:
- ccsbBroadEn_15160
- DNA Barcode:
- TTCCACCGTCTCTGGCTTCGCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PANK2 (80025)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475199
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_001324193.2 | 100% | 100% | |
| 2 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_017028077.2 | 100% | 100% | |
| 3 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_017028078.2 | 100% | 100% | |
| 4 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_017028079.2 | 100% | 100% | |
| 5 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_024452002.1 | 100% | 100% | |
| 6 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_001324191.2 | 48% | 48% | 1_435del |
| 7 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_024960.6 | 48% | 48% | 1_435del |
| 8 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_153640.3 | 48% | 48% | 1_435del |
| 9 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_005260836.4 | 48% | 48% | 1_435del |
| 10 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_005260835.3 | 36.7% | 36.7% | 1_693del |
| 11 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_011529364.3 | 24.9% | 21.2% | (many diffs) |
| 12 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_153638.3 | 23.5% | 23.5% | 1_1308del |
| 13 | human | 80025 | PANK2 | pantothenate kinase 2 | NR_136715.2 | 5.1% | 1_879del;1282_7877del | |
| 14 | human | 80025 | PANK2 | pantothenate kinase 2 | XR_002958533.1 | 3.2% | 1_2096del;2499_12294del | |
| 15 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_017319300.1 | 90.3% | 98.5% | (many diffs) |
| 16 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_017319299.1 | 61.7% | 67.3% | (many diffs) |
| 17 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_006500298.3 | 43.4% | 47.3% | (many diffs) |
| 18 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_006500299.3 | 43.4% | 47.3% | (many diffs) |
| 19 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_017319298.1 | 43.4% | 47.3% | (many diffs) |
| 20 | mouse | 74450 | Pank2 | pantothenate kinase 2 | NM_153501.2 | 27.3% | 29.7% | (many diffs) |
| 21 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XR_001783191.1 | 8.2% | (many diffs) | |
| 22 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XR_001783192.1 | 5.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC ATCTCGTGGA GATAGCACCA AAGTGGATAA ACTAGTACGA GATATTTATG 121 GAGGGGACTA TGAGAGGTTT GGACTGCCAG GCTGGGCTGT GGCTTCAAGC TTTGGAAACA 181 TGATGAGCAA GGAGAAGCGA GAGGCTGTCA GTAAAGAGGA CCTGGCCAGA GCGACTTTGA 241 TCACCATCAC CAACAACATT GGCTCAATAG CAAGAATGTG TGCCCTTAAT GAAAACATTA 301 ACCAGGTGGT ATTTGTTGGA AATTTCTTGA GAATTAATAC GATCGCCATG CGGCTTTTGG 361 CATATGCTTT GGATTATTGG TCCAAGGGGC AGTTGAAAGC ACTTTTTTCG GAACACGAGG 421 GTTATTTTGG AGCTGTTGGA GCACTCCTTG AGCTGTTGAA GATCCCGTGC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGATTCCACC GTCTCTGGCT TCGCAATACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt