Construct: ORF TRCN0000475485
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007995.1_s317c1
- Derived from:
- ccsbBroadEn_08562
- DNA Barcode:
- CTGGCCTTTGCCATGGGTGGATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX27 (55661)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475485
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55661 | DDX27 | DEAD-box helicase 27 | NM_017895.7 | 99.9% | 99.8% | 2111T>C |
| 2 | human | 55661 | DDX27 | DEAD-box helicase 27 | NM_001348187.1 | 96.2% | 96.1% | 1122_1214del;2204T>C |
| 3 | mouse | 228889 | Ddx27 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_153065.3 | 84.2% | 88.8% | (many diffs) |
| 4 | mouse | 228889 | Ddx27 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_006499422.1 | 81.5% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2457
- ORF length:
- 2388
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtacttgcg caaagacgac gaggaggctg cgaaaagtta agggccggac 121 cgcaggctgt gctcgcttcc ggaagtggct tctgcgacaa catgcttgcg gacctcggct 181 taatcggaac cataggcgag gatgacgagg tgccggtgga gcccgagtct gactccgggg 241 acgaggaaga ggaggggccc attgtgctgg gcagacgaca aaaagctttg gggaagaacc 301 gcagtgctga tttcaaccct gatttcgttt tcactgagaa ggaggggacg tacgatggca 361 gctgggccct ggctgatgtc atgagccaac tcaagaagaa gagggcagcc actacattag 421 atgagaagat tgagaaagtt cgaaagaaaa ggaaaacaga ggataaagaa gccaagtctg 481 ggaagttgga aaaggagaaa gaagcaaagg aaggctctga accaaaggag caggaagacc 541 ttcaagagaa tgatgaggaa ggctcagaag atgaagcctc ggagactgac tactcatcag 601 ctgatgagaa catcctcacc aaagcagata cactcaaagt aaaggatcgg aagaagaaga 661 agaagaaagg acaggaagca ggaggatttt ttgaagatgc atctcagtac gatgaaaacc 721 tctcgttcca ggacatgaac ctttcccgcc ctcttctgaa ggccattaca gccatgggct 781 tcaagcagcc caccccgatc cagaaggcgt gcatacctgt gggtctattg gggaaggaca 841 tctgtgcctg tgcagccact gggacaggta aaactgccgc ctttgccctg cctgttttgg 901 agcgtctgat ttataaaccc cgccaggctc cagtcacccg cgtgctggtg ctagtgccca 961 cccgagagct gggcatccag gtgcactctg tcaccagaca gctggcccag ttctgcaaca 1021 tcaccacctg cctggctgtg ggcggcttgg atgtgaagtc tcaggaagca gctcttcggg 1081 cagcgcctga catcctcatc gccaccccag gccggctcat cgatcacctc cacaactgcc 1141 cttccttcca cctgagcagc atcgaggtgc tcatcctgga cgaggctgac aggatgctgg 1201 atgagtactt tgaggagcag atgaaggaga tcatccgaat gtgttcccac caccgccaga 1261 ccatgctctt ctcggccacc atgacagacg aggtgaaaga tctggcttct gtctccttga 1321 agaatcctgt ccggatattt gtgaacagca acacagatgt ggctcccttc ctgcggcagg 1381 agttcatccg gatccggcct aatcgtgaag gagaccggga agccatcgtg gcagctttgt 1441 tgacgaggac cttcactgac catgtgatgc tgttcacgca aaccaagaag caggcccacc 1501 gcatgcacat cctcctgggg ctcatggggc tgcaggtggg tgagctccat ggcaacttgt 1561 cacagacgca gcggctggag gccctccggc gttttaagga tgaacagatt gacatcctcg 1621 tggccactga tgtggcagcc cgtggacttg acattgaggg ggtcaaaacg gtaatcaact 1681 tcacaatgcc taataccatc aaacattatg tccaccgggt ggggcgaaca gcacgtgctg 1741 gcagggctgg gcgctcagtc tctctggtgg gagaagatga gcggaagatg ctgaaggaga 1801 ttgtaaaagc tgccaaggcc cctgtgaagg ccaggatact tccccaagat gtcatcctca 1861 aattccggga caagattgag aaaatggaga aagatgtgta tgcagttctg cagctagagg 1921 cggaggaaaa agagatgcag cagtcagaag cccagatcaa tacagcaaag cggctcctgg 1981 agaaggggaa ggaggcagtg gtccaagagc ccgagaggag ctggttccag accaaagaag 2041 agaggaagaa ggagaaaatt gccaaagctc tgcaggaatt tgacttggcc ttaagagGAA 2101 AGAAGAAAAG GAAGAAGTTT ATGAAGGATG CCAAAAAAAA GGGGGAGATG ACAGCAGAGG 2161 AAAGGTCTCA GTTTGAAACC CTCAAGGCGC AGATGTTTGC TGAACGGCTA GCGAAGAGGA 2221 ATCGCAGAGC CAAGCGGGCC CGAGCAATGC CCGAGGAGGA GCCAGTGAGA GGTCCTGCCA 2281 AGAAGCAAAA GCAGGGGAAG AAATCTGTAT TTGATGAAGA ACTCACCAAC ACAAGCAAGA 2341 AGGCCCTGAA ACAGTATCGA GCTGGCCCTT CCTTTGAAGA AAGGAAACAG TTGGGCTTGC 2401 CCCACCAGAG ACGAGGAGGA AACTTTAAAT CTAAATCCAG ATACAAGAGG AGGAAGTTGC 2461 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2521 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2581 ATATATCTTG TGGAAAGGAC GACTGGCCTT TGCCATGGGT GGATAAACGC GTTAAGTCga 2641 caatcaacct ctggattaca aaatttgtga aagatt