Construct: ORF TRCN0000475371
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009972.1_s317c1
- Derived from:
- ccsbBroadEn_01873
- DNA Barcode:
- GCTAAGTTTATGTTCCCTATCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYN3 (8224)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475371
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8224 | SYN3 | synapsin III | NM_001135774.2 | 100% | 100% | |
| 2 | human | 8224 | SYN3 | synapsin III | NM_001369909.1 | 100% | 100% | |
| 3 | human | 8224 | SYN3 | synapsin III | NM_001369910.1 | 100% | 100% | |
| 4 | human | 8224 | SYN3 | synapsin III | NM_001369907.1 | 99.8% | 99.8% | 460_462delAGC |
| 5 | human | 8224 | SYN3 | synapsin III | NM_001369908.1 | 99.8% | 99.8% | 460_462delAGC |
| 6 | human | 8224 | SYN3 | synapsin III | NM_003490.4 | 99.8% | 99.8% | 460_462delAGC |
| 7 | human | 8224 | SYN3 | synapsin III | XM_011530405.3 | 99.8% | 99.8% | 460_462delAGC |
| 8 | human | 8224 | SYN3 | synapsin III | XM_017028961.2 | 99.8% | 99.8% | 460_462delAGC |
| 9 | human | 8224 | SYN3 | synapsin III | XM_017028962.2 | 99.8% | 99.8% | 460_462delAGC |
| 10 | human | 8224 | SYN3 | synapsin III | XM_017028963.2 | 99.8% | 99.8% | 460_462delAGC |
| 11 | human | 8224 | SYN3 | synapsin III | XM_011530410.3 | 79.2% | 78.7% | (many diffs) |
| 12 | human | 8224 | SYN3 | synapsin III | NM_133633.3 | 76.2% | 75.8% | (many diffs) |
| 13 | human | 8224 | SYN3 | synapsin III | XM_017028965.2 | 52.6% | 52.6% | 0_1ins822 |
| 14 | human | 8224 | SYN3 | synapsin III | XR_001755317.2 | 52.4% | (many diffs) | |
| 15 | mouse | 27204 | Syn3 | synapsin III | NM_013722.3 | 87.7% | 92.4% | (many diffs) |
| 16 | mouse | 27204 | Syn3 | synapsin III | XM_006513698.2 | 87.7% | 92.4% | (many diffs) |
| 17 | mouse | 27204 | Syn3 | synapsin III | XM_006513699.3 | 87.7% | 92.4% | (many diffs) |
| 18 | mouse | 27204 | Syn3 | synapsin III | XM_006513700.3 | 87.7% | 92.4% | (many diffs) |
| 19 | mouse | 27204 | Syn3 | synapsin III | XM_011243459.2 | 50.1% | 49.5% | (many diffs) |
| 20 | mouse | 27204 | Syn3 | synapsin III | XM_011243460.2 | 49.3% | 51.6% | (many diffs) |
| 21 | mouse | 27204 | Syn3 | synapsin III | XM_006513702.3 | 41.5% | 36.9% | (many diffs) |
| 22 | mouse | 27204 | Syn3 | synapsin III | XM_011243461.2 | 40.6% | 41.6% | (many diffs) |
| 23 | mouse | 27204 | Syn3 | synapsin III | NM_001164495.1 | 36.8% | 38.5% | (many diffs) |
| 24 | mouse | 27204 | Syn3 | synapsin III | XM_011243462.2 | 36.5% | 38.1% | (many diffs) |
| 25 | mouse | 27204 | Syn3 | synapsin III | XM_006513704.3 | 36.2% | 38.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1806
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatttcctc cggcgacgtc tctctgacag cagcttcatg gccaacctgc 121 ctaatggcta tatgacggac ctgcaacgcc cagatagctc caccagctca cctgcttccc 181 ccgccatgga gaggaggcac ccccagcccc tggctgcctc cttctcctct ccaggatcca 241 gcctttttag ctccctctcc agtgccatga agcaggcccc tcaggccacc tcaggactga 301 tggagcctcc aggtccctcc acgcccattg ttcaaagacc caggatcctg ttggtgatcg 361 atgatgccca tacagactgg tcgaagtatt tccatgggaa gaaggtgaat ggagagattg 421 agatccgagt ggagcaggct gaattctcag agttgaacct agctgcctat gtgaccgggg 481 gctgcatggt ggacatgcag gtcgtgagaa atgggaccaa agtggtgaga tccttcaagc 541 cagacttcat cctggtccgc cagcatgcct acagcatggc cctgggggaa gactaccgca 601 gcctggtcat cggcctgcag tatggagggc tgcctgctgt caactctctc tactccgtct 661 acaacttctg cagcaagccc tgggtgttct ctcagctcat taagatcttc cattccctgg 721 gtcctgagaa gttcccgctt gtggagcaaa catttttccc caaccataag ccaatggtca 781 cagccccaca cttcccggtg gtagtcaagc tgggacatgc ccacgctgga atgggaaaga 841 tcaaagtgga aaaccagctt gacttccagg acatcaccag cgtggtcgcc atggccaaaa 901 cctacgccac caccgaggcc ttcatcgact ccaagtacga catccgcatc cagaaaattg 961 gatccaacta caaggcttac atgagaacct ccatctctgg gaactggaag gccaacacag 1021 gctctgccat gctggagcag gtggccatga cagagaggta caggctgtgg gtggacagct 1081 gctcggaaat gtttggcggc ctggacatct gtgccgtcaa ggctgtccac agcaaggatg 1141 gcagagatta catcatcgag gtaatggaca gctcaatgcc gctgattgga gagcatgtgg 1201 aagaggacag acagctgatg gccgaccttg ttgtctccaa aatgagccag ctcccgatgc 1261 caggaggcac agcgccctcc cccctcagac cttgggctcc acagattaaa tcagcgaaat 1321 ccccagggca agcccagctg gggcctcagc taggccagcc ccagccacgc ccacctccgc 1381 aaggaggccc tcgccaagct cagtctcctc agccccagag atctggaagc ccctcccaac 1441 agaggctctc cccacaaggc cagcagcccc TGAGCCCCCA GTCCGGATCT CCACAGCAGC 1501 AAAGGTCACC AGGCTCTCCG CAGCTATCCC GGGCATCCAG TGGCAGCTCC CCAAACCAGG 1561 CCTCCAAGCC AGGTGCCACC CTCGCCTCAC AGCCCCGGCC CCCTGTGCAG GGCCGTAGTA 1621 CCTCCCAGCA GGGTGAAGAG TCCAAGAAGC CAGCACCACC CCATCCGCAT CTCAACAAAT 1681 CTCAGTCCCT GACTAACAGC CTCAGCACAT CCGACACCTC CCAGCGTGGG ACCCCAAGTG 1741 AAGACGAGGC CAAGGCTGAA ACCATCCGCA ACCTGAGGAA GTCTTTTGCC AGCCTGTTCT 1801 CTGACTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1861 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1921 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCTAAGTTT ATGTTCCCTA TCCCTACGCG 1981 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt