Construct: ORF TRCN0000475908
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006879.1_s317c1
- Derived from:
- ccsbBroadEn_06433
- DNA Barcode:
- GCCCGTTTAGAATTCAGGCAAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGHMBP2 (3508)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475908
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3508 | IGHMBP2 | immunoglobulin mu DNA bindi... | NM_002180.2 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 3508 | IGHMBP2 | immunoglobulin mu DNA bindi... | XR_949903.3 | 71.9% | (many diffs) | |
| 3 | human | 3508 | IGHMBP2 | immunoglobulin mu DNA bindi... | XM_005273974.2 | 65.9% | 65.9% | 0_1ins1011;1000A>G;1305C>T |
| 4 | human | 3508 | IGHMBP2 | immunoglobulin mu DNA bindi... | XM_017017669.2 | 65.9% | 65.9% | 0_1ins1011;1000A>G;1305C>T |
| 5 | human | 3508 | IGHMBP2 | immunoglobulin mu DNA bindi... | XM_017017670.2 | 65.9% | 65.9% | 0_1ins1011;1000A>G;1305C>T |
| 6 | human | 3508 | IGHMBP2 | immunoglobulin mu DNA bindi... | XM_005273975.3 | 61% | 58% | (many diffs) |
| 7 | human | 3508 | IGHMBP2 | immunoglobulin mu DNA bindi... | XM_011544994.1 | 58.5% | 58.4% | (many diffs) |
| 8 | human | 3508 | IGHMBP2 | immunoglobulin mu DNA bindi... | XM_017017671.2 | 54.9% | 54.6% | (many diffs) |
| 9 | human | 3508 | IGHMBP2 | immunoglobulin mu DNA bindi... | XM_005273976.2 | 49.6% | 43.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3048
- ORF length:
- 2979
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctcggca gctgtggaga gcttcgtgac caagcaactg gacctgctgg 121 agctcgagag agacgcggag gtggaggagc gcaggtcctg gcaggagaac atctctctga 181 aagagctcca gagccgaggc gtgtgtttgc tgaagctgca ggtatccagc cagcgcactg 241 ggctgtacgg acggctgctg gtcacctttg agcccaggcg atacgggtcc gcggcagctc 301 ttcccagtaa cagctttact tctggtgata tcgtgggcct gtacgatgct gctaatgagg 361 gcagtcagct ggccactggg atcttgaccc gggtcaccca gaagtcggtc acggtggcct 421 ttgatgagtc ccacgatttc cagttgagct tggaccgaga gaattcctac agactgttaa 481 aacttgccaa tgatgtcact tacaggcgac tgaaaaaagc cctgattgct ctaaagaagt 541 atcattctgg cccagcctcc tcactcatag aagtgctctt tggcagatct gctcccagtc 601 ctgccagtga aatacacccg ctgacattct tcaacacctg cctggacacc tcccagaaag 661 aagcggtttc atttgcgctg tctcagaaag aacttgccat catccatgga cctcctggca 721 ctgggaaaac cacgactgtg gttgagatca ttcttcaagc tgtgaaacaa ggcttaaagg 781 ttctgtgctg cgccccctcc aacatcgccg tggacaatct ggtggagcgc ctggctctgt 841 gtaagcagcg gattctgcgc ctgggacacc ctgcccgcct cctggagtcc attcagcagc 901 actccctgga tgcggtttta gcgcggagcg acagtgccca gattgttgca gatatcagga 961 aggacatcga ccaggtcttt gtgaaaaaca aaaagaccca ggataagaga gagaaaagta 1021 attttcgaaa tgaaattaag ctgttaagaa aagaactgaa ggagagggaa gaagcagcta 1081 tgctcgagag cctcacttcg gcaaacgtgg tccttgcaac aaacacaggt gcgtctgccg 1141 atggccccct gaagttgctg cccgagagct acttcgacgt ggtggtcatt gacgagtgtg 1201 cccaggccct cgaggcgagc tgctggatcc ccctgctgaa ggccagaaag tgcatcctgg 1261 cgggcgatca caagcagctg ccccccacca cagtctctca caaggctgcg ctggcaggac 1321 tgtcactcag cctgatggaa cgcctggctg aggagtacgg cgcgagggtg gtgcggacac 1381 tgacggtgca gtaccgcatg caccaggcta tcatgcgctg ggcctcagac accatgtacc 1441 ttgggcagct cacagcccac tcttccgtgg caaggcacct cctgagggac ctcccaggtg 1501 tggctgccac agaagagacg ggtgtgcccc tgctcttggt ggacaccgcc ggctgcgggc 1561 tgtttgagct ggaggaggag gacgaacagt cgaaagggaa ccctggcgaa gtccgcctcg 1621 tcagtttgca catccaggct ctggtggacg ctggtgttcc agcccgtgac attgctgtgg 1681 tctcgccata caacctccag gtggacctgc tcagacagag ccttgtgcac aggcaccctg 1741 agcttgaaat caagtctgtc gatggcttcc aaggccgaga gaaggaggcc gtgatactgt 1801 ccttcgtcag atccaacagg aaaggtgaag ttggttttct tgctgaggac cggaggatca 1861 acgtggctgt cacccgtgcc cgacgccacg tggcggtcat ctgtgactcc cgtactgtca 1921 acaaccatgc atttttgaag accctggtgg agtatttcac acagcatggg gaagtacgca 1981 cggcctttga gtatcttgac gatattgtcc cagaaaacta ttcccatgag aactcccagg 2041 gttccagcca cgctgccacc aagccccagg gacctgctgc gtccaccagg accggaagcc 2101 agcggcagga gggaggccag gaggctgcag cacctgccag acagggccgg aagaagccgg 2161 ctgggaagtc tctggcctct gaagctccat ctcagcccag cctcaacgga ggcagcccag 2221 agggagtgga gagccaagat ggcgtggacc acttccgggc catgatagtg gagttcatgg 2281 ccagcaagaa gatgcagttg gagtttcctc cttccctcaa ttcccacgac aggctgcggg 2341 tccaccaaat agccgaggag cacgggctga ggcacgacag ttctggggaa gggaagagga 2401 ggttcatcac tgtgagcaag agggccccgc gaccccgagc agccctggga cccccagcag 2461 ggaccggtgg cccagcccct ctccagccag tgccccctac ccctgcgcag acagagcagc 2521 ctcccaggga gcagcgtggc ccagaccagc ctgatctgag gacgctgcac ctggagagac 2581 tgcagagggt caggagcgcg caggggcagc ccgccagcaa ggagcagcag gcctcagggc 2641 agcagaaact tccagaaaag aaaaagaaaa aagccaaagg acatccggcc acagatctgc 2701 ccacggagga ggactttgag gccctggttt ctgccgccgt taaggctgat aacaccTGCG 2761 GCTTTGCCAA GTGCACAGCC GGCGTCACAA CCCTGGGCCA GTTCTGCCAG CTCTGCAGCC 2821 GCCGCTACTG CCTCAGCCAC CACCTGCCCG AGATCCATGG CTGCGGTGAG AGGGCTCGCG 2881 CCCATGCCCG GCAGAGAATC AGCCGGGAAG GGGTCCTCTA TGCCGGCAGC GGGACCAAGA 2941 ACGGATCCCT GGACCCAGCC AAGAGGGCCC AGCTGCAGAG GAGGCTGGAT AAGAAGCTGA 3001 GTGAGCTCAG CAACCAGAGG ACCAGCCGGA GGAAGGAGAG GGGGACGTTG CCAACTTTCT 3061 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3121 CGTAGTAATG AACTAGTCCG TAACTTAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 3181 TGGAAAGGAC GAGCCCGTTT AGAATTCAGG CAAGCGACGC GTTAAGTCga caatcaacct 3241 ctggattaca aaatttgtga aagatt