Construct: ORF TRCN0000475472
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001221.1_s317c1
- Derived from:
- ccsbBroadEn_07474
- DNA Barcode:
- CCGCGACCCTACGAAGCCATTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECE2 (9718)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475472
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9718 | ECE2 | endothelin converting enzyme 2 | NM_001037324.3 | 99.9% | 99.8% | 630C>T;959T>C |
| 2 | human | 9718 | ECE2 | endothelin converting enzyme 2 | NM_001100121.2 | 96.1% | 96% | 40_126del;717C>T;1046T>C |
| 3 | human | 9718 | ECE2 | endothelin converting enzyme 2 | NM_001100120.2 | 90.6% | 90.6% | 40_264del;855C>T;1184T>C |
| 4 | human | 110599583 | EEF1AKMT4-ECE2 | EEF1AKMT4-ECE2 readthrough | NM_014693.4 | 82.7% | 81.8% | (many diffs) |
| 5 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | NM_177942.1 | 88.7% | 92.7% | (many diffs) |
| 6 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | NM_139293.2 | 85.6% | 89.2% | (many diffs) |
| 7 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_006521693.3 | 85.1% | 79.7% | (many diffs) |
| 8 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_006521691.3 | 82.2% | 76.8% | (many diffs) |
| 9 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_006521696.3 | 80.3% | 82.3% | (many diffs) |
| 10 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_006521692.3 | 80.2% | 80.1% | (many diffs) |
| 11 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_006521697.3 | 78.5% | 81.8% | (many diffs) |
| 12 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_006521694.3 | 75.9% | 78.2% | (many diffs) |
| 13 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_006521695.3 | 75.8% | 78% | (many diffs) |
| 14 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | NM_177940.1 | 73.4% | 76.4% | (many diffs) |
| 15 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | NM_177941.1 | 71.6% | 74% | (many diffs) |
| 16 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_017316848.1 | 64% | 59% | (many diffs) |
| 17 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_006521698.3 | 60.3% | 62.2% | (many diffs) |
| 18 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_006521700.3 | 56.4% | 51.1% | (many diffs) |
| 19 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | XM_011245802.2 | 56.4% | 51.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2277
- ORF length:
- 2208
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcactat gaacgtcgcg ctgcaggagc tgggagctgg cagcaacgtg ggattccaga 121 aggggacaag acagctgtta ggctcacgca cgcagctgga gctggtctta gcaggtgcct 181 ctctactgct ggctgcactg cttctgggct gccttgtggc cctaggggtc cagtaccaca 241 gagacccatc ccacagcacc tgccttacag aggcctgcat tcgagtggct ggaaaaatcc 301 tggagtccct ggaccgaggg gtgagcccct gtgaggactt ttaccagttc tcctgtgggg 361 gctggattcg gaggaacccc ctgcccgatg ggcgttctcg ctggaacacc ttcaacagcc 421 tctgggacca aaaccaggcc atactgaagc acctgcttga aaacaccacc ttcaactcca 481 gcagtgaagc tgagcagaag acacagcgct tctacctatc ttgcctacag gtggagcgca 541 ttgaggagct gggagcccag ccactgagag acctcattga gaagattggt ggttggaaca 601 ttacggggcc ctgggaccag gacaacttta tggaggtgtt gaaggcagta gcagggacct 661 acagggccac cccattcttc accgtctaca tcagtgctga ctctaagagt tccaacagca 721 atgttatcca ggtggaccag tctgggctct ttctgccctc tcgggattac tacttaaaca 781 gaactgccaa tgagaaagtg ctcactgcct atctggatta catggaggaa ctggggatgc 841 tgctgggtgg gcggcccacc tccacgaggg agcagatgca gcaggtgctg gagttggaga 901 tacagctggc caacatcaca gtgccccagg accagcggcg cgacgaggag aagatctacc 961 acaagatgag catttcggag ctgcaggctc tggcgccctc catggactgg cttgagttcc 1021 tgtcttcctt gctgtcacca ttggagttga gtgactctga gcctgtggtg gtgtatggga 1081 tggattattt gcagcaggtg tcagagctca tcaaccgcac ggaaccaagc atcctgaaca 1141 attacctgat ctggaacctg gtgcaaaaga caacctcaag cctggaccga cgctttgagt 1201 ctgcacaaga gaagctgctg gagaccctct atggcactaa gaagtcctgt gtgccgaggt 1261 ggcagacctg catctccaac acggatgacg cccttggctt tgctttgggg tccctcttcg 1321 tgaaggccac gtttgaccgg caaagcaaag aaattgcaga ggggatgatc agcgaaatcc 1381 ggaccgcatt tgaggaggcc ctgggacagc tggtttggat ggatgagaag acccgccagg 1441 cagccaagga gaaagcagat gccatctatg atatgattgg tttcccagac tttatcctgg 1501 agcccaaaga gctggatgat gtttatgacg ggtacgaaat ttctgaagat tctttcttcc 1561 aaaacatgtt gaatttgtac aacttctctg ccaaggttat ggctgaccag ctccgcaagc 1621 ctcccagccg agaccagtgg agcatgaccc cccagacagt gaatgcctac taccttccaa 1681 ctaagaatga gatcgtcttc cccgctggca tcctgcaggc ccccttctat gcccgcaacc 1741 accccaaggc cctgaacttc ggtggcatcg gtgtggtcat gggccatgag ttgacgcatg 1801 cctttgatga ccaagggcgc gagtatgaca aagaagggaa cctgcggccc tggtggcaga 1861 atgagtccct ggcagccttc cggaaccaca cggcctgcat ggaggaacag tacaatcaat 1921 accaggtcaa tggggagagg ctcaacggcc gccagacgct gggggagaac attgctgaca 1981 acggggggcT GAAGGCTGCC TACAATGCTT ACAAAGCATG GCTGAGAAAG CATGGGGAGG 2041 AGCAGCAACT GCCAGCCGTG GGGCTCACCA ACCACCAGCT CTTCTTCGTG GGATTTGCCC 2101 AGGTGTGGTG CTCGGTCCGC ACACCAGAGA GCTCTCACGA GGGGCTGGTG ACCGACCCCC 2161 ACAGCCCTGC CCGCTTCCGC GTGCTGGGCA CTCTCTCCAA CTCCCGTGAC TTCCTGCGGC 2221 ACTTCGGCTG CCCTGTCGGC TCCCCCATGA ACCCAGGGCA GCTGTGTGAG GTGTGGTTGC 2281 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2341 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2401 ATATATCTTG TGGAAAGGAC GACCGCGACC CTACGAAGCC ATTGCCACGC GTTAAGTCga 2461 caatcaacct ctggattaca aaatttgtga aagatt