Transcript: Human NM_001330724.2

Homo sapiens cyclin dependent kinase like 2 (CDKL2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-21
Taxon:
Homo sapiens (human)
Gene:
CDKL2 (8999)
Length:
4935
CDS:
517..2229

Additional Resources:

NCBI RefSeq record:
NM_001330724.2
NBCI Gene record:
CDKL2 (8999)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148165 AGTGGTGATAGATTTAGCAA pXPR_003 AGG 790 46% 6 0.9392 CDKL2 CDKL2 77588
2 BRDN0001149437 CTGATTATGTGGCAACCCGA pXPR_003 TGG 492 29% 4 0.8145 CDKL2 CDKL2 77587
3 BRDN0001145372 CTGGTAACTGAAATGTTCAT pXPR_003 GGG 587 34% 5 -0.1134 CDKL2 CDKL2 77589
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001330724.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000355562 GATGTGTTTAGGTAATCTAAT pLKO_005 1161 CDS 100% 13.200 18.480 N CDKL2 n/a
2 TRCN0000338224 GCGAGAAATCAAGTTACTAAA pLKO_005 663 CDS 100% 13.200 18.480 N CDKL2 n/a
3 TRCN0000350964 CGTTGTCAAGCTATGCGATTT pLKO_005 930 CDS 100% 10.800 15.120 N CDKL2 n/a
4 TRCN0000000728 TGCGAGAAATCAAGTTACTAA pLKO.1 662 CDS 100% 5.625 7.875 N CDKL2 n/a
5 TRCN0000000725 CAGTAGGACAAGCCACAACAA pLKO.1 1656 CDS 100% 4.950 6.930 N CDKL2 n/a
6 TRCN0000199431 CCATTGGTTGTCTGGTAACTG pLKO.1 1076 CDS 100% 4.950 6.930 N CDKL2 n/a
7 TRCN0000378478 GGGAGGTTTATACTGATTATG pLKO_005 980 CDS 100% 13.200 9.240 N CDKL2 n/a
8 TRCN0000338223 TCACGTCCACCGATGCTATTT pLKO_005 2459 3UTR 100% 13.200 9.240 N CDKL2 n/a
9 TRCN0000418915 TTACTGTTAGGAGATTGTATA pLKO_005 2535 3UTR 100% 13.200 9.240 N CDKL2 n/a
10 TRCN0000000727 CCATCAGGCATTTATAACATT pLKO.1 1897 CDS 100% 5.625 3.938 N CDKL2 n/a
11 TRCN0000000726 GAGTTATGGAATGGTGATGAA pLKO.1 555 CDS 100% 4.950 3.465 N CDKL2 n/a
12 TRCN0000338222 GAGTTATGGAATGGTGATGAA pLKO_005 555 CDS 100% 4.950 3.465 N CDKL2 n/a
13 TRCN0000000724 GAACTGGATGATGCTCTTGCA pLKO.1 2246 3UTR 100% 2.640 1.848 N CDKL2 n/a
14 TRCN0000195050 CTGATATTGATCAGCTATATC pLKO.1 1133 CDS 100% 1.320 0.924 N CDKL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001330724.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000475406 TTCCAGGTCAAGTCGTGACATCTG pLX_317 84.2% 28.3% 25.9% V5 (many diffs) n/a
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