Construct: ORF TRCN0000475701
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013157.1_s317c1
- Derived from:
- ccsbBroadEn_06963
- DNA Barcode:
- TTGTACCCCTCCATCTAGGCTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC8A3 (6547)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475701
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | NM_182936.3 | 99.8% | 100% | 468C>G |
| 2 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | NM_001130417.3 | 95.1% | 95.3% | 1_42del;510C>G |
| 3 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | XM_006720240.3 | 93.3% | 93.4% | 1_60del;528C>G |
| 4 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | XM_017021611.1 | 91.2% | 91.3% | 1_81del;549C>G |
| 5 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | NM_182932.3 | 30.7% | 30.8% | 1_1911del;2379C>G |
| 6 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | XM_017021608.1 | 30.7% | 30.8% | 1_1911del;2379C>G |
| 7 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | NM_058240.4 | 30.6% | 30.7% | 1_1920del;2388C>G |
| 8 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | XM_017021609.1 | 30.6% | 30.7% | 1_1911del;2109_2110insGCA;2376C>G |
| 9 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | NM_033262.4 | 30.6% | 30.7% | 1_1923del;2391C>G |
| 10 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | NM_183002.3 | 30.6% | 30.6% | 1_1929del;2397C>G |
| 11 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | XM_017021606.1 | 30.6% | 30.6% | 1_1929del;2397C>G |
| 12 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | XM_017021607.1 | 30.4% | 30.5% | 1_1929del;2127_2128insGCA;2394C>G |
| 13 | human | 6547 | SLC8A3 | solute carrier family 8 mem... | NR_104122.2 | 16.9% | 1_2442del;2910C>G;3295_5029del | |
| 14 | mouse | 110893 | Slc8a3 | solute carrier family 8 (so... | XM_006515414.2 | 84.9% | 91.4% | (many diffs) |
| 15 | mouse | 110893 | Slc8a3 | solute carrier family 8 (so... | XM_006515413.3 | 75.5% | 81.2% | (many diffs) |
| 16 | mouse | 110893 | Slc8a3 | solute carrier family 8 (so... | NM_001167920.1 | 28% | 30.1% | (many diffs) |
| 17 | mouse | 110893 | Slc8a3 | solute carrier family 8 (so... | XM_006515411.3 | 27.8% | 29.9% | (many diffs) |
| 18 | mouse | 110893 | Slc8a3 | solute carrier family 8 (so... | XM_006515412.3 | 27.8% | 29.9% | (many diffs) |
| 19 | mouse | 110893 | Slc8a3 | solute carrier family 8 (so... | NM_080440.3 | 27.8% | 29.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 921
- ORF length:
- 852
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagaagag gaggccaaga ggatagcaga gatgggaaag ccagtattgg 121 gtgaacaccc caaactagaa gtcatcattg aagagtccta tgagttcaag actacggtgg 181 acaaactgat caagaagaca aacctggcct tggttgtggg gacccattcc tggagggacc 241 agttcatgga ggccatcacc gtcagtgcag caggggatga ggatgaggat gaatccgggg 301 aggagaggct gccctcctgc tttgactacg tcatgcactt cctgactgtc ttctggaagg 361 tgctgtttgc ctgtgtgccc cccacagagt actgccacgg ctgggcctgc ttcgccgtct 421 ccatcctcat cattggcatg ctcaccgcca tcattgggga cctggcctcg cacttcggct 481 gcaccattgg tctcaaagat tcagtcacag ctgttgtttt cgtggcattt ggcacgtctg 541 tcccagatac gtttgccagc aaagctgctg cccTCCAGGA TGTATATGCA GACGCCTCCA 601 TTGGCAACGT GACGGGCAGC AACGCCGTCA ATGTCTTCCT GGGCATCGGC CTGGCCTGGT 661 CCGTGGCCGC CATCTACTGG GCTCTGCAGG GACAGGAGTT CCACGTGTCG GCCGGCACAC 721 TGGCCTTCTC CGTCACCCTC TTCACCATCT TTGCATTTGT CTGCATCAGC GTGCTCTTGT 781 ACCGAAGGCG GCCGCACCTG GGAGGGGAGC TTGGTGGCCC CCGTGGCTGC AAGCTCGCCA 841 CAACATGGCT CTTTGTGAGC CTGTGGCTCC TCTACATACT CTTTGCCACA CTAGAGGCCT 901 ATTGCTACAT CAAGGGGTTC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 961 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1021 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTGT ACCCCTCCAT 1081 CTAGGCTCTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt