Construct: ORF TRCN0000475388
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002420.1_s317c1
- Derived from:
- ccsbBroadEn_13807
- DNA Barcode:
- CAGGCCGACAGAGACACGAAATCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ACTA1 (58)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475388
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58 | ACTA1 | actin alpha 1, skeletal muscle | NM_001100.4 | 99.9% | 1.8% | 4delT |
| 2 | human | 60 | ACTB | actin beta | NM_001101.5 | 85.8% | 1.8% | (many diffs) |
| 3 | human | 71 | ACTG1 | actin gamma 1 | NM_001199954.2 | 85.8% | 1.8% | (many diffs) |
| 4 | human | 71 | ACTG1 | actin gamma 1 | NM_001614.5 | 85.8% | 1.8% | (many diffs) |
| 5 | human | 70 | ACTC1 | actin alpha cardiac muscle 1 | NM_005159.5 | 85.5% | 1.8% | (many diffs) |
| 6 | human | 71 | ACTG1 | actin gamma 1 | NR_037688.3 | 54.6% | (many diffs) | |
| 7 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_001272041.1 | 90.8% | 1.8% | (many diffs) |
| 8 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_009606.3 | 90.8% | 1.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 120
- ORF length:
- 54
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tctgcatggc gacgaagacg agaccaccgc cctcgtgtgc gacaatggct ccggcctggt 121 gaaagccggc ttcgccgggg atgacgcccc tagggccgtg ttcccgtcca tcgtgggccg 181 cccccgacac cagggcgtca tggtcggtat gggtcagaaa gattcctacg tgggcgacga 241 ggctcagagc aagagaggta tcctgaccct gaagtaccct atcgagcacg gcatcatcac 301 caactgggat gacatggaga agatctggca ccacaccttc tacaacgagc ttcgcgtggc 361 tcccgaggag caccccaccc tgctcaccga ggcccccctc aatcccaagg ccaaccgcga 421 gaagatgacc cagatcatgt ttgagacctt caacgtgccc gccatgtacg tggccatcca 481 ggccgtgctg tccctctacg cctccggcag gaccaccggc atcgtgctgg actccggcga 541 cggcgtcacc cacaacgtgc ccatttatga gggctacgcg ctgccgcacg ccatcatgcg 601 cctggacctg gcgggccgcg atctcaccga ctacctgatg aagatcctca ctgagcgtgg 661 ctactccttc gtgaccacag ctgagcgcga gatcgtgcgc gacatcaagg agaagctgtg 721 ctacgtggcc ctggacttcg agaacgagat ggcgacggcc gcctcctcct cctccctgga 781 aaagagctac gagctgccag acgggcaggt catcaccatc ggcaacgagc gcttccgctg 841 cccggagacg ctcttccagc ccTCCTTCAT CGGTATGGAG TCGGCGGGCA TTCACGAGAC 901 CACCTACAAC AGCATCATGA AGTGTGACAT CGACATCAGG AAGGACCTGT ATGCCAACAA 961 CGTCATGTCG GGGGGCACCA CGATGTACCC TGGGATCGCT GACCGCATGC AGAAAGAGAT 1021 CACCGCGCTG GCACCCAGCA CCATGAAGAT CAAGATCATC GCCCCGCCGG AGCGCAAATA 1081 CTCGGTGTGG ATCGGCGGCT CCATCCTGGC CTCGCTGTCC ACCTTCCAGC AGATGTGGAT 1141 CACCAAGCAG GAGTACGACG AGGCCGGCCC TTCCATCGTC CACCGCAAAT GCTTCTACCC 1201 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 1261 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 1321 TATATCTTGT GGAAAGGACG ACAGGCCGAC AGAGACACGA AATCCACGCG TTAAGTCgac 1381 aatcaacctc tggattacaa aatttgtgaa agatt