Construct: ORF TRCN0000476321
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015964.1_s317c1
- Derived from:
- ccsbBroadEn_05192
- DNA Barcode:
- CTGGATCGCTCCCCTCCTTGCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPRED2 (200734)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476321
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200734 | SPRED2 | sprouty related EVH1 domain... | NM_181784.3 | 99.9% | 99.7% | 877T>C |
| 2 | human | 200734 | SPRED2 | sprouty related EVH1 domain... | NM_001128210.2 | 98.4% | 97.8% | (many diffs) |
| 3 | human | 200734 | SPRED2 | sprouty related EVH1 domain... | XM_017003576.1 | 70.4% | 70% | 0_1ins366;3_3delGinsAGAA;508T>C |
| 4 | human | 200734 | SPRED2 | sprouty related EVH1 domain... | XM_005264200.5 | 54.2% | 45.9% | (many diffs) |
| 5 | human | 200734 | SPRED2 | sprouty related EVH1 domain... | XM_005264201.1 | 52.7% | 52.6% | 0_1ins591;286T>C |
| 6 | human | 200734 | SPRED2 | sprouty related EVH1 domain... | XM_005264202.5 | 49.4% | 47.3% | (many diffs) |
| 7 | mouse | 114716 | Spred2 | sprouty-related, EVH1 domai... | NM_033523.4 | 86.6% | 92.3% | (many diffs) |
| 8 | mouse | 114716 | Spred2 | sprouty-related, EVH1 domai... | XM_006514458.3 | 85.2% | 90.4% | (many diffs) |
| 9 | mouse | 114716 | Spred2 | sprouty-related, EVH1 domai... | XM_006514457.3 | 85% | 90.2% | (many diffs) |
| 10 | mouse | 114716 | Spred2 | sprouty-related, EVH1 domai... | XM_006514456.3 | 82.6% | 87.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1323
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgaagaa acacacccag acgatgacag ctatattgtg cgtgtcaagg 121 ctgtggttat gaccagagat gactccagcg ggggatggtt cccacaggaa ggaggcggga 181 tcagtcgcgt cggggtctgt aaggtcatgc accccgaagg caatggacga agcggctttc 241 tcatccatgg tgaacgacag aaagacaaac tggtggtatt ggaatgctat gtaagaaagg 301 acttggtcta caccaaagcc aatccaacgt ttcatcactg gaaggtcgat aataggaagt 361 ttggacttac tttccaaagc cctgctgatg cccgagcctt tgacagggga gtaaggaaag 421 caatcgaaga ccttatagaa ggttcaacaa cgtcatcttc caccatccat aatgaagctg 481 agcttggcga tgatgacgtt tttacaacag ctacagacag ttcttctaat tcctctcaga 541 agagagagca acctactcgg acaatctcct ctcccacatc ctgtgagcac cggaggattt 601 ataccctggg ccacctccac gactcatacc ccacagacca ctatcacctc gatcagccga 661 tgccaaggcc ctaccgccag gtgagcttcc cggacgacga cgaggagatc gtgcgcatca 721 acccccggga gaagatctgg atgacggggt acgaggatta ccggcacgca cccgtcaggg 781 gcaagtaccc ggacccctcg gaggacgcgg actcctccta cgtgcgcttc gccaagggcg 841 aggtccccaa gcatgactac aactacccct acgtggactc ctcagacttt ggcctaggcg 901 aggaccccaa aggccgcggg ggcagcgtga tcaagacgca gcccCCCCGG GGCAAGTCGC 961 GGCGGCGGAA GGAGGACGGA GAGCGCTCGC GGTGCGTGTA CTGCAGGGAC ATGTTCAACC 1021 ACGAGGAGAA CCGCCGGGGC CACTGCCAGG ACGCGCCCGA CTCCGTGAGA ACTTGCATCC 1081 GCCGGGTGAG CTGCATGTGG TGCGCGGACA GCATGCTCTA TCACTGTATG TCGGACCCCG 1141 AGGGAGACTA TACAGACCCT TGCTCGTGCG ATACTAGCGA CGAGAAGTTT TGCCTCCGGT 1201 GGATGGCTCT TATTGCCTTG TCTTTCCTGG CCCCCTGTAT GTGCTGTTAC CTGCCCCTTC 1261 GGGCCTGCTA CCACTGCGGA GTGATGTGCA GGTGCTGTGG CGGGAAGCAC AAAGCGGCCG 1321 CGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGACT GGATCGCTCC CCTCCTTGCG CCACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt