Construct: ORF TRCN0000475203

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002821.1_s317c1
Derived from:
ccsbBroadEn_14466
DNA Barcode:
CGTCGGAGACTTGGCAAAATACGA
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CEP57L1 (285753)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475203
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350665.1 99.8% 98.4% 741delA
2 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350666.1 93.4% 92% 338_339ins48;693delA
3 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_011535763.2 81.7% 81.3% (many diffs)
4 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001271853.2 63.7% 63.1% (many diffs)
5 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_024446405.1 63.7% 63.1% (many diffs)
6 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_024446406.1 59.6% 59% (many diffs)
7 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001083535.2 54.1% 53.6% (many diffs)
8 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001271852.2 54.1% 53.6% (many diffs)
9 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350652.1 54.1% 53.6% (many diffs)
10 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350653.1 54.1% 53.6% (many diffs)
11 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_173830.5 54.1% 53.6% (many diffs)
12 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_017010769.1 53.8% 53.3% (many diffs)
13 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350654.1 52.2% 51.7% (many diffs)
14 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350655.1 52.2% 51.7% (many diffs)
15 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350656.1 52.2% 51.7% (many diffs)
16 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350657.1 52.2% 51.7% (many diffs)
17 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350658.1 52.2% 51.7% (many diffs)
18 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_006715462.3 52.2% 51.7% (many diffs)
19 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_017010768.2 52.2% 51.7% (many diffs)
20 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_024446404.1 51.9% 51.4% (many diffs)
21 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_005266938.4 50% 49.5% (many diffs)
22 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350659.1 48.9% 48.4% (many diffs)
23 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_005266935.5 48.4% 47.9% (many diffs)
24 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_005266936.4 48.4% 47.9% (many diffs)
25 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_005266937.4 45.3% 44.8% (many diffs)
26 human 285753 CEP57L1 centrosomal protein 57 like 1 NR_146889.1 28.6% (many diffs)
27 human 285753 CEP57L1 centrosomal protein 57 like 1 NR_146888.1 25.4% (many diffs)
28 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350662.1 24.2% 23.6% (many diffs)
29 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350663.1 24.2% 23.6% (many diffs)
30 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350664.1 24.2% 23.6% (many diffs)
31 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350660.1 23.3% 22.8% (many diffs)
32 human 285753 CEP57L1 centrosomal protein 57 like 1 NM_001350661.1 23.3% 22.8% (many diffs)
33 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_011535762.2 20.5% 19.4% (many diffs)
34 human 285753 CEP57L1 centrosomal protein 57 like 1 XM_017010771.1 20.5% 19.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
819
ORF length:
753
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ttctgaatta atgcatagta tagtaggaag ctatcataaa cctccagaaa
121gagtatttgt tccctcattc acccagaatg aaccatctca gaattgccat cctgcaaact
181tagaagttac ctctcctaag atacttcata gcccaaatag ccaagctctt attttagcct
241taaaaactct tcaggaaaaa attcatcgtt tagagctgga gagaacacaa gctgaagata
301acctgaacat tctttccaga gaagcagcac agtataagaa ggccTTAGAG AATGAAACAA
361ATGAGAGAAA TCTGGCACAC CAGGAGCTGA TAAAGCAGAA AAAAGATATA AGTATACAGT
421TAAGCTCAGC CCAGTCTCGT TGTACTCTTC TAGAGAAGCA ACTAGAATAT ACAAAGAGAA
481TGGTTCTCAA CGTAGAGCGA GAAAAGAACA TGATCCTAGA ACAACAGGCC CAGCTTCAGA
541GGGAAAAAGA ACAAGATCAG ATGAAGCTGT ATGCAAAACT TGAAAAGCTT GATGTCTTAG
601AAAAAGAGTG TTTCAGACTT ACAACAACTC AGAAAACTGC TGAGGACAAG ATTAAACATT
661TAGAAGAAAA ACTTAAGGAA GAAGAACATC AGCGTAAGCT ATTTCAAGAC AAAGCTTCTG
721AGCTTCAAAC TGGACTTGAA ATCAGTAAAA TTATAATGTC TTCAGTTTCA AATCTAAAGC
781ACTCCAAGGA AAAGAAGAAA TCTTCAAGGT GTGCATATAC CCAACTTTCT TGTACAAAGT
841GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG
901AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA
961CGACGTCGGA GACTTGGCAA AATACGAACG CGTTAAGTCg acaatcaacc tctggattac
1021aaaatttgtg aaagatt

Download FASTA (ORF) (Full)