Construct: ORF TRCN0000475475
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014212.1_s317c1
- Derived from:
- ccsbBroadEn_13977
- DNA Barcode:
- CGCGAGGCGGATAATTATTTAATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- UBE2D3 (7323)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475475
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_003340.6 | 99.3% | 7delC;357A>G;367A>C | |
| 2 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181886.3 | 99.3% | 7delC;357A>G;367A>C | |
| 3 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181887.2 | 99.3% | 7delC;357A>G;367A>C | |
| 4 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181888.3 | 99.3% | 7delC;357A>G;367A>C | |
| 5 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181889.2 | 99.3% | 7delC;357A>G;367A>C | |
| 6 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181890.2 | 99.3% | 7delC;357A>G;367A>C | |
| 7 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181891.3 | 99.3% | 7delC;357A>G;367A>C | |
| 8 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | XM_024454202.1 | 99.3% | 7delC;357A>G;367A>C | |
| 9 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181893.2 | 95.7% | (many diffs) | |
| 10 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181892.3 | 95.7% | (many diffs) | |
| 11 | human | 7322 | UBE2D2 | ubiquitin conjugating enzym... | NM_003339.3 | 85.9% | (many diffs) | |
| 12 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_001300795.1 | 80% | 0_1ins86;270A>G;280A>C | |
| 13 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | XM_017008584.2 | 80% | 0_1ins86;270A>G;280A>C | |
| 14 | human | 7322 | UBE2D2 | ubiquitin conjugating enzym... | XM_017009820.1 | 73.4% | (many diffs) | |
| 15 | human | 7322 | UBE2D2 | ubiquitin conjugating enzym... | NM_181838.1 | 70.4% | (many diffs) | |
| 16 | mouse | 66105 | Ube2d3 | ubiquitin-conjugating enzym... | NM_025356.4 | 97.7% | (many diffs) | |
| 17 | mouse | 66105 | Ube2d3 | ubiquitin-conjugating enzym... | XM_006501873.2 | 97.7% | (many diffs) | |
| 18 | mouse | 66105 | Ube2d3 | ubiquitin-conjugating enzym... | XM_006501874.2 | 97.7% | (many diffs) | |
| 19 | mouse | 66105 | Ube2d3 | ubiquitin-conjugating enzym... | XM_006501875.2 | 97.7% | (many diffs) | |
| 20 | mouse | 56550 | Ube2d2a | ubiquitin-conjugating enzym... | NM_019912.2 | 85% | (many diffs) | |
| 21 | mouse | 56550 | Ube2d2a | ubiquitin-conjugating enzym... | XM_006526120.2 | 70.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 72
- ORF length:
- 6
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtgaaacgga ttaataagga acttagtgat ttggcccgtg accctccagc 121 acaatgttct gcaggtccag ttggggatga tatgtttcat tggcaagcca caattatggg 181 accTAATGAC AGCCCATATC AAGGCGGTGT ATTCTTTTTG ACAATTCATT TTCCTACAGA 241 CTACCCCTTC AAACCACCTA AGGTTGCATT TACAACAAGA ATTTATCATC CAAATATTAA 301 CAGTAATGGC AGCATTTGTC TCGATATTCT AAGATCACAG TGGTCGCCTG CTTTAACAAT 361 TTCTAAAGTT CTTTTATCCA TTTGTTCACT GCTATGTGAT CCAAACCCAG ATGACCCCCT 421 GGTGCCAGAG CTTGCACGGA TCTATAAAAC AGACAGAGAT AAGTACAACA GAATATCTCG 481 GGAATGGACT CAGAAGTATG CCATGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 541 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 601 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGCGAGGCG 661 GATAATTATT TAATTACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 721 agatt