Construct: ORF TRCN0000475460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016023.1_s317c1
- Derived from:
- ccsbBroadEn_05645
- DNA Barcode:
- CGTATAAGACGAATGCCGAGTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAMD12 (401474)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475460
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401474 | SAMD12 | sterile alpha motif domain ... | NM_207506.2 | 100% | 100% | |
| 2 | human | 401474 | SAMD12 | sterile alpha motif domain ... | NM_001101676.2 | 77.5% | 75.6% | (many diffs) |
| 3 | human | 401474 | SAMD12 | sterile alpha motif domain ... | NM_001363274.2 | 76.4% | 77.1% | 464_467delGGCA;468_469ins139 |
| 4 | human | 401474 | SAMD12 | sterile alpha motif domain ... | NM_001349811.2 | 72.6% | 70.2% | (many diffs) |
| 5 | human | 401474 | SAMD12 | sterile alpha motif domain ... | NR_146234.2 | 40% | (many diffs) | |
| 6 | human | 401474 | SAMD12 | sterile alpha motif domain ... | NR_109794.2 | 6.3% | (many diffs) | |
| 7 | mouse | 320679 | Samd12 | sterile alpha motif domain ... | XM_006521080.3 | 78.3% | 79.6% | (many diffs) |
| 8 | mouse | 320679 | Samd12 | sterile alpha motif domain ... | XM_011245660.2 | 75% | 70.5% | (many diffs) |
| 9 | mouse | 320679 | Samd12 | sterile alpha motif domain ... | XM_006521081.3 | 73.6% | 74.1% | (many diffs) |
| 10 | mouse | 320679 | Samd12 | sterile alpha motif domain ... | NM_177225.4 | 70.4% | 73.2% | (many diffs) |
| 11 | mouse | 320679 | Samd12 | sterile alpha motif domain ... | XM_006521084.3 | 69.8% | 73.6% | (many diffs) |
| 12 | mouse | 320679 | Samd12 | sterile alpha motif domain ... | XM_006521085.3 | 65.7% | 67.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 672
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgtggaa gctctccact gtggtttgaa tccacggggt attgatcacc 121 ctgcccatgc tgaaggtatt aaactgcaaa ttgaaggtga aggtgtggaa tctcaatcca 181 ttaaaaataa aaatttccag aaggtgcctg accagaaagg aactcccaag cgactgcagg 241 cagaagctga gacggctaag tcagctacgg tgaagctatc taaaccggtg gctctatgga 301 cccagcagga tgtctgcaag tggttgaaga aacattgtcc gaatcagtat cagatctaca 361 gtgagtcatt caaacagcat gacataactg ggcgagccct gctgagactt actgacaaaa 421 agctcgagcg aatggggatt gcccaggaga acctccggca gcacatctta caacaggtgc 481 tccagctgaa ggtgcgagaa gaagtcagaa atctacagtt actcacacaa ggtaccctat 541 tgcttcctga tgggtggatg gatggggaga ttagaagaaa gaccacctta ctattaGGAC 601 AGACAGGAGT CAGGGAGAAT TTGTTATTGT TTCTTCACAG AATTTCCATC ATAGAAAATA 661 GTATACAGAT TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 721 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 781 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGT ATAAGACGAA TGCCGAGTAG 841 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t