Construct: ORF TRCN0000475667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013303.1_s317c1
- Derived from:
- ccsbBroadEn_02726
- DNA Barcode:
- GCGGTCCAATCCCCATATAGTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC35D1 (23169)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23169 | SLC35D1 | solute carrier family 35 me... | NM_015139.3 | 100% | 100% | |
| 2 | human | 23169 | SLC35D1 | solute carrier family 35 me... | XM_006710478.2 | 92.9% | 92.9% | 728_808del |
| 3 | human | 23169 | SLC35D1 | solute carrier family 35 me... | XM_011541070.2 | 92.9% | 92.9% | 728_808del |
| 4 | human | 23169 | SLC35D1 | solute carrier family 35 me... | XR_001737057.2 | 16.1% | (many diffs) | |
| 5 | human | 23169 | SLC35D1 | solute carrier family 35 me... | XR_001737058.2 | 14.3% | 1_403del;1278_2243del;2435_7399del | |
| 6 | mouse | 242585 | Slc35d1 | solute carrier family 35 (U... | XM_006503080.3 | 92.1% | 97.1% | (many diffs) |
| 7 | mouse | 242585 | Slc35d1 | solute carrier family 35 (U... | NM_177732.4 | 80% | 83.6% | (many diffs) |
| 8 | mouse | 242585 | Slc35d1 | solute carrier family 35 (U... | XM_006503079.3 | 78.5% | 82.1% | (many diffs) |
| 9 | mouse | 242585 | Slc35d1 | solute carrier family 35 (U... | XR_376320.3 | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1134
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggaagtt catagacgtc agcatgctcg ggttaaagga gaagcccccg 121 cgaaatcctc cacactccga gatgaggagg agctggggat ggcgtcggcc gaaacgctga 181 ccgtgtttct gaagctgctg gccgccggct tttacggcgt gagctccttc ctgatcgtgg 241 tggtgaataa gagcgtgctc accaattaca gatttccctc ctcactatgt gttggacttg 301 gccagatggt ggccacagtg gcagttctct gggtgggaaa ggcgctcaga gtagtcaagt 361 ttcctgacct tgacagaaat gtacctcgaa agacgtttcc actacctcta ctatattttg 421 ggaaccaaat cacgggactg ttcagcacaa agaaactgaa cttgccaatg tttacagttc 481 tgagaaggtt ctccatcctg tttacaatgt ttgctgaagg agttttactc aagaagactt 541 tttcttgggg tattaaaatg actgtatttg caatgattat tggagccttt gtagctgcca 601 gctctgactt ggcatttgat ctggaaggat atgcttttat tctgataaac gatgtcctaa 661 cagcagcaaa tggtgcatac gtaaaacaaa aattagattc aaaagagctg ggaaaatatg 721 gactgctcta ttacaatgca ctgttcatga ttctgcccac cctggccatt gcgtatttca 781 caggagatgc acaaaaggct gtggagtttg aaggctgggc tgacaccctc tttcttctgc 841 agttcaccct ctcctgtgtg atggggttta tcttaatgta cgccacagta ctctgcacgc 901 agtataattc tgctcttaca actacaatag ttggctgtat taagaatata ttaataactt 961 atattggaat ggtctttggt ggagattata ttttcacgtg gacaaacttc attggtttaa 1021 atatcagcat tgctgggagc ctgGTATATT CCTATATCAC TTTCACTGAA GAGCAGCTGA 1081 GCAAACAGTC AGAGGCTAAT AACAAGCTGG ACATTAAGGG GAAAGGAGCA GTGTTGCCAA 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAG CGGTCCAATC CCCATATAGT TTTACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att