Construct: ORF TRCN0000475406
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018857.3_s317c1
- Derived from:
- ccsbBroadEn_14921
- DNA Barcode:
- TTCCAGGTCAAGTCGTGACATCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDKL2 (8999)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475406
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8999 | CDKL2 | cyclin dependent kinase like 2 | XM_017008811.1 | 33.5% | 30.6% | (many diffs) |
| 2 | human | 8999 | CDKL2 | cyclin dependent kinase like 2 | NM_003948.5 | 33.1% | 30.8% | (many diffs) |
| 3 | human | 8999 | CDKL2 | cyclin dependent kinase like 2 | XM_017008810.1 | 33.1% | 30.8% | (many diffs) |
| 4 | human | 8999 | CDKL2 | cyclin dependent kinase like 2 | XM_006714406.2 | 30.5% | 28.6% | (many diffs) |
| 5 | human | 8999 | CDKL2 | cyclin dependent kinase like 2 | NM_001330724.2 | 28.3% | 25.9% | (many diffs) |
| 6 | human | 8999 | CDKL2 | cyclin dependent kinase like 2 | XM_017008809.1 | 28.3% | 25.9% | (many diffs) |
| 7 | human | 8999 | CDKL2 | cyclin dependent kinase like 2 | XR_001741344.1 | 10.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 591
- ORF length:
- 522
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctatatt tattttcatt ttagtaaact gtttcacgtt agacctgtaa 121 cccaaggaga tgtgtataga gctgaaactG AAGAAAGAAA AACACTTGTG GTACAGGATA 181 CCAATGCTGA TCCCAAAATT AAGGATTATA AACTATTTAA AATAAAAGGC TCAAAAATTG 241 ATGGAGAAAA AGCTGAAAAA GGCAATAGAG CTTCAAATGC CAGCTGTCTC CATGACAGTA 301 GGACAAGCCA CAACAAAATA GTGCCTTCAA CAAGCCTCAA AGACTGCAGC AATGTCAGCG 361 TGGACCACAC AAGGAATCCA AGCGTGGCAA TTCCCCCACT TACACACAAT CTTTCTGCAG 421 TTGCTCCCAG CATTAATTCT GGAATGGGGA CTGAGACTAT ACCAATTCAG GGTTACAGAG 481 TGGATGAGAA AACTAAGAAG TGTTCTATTC CATTTGTTAA ACCGAACAGA CATTCCCCAT 541 CAGGCATTTA TAACATTAAT GTGACCACAT TAGTATCAGG ACCTCCCCTG TCAGATGATT 601 CAGGGGCTGA TTTGCCTCAA ATGGAACACC AGCACTTGCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 ATTCCAGGTC AAGTCGTGAC ATCTGACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt