Construct: ORF TRCN0000475444
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006249.1_s317c1
- Derived from:
- ccsbBroadEn_07649
- DNA Barcode:
- CAAGGGATGGAAGCCACCAATCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDLIM5 (10611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475444
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001011516.2 | 99.8% | 100% | 468G>A |
| 2 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001011515.2 | 89.8% | 87.6% | (many diffs) |
| 3 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001256427.2 | 41.3% | 36.3% | (many diffs) |
| 4 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001011513.4 | 40.3% | 35.2% | (many diffs) |
| 5 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_006457.5 | 33.7% | 29.5% | (many diffs) |
| 6 | human | 10611 | PDLIM5 | PDZ and LIM domain 5 | NM_001256426.1 | 31.7% | 29.2% | (many diffs) |
| 7 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_001190853.1 | 90.1% | 94.3% | (many diffs) |
| 8 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_001190857.1 | 81.1% | 83.3% | (many diffs) |
| 9 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501751.2 | 81.1% | 83.3% | (many diffs) |
| 10 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_022554.3 | 80.1% | 83.2% | (many diffs) |
| 11 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501750.2 | 80.1% | 83.2% | (many diffs) |
| 12 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | NM_019809.3 | 57% | 57.2% | (many diffs) |
| 13 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501746.1 | 31% | 27.4% | (many diffs) |
| 14 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_017319659.1 | 30.2% | 29.1% | (many diffs) |
| 15 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501743.1 | 27.9% | 27% | (many diffs) |
| 16 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501742.1 | 26.4% | 25.1% | (many diffs) |
| 17 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501741.1 | 25.9% | 25.3% | (many diffs) |
| 18 | mouse | 56376 | Pdlim5 | PDZ and LIM domain 5 | XM_006501738.1 | 24.8% | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caactacagt gtgtcactgg ttggcccagc tccttggggt ttccggctgc 121 agggcggtaa ggatttcaac atgcctctga caatctctag tctaaaagat ggcggcaagg 181 cagcccaggc aaatgtaaga ataggcgatg tggttctcag cattgatgga ataaatgcac 241 aaggaatgac tcatcttgaa gcccagaata agattaaggg ttgtacaggc tctttgaata 301 tgactctgca aagagcatct gctgcaccca agcctGAGCC GGTTCCTGTT CAAAAGAAAA 361 CACAAGTGAC AAATAACCCT GGCACTGTGA AAATCCCACC TAAACGCCCA CCAAGAAAAC 421 ACATTGTGGA GCGCTATACA GAGTTTTATC ATGTACCCAC TCACAGTGAT GCCAGCAAGA 481 AGAGACTGAT TGAGGATACT GAAGACTGGC GTCCAAGGAC TGGAACAACT CAATCTCGCT 541 CTTTCCGAAT CCTTGCCCAG ATCACTGGGA CTGAACATTT GAAAGAATCT GAAGCCGATA 601 ATACAAAGAA GGCAAAGGAA AAGATACCCC TTCACGTCTT TAGTCCCAAA TACACAAAAT 661 TACGTGACTG GCACCATGAA GTTTCAGCAC GTGCTCTTAA CGTACAGTGC CCAACTTTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGACAAGGGA TGGAAGCCAC CAATCTAACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt