Construct: ORF TRCN0000475910

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006504.1_s317c1
Derived from:
ccsbBroadEn_05924
DNA Barcode:
GCTCCATTGATGAATATAAACAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CACNB2 (783)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475910
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 783 CACNB2 calcium voltage-gated chann... NM_201596.3 100% 100%
2 human 783 CACNB2 calcium voltage-gated chann... NM_201571.4 95% 94% (many diffs)
3 human 783 CACNB2 calcium voltage-gated chann... NM_201597.3 94.8% 93.6% (many diffs)
4 human 783 CACNB2 calcium voltage-gated chann... NM_201593.3 94% 93.7% (many diffs)
5 human 783 CACNB2 calcium voltage-gated chann... NM_201570.3 91.2% 90.4% (many diffs)
6 human 783 CACNB2 calcium voltage-gated chann... NM_000724.4 91% 90.1% (many diffs)
7 human 783 CACNB2 calcium voltage-gated chann... NM_201590.3 90.6% 88.7% (many diffs)
8 human 783 CACNB2 calcium voltage-gated chann... XM_006717502.3 90.2% 89.6% (many diffs)
9 human 783 CACNB2 calcium voltage-gated chann... NM_201572.4 89.9% 87.7% (many diffs)
10 human 783 CACNB2 calcium voltage-gated chann... NM_001167945.2 89.1% 87.8% (many diffs)
11 human 783 CACNB2 calcium voltage-gated chann... XM_011519659.2 85.5% 82.4% (many diffs)
12 human 783 CACNB2 calcium voltage-gated chann... XM_005252588.4 85.3% 84.2% (many diffs)
13 human 783 CACNB2 calcium voltage-gated chann... NM_001330060.2 85.1% 83.9% (many diffs)
14 human 783 CACNB2 calcium voltage-gated chann... XM_005252591.3 57.5% 57.5% 0_1ins840
15 human 783 CACNB2 calcium voltage-gated chann... XM_017016625.1 57.5% 57.5% 0_1ins840
16 human 783 CACNB2 calcium voltage-gated chann... XR_001747198.1 22.7% (many diffs)
17 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497313.3 89.8% 93.7% (many diffs)
18 mouse 12296 Cacnb2 calcium channel, voltage-de... NM_023116.4 89.6% 93.6% (many diffs)
19 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497315.3 86% 89.5% (many diffs)
20 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497314.3 85.8% 89.4% (many diffs)
21 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497318.3 84% 87.5% (many diffs)
22 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497317.3 83.8% 87.4% (many diffs)
23 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497319.3 82.6% 85.7% (many diffs)
24 mouse 12296 Cacnb2 calcium channel, voltage-de... NM_001309519.1 82.4% 85.6% (many diffs)
25 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497320.1 82.2% 85.3% (many diffs)
26 mouse 12296 Cacnb2 calcium channel, voltage-de... NM_001252533.1 82.1% 85% (many diffs)
27 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497321.3 80.2% 83.3% (many diffs)
28 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_011238946.2 76.4% 79.1% (many diffs)
29 mouse 12296 Cacnb2 calcium channel, voltage-de... XM_006497323.1 71.6% 74.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2049
ORF length:
1980
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggtccaaagg gacatgtcca agtcgcctcc cacagcggcg gcggcggtgg
121cgcaggagat ccagatggaa ctgctagaga acgtggctcc cgcgggggcg ctcggagccg
181ccgcacagtc atatggaaaa ggagccagaa ggaaaaacag atttaaagga tctgatggaa
241gcacgtcatc tgatactacc tcaaatagtt ttgttcgcca gggttcggca gactcctaca
301ctagccgtcc atccgattcc gatgtatctc tggaggagga ccgggaggca gtgcgcagag
361aagcggagcg gcaggcccag gcacagttgg aaaaagcaaa gacaaagccc gttgcatttg
421cggttcggac aaatgtcagc tacagtgcgg cccatgaaga tgatgttcca gtgcctggca
481tggccatctc attcgaagca aaagattttc tgcatgttaa ggaaaaattt aacaatgact
541ggtggatagg gcgattggta aaagaaggct gtgaaatcgg attcattcca agcccagtca
601aactagaaaa catgaggctg cagcatgaac agagagccaa gcaagggaaa ttctactcca
661gtaaatcagg aggaaattca tcatccagtt tgggtgacat agtacctagt tccagaaaat
721caacacctcc atcatctgct atagacatag atgctactgg cttagatgca gaagaaaatg
781atattccagc aaaccaccgc tcccctaaac ccagtgcaaa cagtgtaacg tcaccccact
841ccaaagagaa aagaatgccc ttctttaaga agacagagca cactcctccg tatgatgtgg
901taccttccat gcgaccagtg gtcctagtgg gcccttctct gaagggctac gaggtcacag
961atatgatgca aaaagcgctg tttgattttt taaaacacag atttgaaggg cggatatcca
1021tcacaagggt caccgctgac atctcgcttg ccaaacgctc ggtattaaac aatcccagta
1081agcacgcaat aatagaaaga tccaacacaa ggtcaagctt agcggaagtt cagagtgaaa
1141tcgaaaggat ttttgaactt gcaagaacat tgcagttggt ggtccttgac gcggatacaa
1201ttaatcatcc agctcaactc agtaaaacct ccttggcccc tattatagta tatgtaaaga
1261tttcttctcc taaggtttta caaaggttaa taaaatctcg agggaaatct caagctaaac
1321acctcaacgt ccagatggta gcagctgata aactggctca gtgtcctcca gagctgttcg
1381atgtgatctt ggatgagaac cagcttgagg atgcctgtga gcaccttgcc gactatctgg
1441aggcctactg gaaggccacc catcctccca gcagtagcct ccccaaccct ctccttagcc
1501gtacattagc cacttcaagt ctgcctctta gccccaccct agcctctaat tcacagggtt
1561ctcaaggtga tcagaggact gatcgctccg ctcctatccg ttctgcttcc caagctgaag
1621aagaacctag tgtggaacca gtcaagaaat cccagcaccg ctcttcctcc tcagccccac
1681accacaacca tcgcagtggg acaagtcgcg gcctctccag gcaagagaca tttgactcgg
1741aaacccagga gagtcgagac tctgccTACG TAGAGCCAAA GGAAGATTAT TCCCATGACC
1801ACGTGGACCA CTATGCCTCA CACCGTGACC ACAACCACAG AGACGAGACC CACGGGAGCA
1861GTGACCACAG ACACAGGGAG TCCCGGCACC GTTCCCGGGA CGTGGATCGA GAGCAGGACC
1921ACAACGAGTG CAACAAGCAG CGCAGCCGTC ATAAATCCAA GGATCGCTAC TGTGAAAAGG
1981ATGGAGAAGT GATATCAAAA AAACGGAATG AGGCTGGGGA GTGGAACAGG GATGTTTACA
2041TCCGCCAATT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
2101CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
2161TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCTCCA TTGATGAATA TAAACAACAC
2221GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)