Construct: ORF TRCN0000475410
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003146.1_s317c1
- Derived from:
- ccsbBroadEn_01735
- DNA Barcode:
- TCCCCCCACGCGCCCCCGCCATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2D1 (7321)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475410
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7321 | UBE2D1 | ubiquitin conjugating enzym... | NM_003338.5 | 100% | 100% | |
| 2 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | NM_015983.4 | 77.5% | 91.8% | (many diffs) |
| 3 | human | 7321 | UBE2D1 | ubiquitin conjugating enzym... | NM_001204880.1 | 73.9% | 72.7% | 0_1ins111;2_3insGGG;4A>C |
| 4 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_001300795.1 | 64.7% | 70.7% | (many diffs) |
| 5 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | XM_017008584.2 | 64.7% | 70.7% | (many diffs) |
| 6 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_024446796.1 | 57.3% | 68% | (many diffs) |
| 7 | mouse | 216080 | Ube2d1 | ubiquitin-conjugating enzym... | NM_145420.2 | 89.3% | 100% | (many diffs) |
| 8 | mouse | 216080 | Ube2d1 | ubiquitin-conjugating enzym... | XM_006513478.2 | 81.4% | 89.3% | (many diffs) |
| 9 | mouse | 216080 | Ube2d1 | ubiquitin-conjugating enzym... | XM_006513479.2 | 66.6% | 73.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctgaagagg attcagaaag aattgagtga tctacagcgc gatccacctg 121 ctcactgttc agctggacct gtgggagatg acttgttcca ctggcaagcc actattatgg 181 ggcctccTGA TAGCGCATAT CAAGGTGGAG TCTTCTTTCT CACTGTACAT TTTCCGACAG 241 ATTATCCTTT TAAACCACCA AAGATTGCTT TCACAACAAA AATTTACCAT CCAAACATAA 301 ACAGTAATGG AAGTATTTGT CTCGATATTC TGAGGTCACA ATGGTCACCA GCTCTGACTG 361 TATCAAAAGT TTTATTGTCC ATATGTTCTC TACTTTGTGA TCCTAATCCA GATGACCCCT 421 TAGTACCAGA TATTGCACAA ATCTATAAAT CAGACAAAGA AAAATACAAC AGACATGCAA 481 GAGAATGGAC TCAGAAATAT GCAATGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCCCCCCA 661 CGCGCCCCCG CCATCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt