Construct: ORF TRCN0000476227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017193.1_s317c1
- Derived from:
- ccsbBroadEn_13059
- DNA Barcode:
- CCTAAGGTCATGCATCTGCGGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C8orf34 (116328)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476227
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116328 | C8orf34 | chromosome 8 open reading f... | NM_052958.4 | 80.6% | 77% | (many diffs) |
| 2 | human | 116328 | C8orf34 | chromosome 8 open reading f... | NM_001349476.1 | 80.1% | 76.4% | (many diffs) |
| 3 | human | 116328 | C8orf34 | chromosome 8 open reading f... | NM_001349477.1 | 77.6% | 73.9% | (many diffs) |
| 4 | human | 116328 | C8orf34 | chromosome 8 open reading f... | NM_001349478.1 | 73% | 69.1% | (many diffs) |
| 5 | human | 116328 | C8orf34 | chromosome 8 open reading f... | NM_001349479.1 | 69.9% | 66.1% | (many diffs) |
| 6 | human | 116328 | C8orf34 | chromosome 8 open reading f... | XM_011517449.2 | 58.6% | 58.6% | 1_258del;1242_1243ins435 |
| 7 | human | 116328 | C8orf34 | chromosome 8 open reading f... | NM_001195639.2 | 51.2% | 50.4% | (many diffs) |
| 8 | human | 116328 | C8orf34 | chromosome 8 open reading f... | XM_011517450.2 | 50.5% | 50.4% | (many diffs) |
| 9 | human | 116328 | C8orf34 | chromosome 8 open reading f... | NM_001349480.1 | 43.5% | 42.5% | (many diffs) |
| 10 | human | 116328 | C8orf34 | chromosome 8 open reading f... | NR_146186.1 | 42% | (many diffs) | |
| 11 | human | 116328 | C8orf34 | chromosome 8 open reading f... | NR_146187.1 | 41% | (many diffs) | |
| 12 | human | 116328 | C8orf34 | chromosome 8 open reading f... | XR_928756.3 | 16.3% | (many diffs) | |
| 13 | mouse | 320492 | A830018L16Rik | RIKEN cDNA A830018L16 gene | NM_001160369.2 | 85% | 81.9% | (many diffs) |
| 14 | mouse | 320492 | A830018L16Rik | RIKEN cDNA A830018L16 gene | NM_177173.6 | 84.4% | 81.3% | (many diffs) |
| 15 | mouse | 320492 | A830018L16Rik | RIKEN cDNA A830018L16 gene | XM_017321221.1 | 82.1% | 79% | (many diffs) |
| 16 | mouse | 320492 | A830018L16Rik | RIKEN cDNA A830018L16 gene | NM_001310451.1 | 81.5% | 78.4% | (many diffs) |
| 17 | mouse | 320492 | A830018L16Rik | RIKEN cDNA A830018L16 gene | XM_006495540.2 | 76.4% | 73.2% | (many diffs) |
| 18 | mouse | 320492 | A830018L16Rik | RIKEN cDNA A830018L16 gene | XM_006495541.3 | 68% | 64.8% | (many diffs) |
| 19 | mouse | 320492 | A830018L16Rik | RIKEN cDNA A830018L16 gene | NM_001160371.2 | 54.1% | 56.2% | (many diffs) |
| 20 | mouse | 320492 | A830018L16Rik | RIKEN cDNA A830018L16 gene | NM_001160370.2 | 54.1% | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1488
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtctcat ccgcaaaccc ggatccaggc ttacctggag aagaacaaga 121 tcggtcccct gtttgaggaa ttaatgacca agttaataac tgagacacct gaccagccaa 181 tcccatttct cattgaccat cttcagtcta aacaagggaa ccgtggacaa cttcaaagaa 241 ctttgtctgg atctgcagct ctatgggcag aaagtgaaaa atcagaatcc aaaggaacaa 301 gaagggattt cagaagctat gataaacctt ggcaattaaa tgcaaagaag cctaaaaaat 361 caaaaagtga ccttgctgtg tctaatattt ctccaccatc accggactcc aaatcattgc 421 caaggtcagt agagcatcca aagtggaact ggaggactaa accacaaagc cgtgattttg 481 atgaattgaa tcacatcctt caggagagca agaagctggg gaaagccctt gagaatctct 541 ctcgaagtat tgcaatttca gatgaactcg ataaggaaac agtgacattt aattcttctc 601 ttctgaggcc ccgtgtgatt ggagaatgga ttggtagaga agaaaatgat gctgatcccc 661 tagctgctga aatgctacag cctccaattc caagaagcaa aaatgaccaa tgggaaagtg 721 aagatagtgg ctctagtcct gcaggaagct taaagatgga gcctaagaac aaaggattaa 781 aacagcagca acagcaacat aagaaactcc tggccgcaat gctctctcaa gattcttttg 841 agtccatcca cagccctacc ccatctgtaa cagaagaaga tattgataat gaagatgatg 901 caatggaatt gctggaggat cttaatgatt taagaatgga gggagtaaca accctggtac 961 cttctgggag caaatttaac caaggccgtc ctacttaccc tgctgagcct caggccaagg 1021 tcacactgaa catctgttca aggtgtgcca ggcttcaagg agacaaccTG GAAGAAAGGA 1081 CAGAAGAGTC ACTACCAATA CTCCATTCTC CAGATGAAAA AATCCCAGAT TCATTCGATT 1141 CCTTGCCTGG GACTGAAGAA GCACTAATGG AGGAGGGTGA CGAATTTGAG AAAGCATCTA 1201 AACTAACAGG ACCTGGAGAA GCCTCCAGTG GAGTAGGACA CTCACTGAAA AACTACATGG 1261 AAGAAGATGA ATCCTTAAAG CAATTGCAGG TAGTTCATCA ACCATGGATC TTGCCAAGTG 1321 ACACAGAAAG TGAAGGAGTG GAAACAGAAC AAGAAAACGT TCCGCTGATC TTCTTCTTTG 1381 CGTTCCATGC TCTTCTTGTC CTACGCTGGT CTACTCTGGT TTGTGAAACA GAGAGAAGAA 1441 GTTGCAAGTG GTCCTTAAAG AAATGCAGTT ATTCAAATCC TTATGTATTG CCAACTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGACCTAAGG TCATGCATCT GCGGTTAACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt