Construct: ORF TRCN0000475909

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000025.1_s317c1
Derived from:
ccsbBroadEn_04171
DNA Barcode:
ACCAAGAGATGATGTTGTTTATAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEMA6D (80031)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475909
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80031 SEMA6D semaphorin 6D NM_001198999.2 100% 100%
2 human 80031 SEMA6D semaphorin 6D NM_020858.2 100% 100%
3 human 80031 SEMA6D semaphorin 6D XM_011522080.2 100% 100%
4 human 80031 SEMA6D semaphorin 6D XM_024450076.1 100% 100%
5 human 80031 SEMA6D semaphorin 6D NM_153616.2 98.7% 98.7% 1646_1647ins39
6 human 80031 SEMA6D semaphorin 6D XM_011522081.2 98.7% 98.7% 1646_1647ins39
7 human 80031 SEMA6D semaphorin 6D XM_017022621.1 98.7% 98.7% 1646_1647ins39
8 human 80031 SEMA6D semaphorin 6D XM_011522078.2 98.1% 98.1% 1746_1802del
9 human 80031 SEMA6D semaphorin 6D XM_017022619.2 98.1% 98.1% 1746_1802del
10 human 80031 SEMA6D semaphorin 6D NM_153617.2 96.8% 96.8% 1646_1647ins39;1707_1763del
11 human 80031 SEMA6D semaphorin 6D XM_011522079.2 96.8% 96.8% 1646_1647ins39;1707_1763del
12 human 80031 SEMA6D semaphorin 6D XM_017022620.1 96.8% 96.8% 1646_1647ins39;1707_1763del
13 human 80031 SEMA6D semaphorin 6D XM_011522076.2 94.7% 94.7% 1747_1914del
14 human 80031 SEMA6D semaphorin 6D XM_017022618.2 94.7% 94.7% 1747_1914del
15 human 80031 SEMA6D semaphorin 6D XM_005254689.4 93.5% 93.5% 1646_1647ins39;1708_1875del
16 human 80031 SEMA6D semaphorin 6D XM_011522077.2 93.5% 93.5% 1646_1647ins39;1708_1875del
17 human 80031 SEMA6D semaphorin 6D NM_001358352.2 93% 93% 1747_1971del
18 human 80031 SEMA6D semaphorin 6D XM_005254685.4 93% 93% 1747_1971del
19 human 80031 SEMA6D semaphorin 6D XM_011522075.2 93% 93% 1747_1971del
20 human 80031 SEMA6D semaphorin 6D XM_017022617.2 93% 93% 1747_1971del
21 human 80031 SEMA6D semaphorin 6D XM_024450073.1 93% 93% 1747_1971del
22 human 80031 SEMA6D semaphorin 6D XM_024450074.1 93% 93% 1747_1971del
23 human 80031 SEMA6D semaphorin 6D XM_024450075.1 93% 93% 1747_1971del
24 human 80031 SEMA6D semaphorin 6D NM_001358351.2 91.8% 91.8% 1646_1647ins39;1708_1932del
25 human 80031 SEMA6D semaphorin 6D NM_153618.2 91.8% 91.8% 1646_1647ins39;1708_1932del
26 human 80031 SEMA6D semaphorin 6D XR_001751399.1 59.8% (many diffs)
27 human 80031 SEMA6D semaphorin 6D NM_153619.1 57.2% 55.2% 1646_1647ins39;1706_1740del;1791_1792ins1238
28 human 80031 SEMA6D semaphorin 6D NM_024966.3 47% 47% 1428_1429ins1605
29 mouse 214968 Sema6d sema domain, transmembrane ... NM_172537.4 88.5% 95.2% (many diffs)
30 mouse 214968 Sema6d sema domain, transmembrane ... NM_001290997.1 87.3% 93.9% (many diffs)
31 mouse 214968 Sema6d sema domain, transmembrane ... NM_199238.3 87.3% 93.9% (many diffs)
32 mouse 214968 Sema6d sema domain, transmembrane ... NM_199239.3 86.9% 93.4% (many diffs)
33 mouse 214968 Sema6d sema domain, transmembrane ... XM_017317019.1 86.9% 93.4% (many diffs)
34 mouse 214968 Sema6d sema domain, transmembrane ... XM_017317021.1 85.7% 92.2% (many diffs)
35 mouse 214968 Sema6d sema domain, transmembrane ... XM_017317010.1 83.9% 90.2% (many diffs)
36 mouse 214968 Sema6d sema domain, transmembrane ... XM_017317014.1 83% 89.3% (many diffs)
37 mouse 214968 Sema6d sema domain, transmembrane ... XM_017316995.1 82.7% 89% (many diffs)
38 mouse 214968 Sema6d sema domain, transmembrane ... NM_199240.3 82.7% 89% (many diffs)
39 mouse 214968 Sema6d sema domain, transmembrane ... XM_011239427.2 82.7% 89% (many diffs)
40 mouse 214968 Sema6d sema domain, transmembrane ... XM_017317012.1 82.7% 89% (many diffs)
41 mouse 214968 Sema6d sema domain, transmembrane ... XM_006499111.3 82.4% 88.6% (many diffs)
42 mouse 214968 Sema6d sema domain, transmembrane ... XM_006499112.3 82.4% 88.6% (many diffs)
43 mouse 214968 Sema6d sema domain, transmembrane ... XM_006499113.3 82.4% 88.6% (many diffs)
44 mouse 214968 Sema6d sema domain, transmembrane ... XM_006499114.3 82.4% 88.6% (many diffs)
45 mouse 214968 Sema6d sema domain, transmembrane ... NM_001291000.1 81.6% 87.8% (many diffs)
46 mouse 214968 Sema6d sema domain, transmembrane ... NM_199241.3 81.3% 87.4% (many diffs)
47 mouse 214968 Sema6d sema domain, transmembrane ... XM_017317001.1 81.3% 87.4% (many diffs)
48 mouse 214968 Sema6d sema domain, transmembrane ... XM_017317026.1 55.2% 55.3% (many diffs)
49 mouse 214968 Sema6d sema domain, transmembrane ... XM_017317028.1 54.7% 55% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3102
ORF length:
3033
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gagggtcttc ctgctttgtg cctacatact gctgctgatg gtttcccagt
121tgagggcagt cagctttcct gaagatgatg aaccccttaa tactgtcgac tatcactatt
181caaggcaata tccggttttt agaggacgcc cttcaggcaa tgaatcgcag cacaggctgg
241actttcagct gatgttgaaa attcgagaca cactttatat tgctggcagg gatcaagttt
301atacagtaaa cttaaatgaa atgcccaaaa cagaagtaat acccaacaag aaactgacat
361ggcgatcaag acaacaggat cgagaaaact gtgctatgaa aggcaagcat aaagatgaat
421gccacaactt tatcaaagta tttgttccaa gaaacgatga gatggttttt gtttgtggta
481ccaatgcatt caatcccatg tgtagatact acaggttgag taccttagaa tatgatgggg
541aagaaattag tggcctggca agatgcccat ttgatgccag acaaaccaat gttgccctct
601ttgctgatgg gaagctgtat tctgccacag tggctgactt cttggccagc gatgccgtta
661tttatcgaag catgggtgat ggatctgccc ttcgcacaat aaaatatgat tccaaatgga
721taaaagagcc acactttctt catgccatag aatatggaaa ctatgtctat ttcttctttc
781gagaaatcgc tgtcgaacat aataatttag gcaaggctgt gtattcccgc gtggcccgca
841tatgtaaaaa cgacatgggt ggttcccagc gggtcctgga gaaacactgg acttcatttc
901taaaggctcg gctgaactgt tctgtccctg gagattcgtt tttctacttt gatgttctgc
961agtctattac agacataata caaatcaatg gcatccccac tgtggtcggg gtgtttacca
1021cgcagctcaa tagcatccct ggttctgctg tctgtgcatt tagcatggat gacattgaaa
1081aagtattcaa aggacggttt aaggaacaga aaactccaga ttctgtttgg acagcagttc
1141ccgaagacaa agtgccaaag ccaaggcctg gctgttgtgc aaaacacggc cttgccgaag
1201cttataaaac ctccatcgat ttcccggatg aaactctgtc attcatcaaa tctcatcccc
1261tgatggactc tgccgttcca cccattgccg atgagccctg gttcacaaag actcgggtca
1321ggtacagact gacggccatc tcagtggacc attcagccgg accctaccag aactacacag
1381tcatctttgt tggctctgaa gctggcatgg tacttaaagt tctggcaaag accagtcctt
1441tctctttgaa cgacagcgta ttactggaag agattgaagc ctacaaccat gcaaagtgca
1501gtgctgagaa tgaggaagac aaaaaggtca tctcattaca gttggataaa gatcaccacg
1561ctttatatgt ggcgttctct agctgcatta tccgcatccc cctcagtcgc tgtgagcgtt
1621atggatcatg taaaaagtct tgtattgcat ctcgtgaccc gtattgtggc tggttaagcc
1681agggatcctg tggtagagtg accccaggga tgctgctgtt aaccgaagac ttctttgctt
1741tccataacca cagtgctgaa ggatatgaac aagacacaga attcggcaac acagctcatc
1801taggggactg ccatggtgta cgatgggaag tccagtctgg agagtccaac cagatggtcc
1861acatgaatgt cctcatcacc tgtgtctttg ctgcttttgt tttgggggca ttcattgcag
1921gtgtggcagt atactgctat cgagacatgt ttgttcggaa aaacagaaag atccataaag
1981atgcagagtc cgcccagtca tgcacagact ccagtggaag ttttgccaaa ctgaatggtc
2041tctttgacag ccctgtcaag gaataccaac agaatattga ttctcctaaa ctgtatagta
2101acctgctaac cagtcggaaa gagctaccac ccaatggaga tactaaatcc atggtaatgg
2161accatcgagg gcaacctcca gagttggctg ctcttcctac tcctgagtct acacccgtgc
2221ttcaccagaa gaccctgcag gccatgaaga gccactcaga aaaggcccat ggccatggag
2281cttcaaggaa agaaacccct cagttttttc cgtctagtcc gccacctcat tccccattaa
2341gtcatgggca tatccccagt gccattgttc ttccaaatgc tacccatgac tacaacacgt
2401ctttctcaaa ctccaatgct cacaaagctg aaaagaagct tcaaaacatt gatcaccctc
2461tcacaaagtc atccagtaag agagatcacc ggcgttctgt tgattccaga aataccctca
2521atgatctcct gaagcatctg aatgacccaa atagtaaccc caaagccatc atgggagaca
2581tccagatggc acaccagaac ttaatgctgg atcccatggg atcgatgtct gaggtcccac
2641ctaaagtccc taaccgggag gcatcgctat actcccctcc ttcaactctc cccagaaata
2701gcccaaccaa gcgagtggat gtccccacca ctcctggagt cccaatgact tctctggaaa
2761gacaaagagg ttatcacaaa aattccTCCC AGAGGCACTC TATATCTGCT ATGCCTAAAA
2821ACTTAAACTC ACCAAATGGT GTTTTGTTAT CCAGACAGCC TAGTATGAAC CGTGGAGGAT
2881ATATGCCCAC CCCCACTGGG GCGAAGGTGG ACTATATTCA GGGAACACCA GTGAGTGTTC
2941ATCTGCAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACT CTTCCTAGGA
3001CGGGACTAAA GAGGACGCCG TCCTTAAAAC CTGACGTGCC ACCAAAGCCT TCCTTTGTTC
3061CTCAAACCCC ATCTGTCAGA CCACTGAACA AATACACATA CTTGCCAACT TTCTTGTACA
3121AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
3181AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
3241AGGACGAACC AAGAGATGAT GTTGTTTATA GACGCGTTAA GTCgacaatc aacctctgga
3301ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)