Construct: ORF TRCN0000475259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016190.1_s317c1
- Derived from:
- ccsbBroadEn_02135
- DNA Barcode:
- GCCCTCTTCTGATACGAGTCATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTER (9317)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475259
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9317 | PTER | phosphotriesterase related | NM_001001484.2 | 100% | 100% | |
| 2 | human | 9317 | PTER | phosphotriesterase related | NM_001261836.1 | 100% | 100% | |
| 3 | human | 9317 | PTER | phosphotriesterase related | NM_030664.4 | 100% | 100% | |
| 4 | human | 9317 | PTER | phosphotriesterase related | XM_017016927.2 | 94.3% | 94.3% | 1_63del |
| 5 | human | 9317 | PTER | phosphotriesterase related | NM_001261837.1 | 86.5% | 86.5% | 696_697ins141 |
| 6 | human | 9317 | PTER | phosphotriesterase related | XM_017016929.2 | 86.5% | 86.5% | 696_697ins141 |
| 7 | human | 9317 | PTER | phosphotriesterase related | XM_017016930.2 | 86.5% | 86.5% | 696_697ins141 |
| 8 | human | 9317 | PTER | phosphotriesterase related | XM_017016928.2 | 77.2% | 77.2% | 1_126del;822_823ins141 |
| 9 | human | 9317 | PTER | phosphotriesterase related | NM_001261838.1 | 57.3% | 57.3% | 0_1ins447 |
| 10 | human | 9317 | PTER | phosphotriesterase related | XR_001747258.1 | 31.6% | 1_48del;1096_3311del | |
| 11 | mouse | 19212 | Pter | phosphotriesterase related | NM_008961.3 | 84.7% | 87.6% | (many diffs) |
| 12 | mouse | 19212 | Pter | phosphotriesterase related | NM_001305437.1 | 81.9% | 84.7% | (many diffs) |
| 13 | mouse | 19212 | Pter | phosphotriesterase related | XM_017316438.1 | 81.9% | 84.7% | (many diffs) |
| 14 | mouse | 19212 | Pter | phosphotriesterase related | XM_006497402.1 | 69% | 71.6% | (many diffs) |
| 15 | mouse | 19212 | Pter | phosphotriesterase related | XM_006497401.1 | 66.7% | 69.2% | (many diffs) |
| 16 | mouse | 19212 | Pter | phosphotriesterase related | NM_001305436.1 | 63.7% | 64.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttccttaagt ggaaaagtcc aaaccgtttt gggccttgta gagccaagca 121 aactgggccg taccctgacc catgaacacc tggccatgac ctttgactgc tgttactgtc 181 cacctccccc gtgccaggaa gctatttcca aagaacctat cgtgatgaaa aatttatatt 241 ggattcagaa aaacgcctat tcccataaag aaaaccttca attaaatcag gagacagaag 301 ccataaagga agaactgttg tattttaaag ctaatggtgg aggggctttg gtggaaaaca 361 caaccactgg gattagccga gacacacaga cgttgaagag gcttgcagaa gagactggcg 421 tccatatcat atctggagcc gggttttatg tggatgcaac tcactcctca gagaccaggg 481 ccatgtcagt ggagcagctt accgatgtcc ttatgaatga aattctccat ggagctgatg 541 gaaccagtat caagtgtggc attattggag aaattggttg ctcctggcct ttgactgaga 601 gtgaaagaaa ggttctccag gccacagctc atgcccaggc tcagcttggt tgtcctgtta 661 ttatccatcc tggacggagc tccagggcac catttcagat tatccgaata ttgcaagaag 721 caggcgcaga catctccaaa acagtcatgt cacacctGGA TAGGACTATT CTTGATAAGA 781 AAGAGCTCTT GGAGTTTGCT CAACTTGGCT GCTACTTGGA ATATGATCTC TTTGGTACTG 841 AACTACTTCA TTACCAACTC GGCCCAGATA TTGACATGCC TGATGATAAC AAAAGAATTA 901 GAAGGGTGCG TCTCCTGGTG GAAGAGGGCT GTGAAGATCG AATTCTGGTA GCACATGACA 961 TACATACGAA AACCCGGCTG ATGAAATATG GAGGTCACGG CTATTCTCAT ATACTCACCA 1021 ATGTTGTTCC TAAAATGTTG CTGAGAGGCA TAACTGAGAA TGTGCTTGAT AAGATTCTAA 1081 TAGAGAACCC TAAGCAATGG CTAACTTTCA AATACCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 1261 CCTCTTCTGA TACGAGTCAT CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt