Construct: ORF TRCN0000475807
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002794.1_s317c1
- Derived from:
- ccsbBroadEn_01020
- DNA Barcode:
- TGAGCATTCACCCTCACACGGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MME (4311)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475807
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4311 | MME | membrane metalloendopeptidase | NM_000902.4 | 100% | 100% | |
| 2 | human | 4311 | MME | membrane metalloendopeptidase | NM_001354642.1 | 100% | 100% | |
| 3 | human | 4311 | MME | membrane metalloendopeptidase | NM_001354643.1 | 100% | 100% | |
| 4 | human | 4311 | MME | membrane metalloendopeptidase | NM_007287.3 | 100% | 100% | |
| 5 | human | 4311 | MME | membrane metalloendopeptidase | NM_007288.3 | 100% | 100% | |
| 6 | human | 4311 | MME | membrane metalloendopeptidase | XM_006713647.4 | 100% | 100% | |
| 7 | human | 4311 | MME | membrane metalloendopeptidase | XM_011512856.2 | 100% | 100% | |
| 8 | human | 4311 | MME | membrane metalloendopeptidase | XM_011512857.2 | 100% | 100% | |
| 9 | human | 4311 | MME | membrane metalloendopeptidase | NM_001354644.1 | 9.8% | 8.8% | (many diffs) |
| 10 | mouse | 17380 | Mme | membrane metallo endopeptidase | NM_001289462.1 | 90.9% | 94.1% | (many diffs) |
| 11 | mouse | 17380 | Mme | membrane metallo endopeptidase | NM_001289463.1 | 90.9% | 94.1% | (many diffs) |
| 12 | mouse | 17380 | Mme | membrane metallo endopeptidase | NM_008604.4 | 90.9% | 94.1% | (many diffs) |
| 13 | mouse | 17380 | Mme | membrane metallo endopeptidase | XM_006501095.3 | 90.9% | 94.1% | (many diffs) |
| 14 | mouse | 17380 | Mme | membrane metallo endopeptidase | XM_006501098.3 | 90.9% | 94.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2319
- ORF length:
- 2250
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaagtca gaaagtcaga tggatataac tgatatcaac actccaaagc 121 caaagaagaa acagcgatgg actccactgg agatcagcct ctcggtcctt gtcctgctcc 181 tcaccatcat agctgtgaca atgatcgcac tctatgcaac ctacgatgat ggtatttgca 241 agtcatcaga ctgcataaaa tcagctgctc gactgatcca aaacatggat gccaccactg 301 agccttgtac agactttttc aaatatgctt gcggaggctg gttgaaacgt aatgtcattc 361 ccgagaccag ctcccgttac ggcaactttg acattttaag agatgaacta gaagtcgttt 421 tgaaagatgt ccttcaagaa cccaaaactg aagatatagt agcagtgcag aaagcaaaag 481 cattgtacag gtcttgtata aatgaatctg ctattgatag cagaggtgga gaacctctac 541 tcaaactgtt accagacata tatgggtggc cagtagcaac agaaaactgg gagcaaaaat 601 atggtgcttc ttggacagct gaaaaagcta ttgcacaact gaattctaaa tatgggaaaa 661 aagtccttat taatttgttt gttggcactg atgataagaa ttctgtgaat catgtaattc 721 atattgacca acctcgactt ggcctccctt ctagagatta ctatgaatgc actggaatct 781 ataaagaggc ttgtacagca tatgtggatt ttatgatttc tgtggccaga ttgattcgtc 841 aggaagaaag attgcccatc gatgaaaacc agcttgcttt ggaaatgaat aaagttatgg 901 aattggaaaa agaaattgcc aatgctacgg ctaaacctga agatcgaaat gatccaatgc 961 ttctgtataa caagatgaca ttggcccaga tccaaaataa cttttcacta gagatcaatg 1021 ggaagccatt cagctggttg aatttcacaa atgaaatcat gtcaactgtg aatattagta 1081 ttacaaatga ggaagatgtg gttgtttatg ctccagaata tttaaccaaa cttaagccca 1141 ttcttaccaa atattctgcc agagatcttc aaaatttaat gtcctggaga ttcataatgg 1201 atcttgtaag cagcctcagc cgaacctaca aggagtccag aaatgctttc cgcaaggccc 1261 tttatggtac aacctcagaa acagcaactt ggagacgttg tgcaaactat gtcaatggga 1321 atatggaaaa tgctgtgggg aggctttatg tggaagcagc atttgctgga gagagtaaac 1381 atgtggtcga ggatttgatt gcacagatcc gagaagtttt tattcagact ttagatgacc 1441 tcacttggat ggatgccgag acaaaaaaga gagctgaaga aaaggcctta gcaattaaag 1501 aaaggatcgg ctatcctgat gacattgttt caaatgataa caaactgaat aatgagtacc 1561 tcgagttgaa ctacaaagaa gatgaatact tcgagaacat aattcaaaat ttgaaattca 1621 gccaaagtaa acaactgaag aagctccgag aaaaggtgga caaagatgag tggataagtg 1681 gagcagctgt agtcaatgca ttttactctt caggaagaaa tcagatagtc ttcccagccg 1741 gcattctgca gccccccttc tttagtgccc agcagtccaa ctcattgaac tatgggggca 1801 tcggcatggt cataggacac gaaatcaccc atggcttcga tgacaatggc agaaacttta 1861 acaaagatgg agacctcgtt gactggtgga ctcaacagtc tgcaagtaac tttaaggagc 1921 aatcccagtg catggtgtat cagtatggaa acttttcctg ggacctggca ggtggacagc 1981 accttaatgg aattaataca ctgggagaaa acattgctga taatggaggt cttggtcaag 2041 catacagagc ctatCAGAAT TATATTAAAA AGAATGGCGA AGAAAAATTA CTTCCTGGAC 2101 TTGACCTAAA TCACAAACAA CTATTTTTCT TGAACTTTGC ACAGGTGTGG TGTGGAACCT 2161 ATAGGCCAGA GTATGCGGTT AACTCCATTA AAACAGATGT GCACAGTCCA GGCAATTTCA 2221 GGATTATTGG GACTTTGCAG AACTCTGCAG AGTTTTCAGA AGCCTTTCAC TGCCGCAAGA 2281 ATTCATACAT GAATCCAGAA AAGAAGTGCC GGGTTTGGTT GCCAACTTTC TTGTACAAAG 2341 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2401 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2461 ACGATGAGCA TTCACCCTCA CACGGAAAAC GCGTTAAGTC gacaatcaac ctctggatta 2521 caaaatttgt gaaagatt