Construct: ORF TRCN0000475311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007393.1_s317c1
- Derived from:
- ccsbBroadEn_08572
- DNA Barcode:
- ACGTCTAAACTGCGCTGTATTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HHAT (55733)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475311
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55733 | HHAT | hedgehog acyltransferase | NM_001122834.3 | 99.8% | 99.7% | 534G>C;545G>A |
| 2 | human | 55733 | HHAT | hedgehog acyltransferase | NM_001170580.3 | 99.8% | 99.7% | 534G>C;545G>A |
| 3 | human | 55733 | HHAT | hedgehog acyltransferase | NM_018194.6 | 99.8% | 99.7% | 534G>C;545G>A |
| 4 | human | 55733 | HHAT | hedgehog acyltransferase | NM_001170587.2 | 93.9% | 90.6% | (many diffs) |
| 5 | human | 55733 | HHAT | hedgehog acyltransferase | XM_006711441.3 | 93.4% | 93.3% | 1_102del;636G>C;647G>A |
| 6 | human | 55733 | HHAT | hedgehog acyltransferase | XM_011509739.2 | 92% | 88% | (many diffs) |
| 7 | human | 55733 | HHAT | hedgehog acyltransferase | XM_011509740.2 | 92% | 88% | (many diffs) |
| 8 | human | 55733 | HHAT | hedgehog acyltransferase | XM_011509741.2 | 92% | 88% | (many diffs) |
| 9 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001729.1 | 92% | 88% | (many diffs) |
| 10 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001734.1 | 90.7% | 89.2% | (many diffs) |
| 11 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001728.1 | 87.7% | 83.9% | (many diffs) |
| 12 | human | 55733 | HHAT | hedgehog acyltransferase | XM_011509747.1 | 87% | 87% | 0_1ins189;345G>C;356G>A |
| 13 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001736.1 | 87% | 87% | 0_1ins189;345G>C;356G>A |
| 14 | human | 55733 | HHAT | hedgehog acyltransferase | NM_001170588.2 | 86.6% | 86.6% | 270_271ins195;339G>C;350G>A |
| 15 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001732.1 | 86.6% | 86.6% | 270_271ins195;339G>C;350G>A |
| 16 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001735.1 | 86.6% | 86.6% | 270_271ins195;339G>C;350G>A |
| 17 | human | 55733 | HHAT | hedgehog acyltransferase | XM_011509738.2 | 86.6% | 79.9% | (many diffs) |
| 18 | human | 55733 | HHAT | hedgehog acyltransferase | XM_011509737.2 | 86.4% | 82.7% | (many diffs) |
| 19 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001730.1 | 83.4% | 78.2% | (many diffs) |
| 20 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001731.1 | 83.4% | 78.2% | (many diffs) |
| 21 | human | 55733 | HHAT | hedgehog acyltransferase | XM_011509744.2 | 82.9% | 78.4% | (many diffs) |
| 22 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001733.1 | 80% | 76.1% | (many diffs) |
| 23 | human | 55733 | HHAT | hedgehog acyltransferase | XM_011509746.2 | 79.6% | 75.7% | (many diffs) |
| 24 | human | 55733 | HHAT | hedgehog acyltransferase | XM_011509742.2 | 74.8% | 71.1% | (many diffs) |
| 25 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001726.1 | 73.9% | 68.4% | (many diffs) |
| 26 | human | 55733 | HHAT | hedgehog acyltransferase | NM_001170564.2 | 72.2% | 72.2% | 271_272ins411 |
| 27 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001737.1 | 72.2% | 72.2% | 271_272ins411 |
| 28 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001738.1 | 66% | 62.3% | (many diffs) |
| 29 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001739.1 | 56.4% | 36.4% | (many diffs) |
| 30 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001740.1 | 56.4% | 36.4% | (many diffs) |
| 31 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001741.1 | 56.4% | 36.4% | (many diffs) |
| 32 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001742.1 | 56.4% | 36.4% | (many diffs) |
| 33 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001743.1 | 56.4% | 36.4% | (many diffs) |
| 34 | human | 55733 | HHAT | hedgehog acyltransferase | XM_017001744.1 | 56.4% | 36.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1548
- ORF length:
- 1479
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgccccga tgggaactgg cactttacct acttgcctca ctaggcttcc 121 acttctattc cttctatgaa gtttacaaag tctccagaga acacgaagag gagctggacc 181 aggaatttga gctggagact gacactttat ttggaggatt aaagaaggat gcgaccgact 241 ttgagtggag cttctggatg gaatggggga agcagtggct ggtgtggctt ctccttggcc 301 acatggtagt gtctcaaatg gccacactgc tggcaagaaa gcacagaccc tggattctca 361 tgctctatgg gatgtgggcc tgctggtgtg tgctggggac ccctggtgtg gctatggttt 421 tgctccatac caccatctct ttctgcgtgg cccagttccg gtctcagctc ctgacgtggc 481 tctgttctct cctcctcctc tccacactga ggctgcaggg tgtggaagaa gttaagagaa 541 ggtggtacaa gacagaaaac gagtactacc tgctgcagtt cacgctgacc gttcgctgcc 601 tctactacac caacttcagc ctggagctct gctggcagca gctgcctgct gcatcgacct 661 cctactcctt tccctggatg ctggcctatg tcttttatta tccagtctta cacaatgggc 721 ccatcctcag cttctcggag ttcatcaaac agatgcagca gcaggagcat gactccctga 781 aggccagcct gtgtgtcctg gccctggggc tgggccgcct tctttgctgg tggtggctgg 841 ccgagctgat ggctcacctg atgtacatgc atgccatcta cagcagcatc cccctcctgg 901 agactgtctc ttgttggacc ttaggaggac tggcgttagc ccaggtgctc tttttctacg 961 tgaagtactt ggtgctcttt ggcgtgcctg ctctgctcat gcgcctggat ggactcactc 1021 cacccgccct cccccgctgc gtgagcacca tgttcagttt caccgggatg tggaggtatt 1081 ttgatgttgg actgcataat ttcttaatca ggtatgtgta cattccagtg ggcgggtccc 1141 agcatggcct gctggggaca ctgttttcca cggcgatgac atttgcattt gtgagctact 1201 ggcatggcgg ctacgactac ctcTGGTGCT GGGCAGCGCT CAACTGGCTG GGAGTCACTG 1261 TGGAGAATGG AGTCCGGAGG CTGGTGGAGA CTCCCTGCAT CCAGGACAGT CTGGCCCGAT 1321 ACTTCTCCCC ACAAGCTCGC CGTCGATTCC ACGCTGCCCT TGCTTCTTGT TCCACCTCGA 1381 TGCTGATCCT GTCCAACCTG GTATTTCTTG GGGGCAATGA GGTTGGGAAA ACCTACTGGA 1441 ATAGGATCTT CATACAAGGC TGGCCTTGGG TGACCCTCTC TGTCCTGGGA TTCCTGTACT 1501 GCTACTCCCA CGTGGGCATT GCCTGGGCCC AGACCTACGC CACGGACTTG CCAACTTTCT 1561 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1621 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1681 GTGGAAAGGA CGAACGTCTA AACTGCGCTG TATTCCTACG CGTTAAGTCg acaatcaacc 1741 tctggattac aaaatttgtg aaagatt