Construct: ORF TRCN0000476080
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014001.1_s317c1
- Derived from:
- ccsbBroadEn_09688
- DNA Barcode:
- TACCGGACCTGCCAAGGTCGGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCFD2 (152579)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476080
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152579 | SCFD2 | sec1 family domain containi... | NM_152540.4 | 99.9% | 100% | 1458G>A |
| 2 | human | 152579 | SCFD2 | sec1 family domain containi... | XM_017007786.2 | 80.4% | 77.7% | (many diffs) |
| 3 | human | 152579 | SCFD2 | sec1 family domain containi... | XM_017007787.2 | 77% | 76.7% | (many diffs) |
| 4 | human | 152579 | SCFD2 | sec1 family domain containi... | XM_011534375.3 | 76.9% | 76% | (many diffs) |
| 5 | human | 152579 | SCFD2 | sec1 family domain containi... | XM_011534376.3 | 76.2% | 76% | 1458G>A;1561_1562ins22;1566_1567ins464 |
| 6 | human | 152579 | SCFD2 | sec1 family domain containi... | XM_011534378.3 | 64.8% | 64.1% | (many diffs) |
| 7 | human | 152579 | SCFD2 | sec1 family domain containi... | XR_001741148.2 | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2118
- ORF length:
- 2052
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgcctcgggc gtactgtcct ttacccagca aggatgggag caggtgctgg 121 ccaaagtgaa acgggctgtg gtttacctgg acgccgcctg cgccgagagc ctgcactggg 181 gctgcggatc cacccgtctc ctggaggcgg tggggggtcc tgactgtcac ctgcgagagt 241 tcgagcccga cgcaattggt ggtggagcca agcagcccaa ggcagtgttt gtgctgagct 301 gcctgctgaa aggccggacc gtggagatcc tacgggacat catctgccgc agtcacttcc 361 agtattgtgt ggtggtcaca accgtgagcc acgctgtcca cctcacagct aatcatgtcc 421 cagcggcggc agcggccgag atggaggggc agcagccggt gttcgagcag ctggaggaga 481 agctgtgtga atggatgggc aacatgaact acacggccga ggtgttccat gtcccgttat 541 tgcttgcccc tgttgctccc cactttgcct tgactccagc ttttgcatcc cttttcccac 601 tgctacccca ggatgtgcac ctccttaata gcgcccgacc ggacaagagg aagctgggaa 661 gcctgggtga tgtggactcc actacgctaa ccccagagct gctgctgcag atcagatgcc 721 tagtgtcagg cctcagttct ctgtgtgaac atttaggagt acgggaggag tgttttgctg 781 taggttcctt aagtcaggtc atcgctgcgg atctggccaa ttatgcccct gcaaagaaca 841 ggaagaagac tgctgcaggc agggcatcag tggtttttgt ggacagaacc ctggatctca 901 caggagcagt tggacatcat ggagacaact tagtagagaa gatcatttca gcacttcccc 961 agctcccagg ccacacaaat gatgtgatgg ttaacatgat agcgctcact gcactccata 1021 ctgaggagga aaattataat gtggttgcac caggctgtct ttcacaatcc agtgacacca 1081 cagccaaagc cctatgggaa gctttactga acactaagca caaagaggca gtgatggaag 1141 ttcggagaca tctagtggaa gcggcaagca gagaaaacct gccaatcaag atgagtatgg 1201 ggagagtcac accgggacag ctcatgtcct atattcagct cttcaagaac aacctcaaag 1261 ctctaatgaa tcattgtggc ctcctccagc ttggactggc cacagctcaa acgttgaaac 1321 acccacagac tgccaagtgg gacaactttc tggcttttga aaggctcctt cttcagagca 1381 ttggggagtc agcaatgtcc gttgtgttaa atcagctgct gcccatgatt aagcctgtaa 1441 cccagagaac caacgaggac tacagccctg aggaactgct gatccttctc atatatattt 1501 attctgtcac tggagagctc acagtagaca aagacctgtg tgaagcagaa gaaaaagtca 1561 agaaagcatt ggctcaggtc ttctgtgagg aatctggatt gtcacctttg ctgcaaaaaa 1621 ttacggactg ggactcttca attaatctga catttcacaa atccaaaatt gccgtggatg 1681 aactctttac ttcacttcgg gatattgctg gagctcggag tctcctgaaa cagtttaagt 1741 ctgtatatgt tcctggaaat catacccacc aggcaTCTTA TAAGCCATTG TTGAAGCAAG 1801 TTGTGGAGGA AATATTTCAT CCCGAGAGGC CAGATTCCGT TGATATTGAA CACATGTCTT 1861 CAGGCCTCAC TGATCTCCTT AAAACTGGAT TTAGCATGTT CATGAAGGTG AGCCGGCCTC 1921 ATCCTAGTGA CTACCCCCTC CTGATCCTCT TTGTGGTAGG TGGGGTCACA GTCTCTGAAG 1981 TGAAAATGGT CAAAGATCTT GTGGCATCGT TGAAGCCAGG AACCCAGGTA ATCGTGCTGT 2041 CCACACGACT CCTGAAGCCA CTTAACATTC CTGAGCTGTT ATTTGCAACT GACCGACTGC 2101 ATCCAGACCT TGGCTTCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2161 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2221 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATACCGGA CCTGCCAAGG 2281 TCGGAATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt